Immunohistochemistry (IHC) Handbook

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Immunohistochemistry (IHC)

IHC Primary Antibody Selection & Optimization

Secondary Antibody Handbook

IHC Detection

IHC Microarrays

This Immunohistochemistry (IHC) Handbook provides a clear and concise introduction to IHC for researchers, new to the technique, or lab scientists in search of a quick refresher. Find a brief overview of IHC including technical tips, benchtop protocols, and a troubleshooting guide to optimize your IHC experiments.

ihc handbook

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Find answers to frequently asked questions:

  • What variables should I consider when designing an IHC experiment?
  • What are the advantages of using formalin-fixed, paraffin-embedded tissues over frozen sections and vice versa?
  • When is an antigen retrieval step necessary?
  • How can I reduce high background staining?
  • What are common signal amplification methods?

IHC Protocols:

  • Tissue Sample Preparation and Fixation
  • Antigen Retrieval
  • Chromogenic and Fluorescence Detection

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