Assay Procedure

Complete the surface staining for the cell surface molecules of interest. After the final wash, add the desired amount
of staining buffer to read on flow machine (generally between 0.25mL-0.5mL). To all tubes, except unstained cells,
add 2-5 ul 7-AAD per million cells and incubate for 10-15 minutes before analysis. It is recommended that along with
unstained cells, you also prepare cells with only 7-AAD for compensation purposes. 7-AAD is light sensitive; therefore
should be protected from light when being used.