Immunocytochemistry/Immunofluorescence Protocol for p19ARF Antibody (NB200-174)

p19ARF/CDKN2A Antibody (5-C3-1):

1. Wild type mouse embryo fibroblasts (MEF) at passage 5 and NIH3T3 cells, which have deleted the Arf gene were fixed in methanol/acetone, and probed with anti-p19Arf (NB 200-174).
2. Cells were then probed with a fluorescently labeled anti-rat IgG secondary antibody.
3. Cells were also stained with Hoechst dye to reveal the position of nuclei.