Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.
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![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0001.jpg "PiColorlock Gold Colorimetric Assay Kit [303-0030] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.")
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate forms and settles. In plate assays, the data become extremely erratic when clumps of material form in the light path. PiColorlock complexes are stable for many hours, which allows multiple assay plates or cuvettes to be conveniently set up as a batch and read later using plate stackers or other automated equipment.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0002.jpg)
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor products, at least at low levels of inorganic phosphate. Competitors' products are linear over a much narrower range of concentrations. PiColorlock ALS reagent has now been superseded by PiColorlock Gold, which gives higher OD values.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0008.jpg)
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.](http://images.novusbio.com/fullsize2/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0007.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0003.jpg "PiColorLock Gold Colorimetric Assay Kit [303-0125] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.")
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate forms and settles. In plate assays, the data become extremely erratic when clumps of material form in the light path. PiColorlock complexes are stable for many hours, which allows multiple assay plates or cuvettes to be conveniently set up as a batch and read later using plate stackers or other automated equipment.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0004.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0005.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor products, at least at low levels of inorganic phosphate. Competitors' products are linear over a much narrower range of concentrations. PiColorlock ALS reagent has now been superseded by PiColorlock Gold, which gives higher OD values.](http://images.novusbio.com/fullsize2/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0006.jpg)
![pCLNDX Retrovirus Expression Vector [NBP2-29540]](http://images.novusbio.com/fullsize2/pCLNDX-Retrovirus-Expression-Vector-NBP2-29540-img0001.jpg "pCLNDX Retrovirus Expression Vector [NBP2-29540]")
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the mesoderm marker, Smooth Muscle Actin, was detected in 15 day cells using Mouse Anti-Smooth Muscle Actin monoclonal antibody (R&D Systems, Catalog # MAB1420). The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0013.jpg "Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the mesoderm marker, Smooth Muscle Actin, was detected in 15 day cells using Mouse Anti-Smooth Muscle Actin monoclonal antibody (R&D Systems, Catalog # MAB1420). The nuclei were counterstained with DAPI.")
![Flow Cytometry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Express the Pluripotency Stem Cell Marker SSEA-1 and Lack SSEA-4. v6.5 mouse embryonic stem cells (Novus Biologicals, NBP1-41162) were cultured in complete StemXVivo Mouse Pluripotent Stem Cell media. Expression of SSEA-1 and SSEA-4 were detected using PE-conjugated Mouse Anti-SSEA-1 Monoclonal Antibody (R&D Systems, Catalog # FAB2155P) and APC-conjugated Mouse Anti-SSEA-4 Monoclonal Antibody (R&D Systems, Catalog # FAB1435A. The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Flow-Cytometry-NBP1-41162-img0014.jpg)
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Express the Pluripotency Stem Cell Marker Oct-3/4. v6.5 mouse embryonic stem cells (Novus Biologicals, NBP1-41162) were cultured in complete StemXVivo Mouse Pluripotent Stem Cell media. Expression of Oct-3/4 was detected using Rat Anti-Human/Mouse Oct-3/4 Monoclonal Antibody (R&D Systems, Catalog # MAB1759) followed by NorthernLights (NL)557-conjugated Goat Anti-Rat Secondary Antibody (R&D Systems, Catalog # NL013). The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0009.jpg)
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Express the Pluripotency Stem Cell Marker Alkaline Phosphatase. v6.5 mouse embryonic stem cells (Novus Biologicals, NBP1-41162) were cultured in complete StemXVivo Mouse Pluripotent Stem Cell media. Expression of Alkaline Phosphatase was detected using Goat Anti-Mouse Alkaline Phosphatase Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF2910) followed by NL557-conjugated Donkey Anti-Goat Secondary Antibody (R&D Systems, Catalog # NL001). The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0010.jpg)
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the ectoderm marker, SOX1, was detected in day 10 cells using Goat Anti-SOX1 Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF3369) followed by NorthernLights (NL)557-conjugated Donkey Anti-Goat Secondary Antibody. The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0011.jpg)
![In vitro assay: v6.5 Mouse embryonic stem cells [NBP1-41162] - Brightfield Image of v6.5 Mouse embryonic stem cell [NBP1-41162] colonies growing on a Mitomycin C treated MEF cell feeder layer.](http://images.novusbio.com/fullsize2/v6.5 Mouse embryonic stem cells-In vitro assay-NBP1-41162-img0003.jpg)
![In vitro assay: v6.5 Mouse embryonic stem cells [NBP1-41162] - Brightfield Image of v6.5 Mouse embryonic stem cell [NBP1-41162] colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize2/v6.5 Mouse embryonic stem cells-In vitro assay-NBP1-41162-img0004.jpg)
![Immunodiffusion: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the endoderm marker, SOX17, was detected in 10 day cells using Goat Anti-SOX17 Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF1924) followed by NL557-conjugated Donkey Anti-Goat Secondary Antibody. The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunodiffusion-NBP1-41162-img0012.jpg)
![CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and TLR9/HEK 293 cell lines were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.](http://images.novusbio.com/fullsize2/CpG-oligodeoxynucleotides-with-negative-control--TLR9-ligand-NBP2-26232-img0002.jpg "CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and TLR9/HEK 293 cell lines were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.")
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.](http://images.novusbio.com/fullsize2/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0004.jpg "Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.")
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Evaluation of the ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line. IML-102 cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase reporter gene under the transcriptional control of an NF-KB response element. IML-102 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: specifically activated the TLR2/1-depedent NF-KB/SEAP reporter cells in a dose dependent manner (Note: HEK 293 cells endogenously express TLR1).](http://images.novusbio.com/fullsize2/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0005.jpg)
![PiBind Resin [501-0015]](http://images.novusbio.com/fullsize/p_59886_1.jpg "PiBind Resin [501-0015]")
![Red Blood Cell (RBC) Lysis Buffer [NBP2-29442] - data shows before (left) and after (right) human whole blood cells were lysed using the Red Blood Cell (RBC) lysis buffer. Image from verified customer review.](http://images.novusbio.com/fullsize2/Red-Blood-Cell-(RBC)-Lysis-Buffer-NBP2-29442-img0002.jpg "Red Blood Cell (RBC) Lysis Buffer [NBP2-29442] - data shows before (left) and after (right) human whole blood cells were lysed using the Red Blood Cell (RBC) lysis buffer. Image from verified customer review.")
![Polyinosinic-polycytidylic acid [NBP2-25288] - 293T cells were co-transfected with pCMV/TLR3 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000 (Life Technology). After 48 hrs of transfection, 10 ug/ml of Poly(I:C) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit](http://images.novusbio.com/fullsize2/Polyinosinic-polycytidylic-acid-NBP2-25288-img0002.jpg "Polyinosinic-polycytidylic acid [NBP2-25288] - 293T cells were co-transfected with pCMV/TLR3 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000 (Life Technology). After 48 hrs of transfection, 10 ug/ml of Poly(I:C) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit")