Immunohistochemistry Protocol (NBP1-18319)

IHC conditions for NBP1-18319:

Multiple 5 um serial sections (cervical intraepithelial neoplasia of mouse) from selected formalin-fixed, paraffin-embedded blocks were cut onto aminopropyltriethoxysilane-coated slides. The tissues were dewaxed in xylene and rehydrated. Heat-induced antigen retrieval in citrate buffer was followed by blockade of endogenous peroxidase activity with hydrogen peroxide. The sections were incubated with 10% goat serum in phosphate-buffered saline to reduce non-specific binding and background staining.