Immunohistochemistry Protocol (NB200-174)


1. Sections from a wild type mouse testis taken at P11 were probed with anti-p19Arf (NB 200-174) [green], after antigen retrieval.
2. Sections were stained with DAPI to reveal the position of cell nuclei [blue].
3. p19Arf staining can be seen in subnuclear structures (presumably nucleoli) in cells at the periphery of the testicular tubules.