Immunohistochemistry-Frozen protocol specific for Collagen III Antibody (NB100-92162)

For tissue stored at -80C:

Remove from freezer and equilibrate at -20C for approximately 15 minutes before attempting to section. This may prevent cracking of the block when sectioning.

- Section tissue at a range of 6-8 um and place on positively charged slides.
- Allow sections to air dry on bench for a few minutes before fixing (this helps sections adhere to slides).
- Fixing Tissue & Making Blocks:
- After tissue has been removed, fix in 4% paraformaldehyde for two hours at 4C