Immunocytochemistry/Immunofluorescence Protocol for Influenza A H1N1 Antibody (NB200-414)

1) Cell monolayer was grown on a glass and infected with the virus for 2 days at 37C.
2) Glass was dried and the cells were fixed with cold acetone at 4C for 10 min.
3) NB 200-414 was introduced in concentrations from 1 to 100 ug/ml in PBS and incubated for 30 min in RT.
4) Glass was washed with PBS several times and incubated with goat anti-mouse IgG labeled with FITC.