| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52716-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75902SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52661-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12075-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09029-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75901SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52648-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52682-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81100-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09015-0.025mg | |||
![Immunohistochemistry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-75902] - Formalin fixed rat spleen slices were were stained with a NBP2-75902 at 5 ug/ml. The anti-human CD25 antibody NBP2-75902 also shows some cross-reactivity with rat in IHC.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Immunohistochemistry-NBP2-75902-img0001.jpg "Immunohistochemistry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-75902] - Formalin fixed rat spleen slices were were stained with a NBP2-75902 at 5 ug/ml. The anti-human CD25 antibody NBP2-75902 also shows some cross-reactivity with rat in IHC.")
![Immunocytochemistry/Immunofluorescence: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-75902] - Formaldehyde-fixed rat thymus slices were stained with NBP2-75902 at 5 ug/ml and detected with a FITC-conjugated secondary antibody. Ab00188 shows weak labelling of rat cells by IF.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Immunofluorescence-NBP2-75902-img0002.jpg)
![Western Blot: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric - Azide and BSA Free [NBP2-75902] - Human thyroid cancer (lane 1), rat spleen (lane 2), mouse lymph node (lane 3), mouse thymus (lane 4) and rat small intestines (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75902 at 1 ug/ml before being detected by a secondary antibody.](http://images.novusbio.com/fullsize/nbp2-75902_human-monoclonal-cd25-il-2r-alpha-antibody-basiliximab-chimeric-74202522141245.png)
![Flow Cytometry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-52661] - Flow-cytometry using anti-CD25 antibody Basiliximab (NBP2-52661) Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Flow-Cytometry-NBP2-52661-img0007.jpg "Flow Cytometry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-52661] - Flow-cytometry using anti-CD25 antibody Basiliximab (NBP2-52661) Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-52661] - Flow-cytometry using the anti-CD25 (IL2R) research biosimilar antibody Basiliximab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Flow-Cytometry-NBP2-52661-img0005.jpg)
![Flow Cytometry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-52661] - Flow-cytometry using anti-CD25 antibody Basiliximab (NBP2-52661) Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Basiliximab-Chimeric-Flow-Cytometry-NBP2-52661-img0006.jpg)
![Immunocytochemistry /Immunofluorescence: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with NBP3-43539 at 10 ug/ml for 1h followed by Alexa Fluor488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43539, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-7420252226526.png "Immunocytochemistry /Immunofluorescence: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with NBP3-43539 at 10 ug/ml for 1h followed by Alexa Fluor488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43539, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor488 secondary antibody.")
![Flow Cytometry: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Staining of rat (Rattus norvegicus) leukocytes with either an isotype control (A) or NBP3-43539(B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an Alexa Fluor 488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-74202522141218.png)
![Western Blot: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Rat spleen (A), rat thymus (B) and rat lymph node (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with NBP3-43539 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD74 is ~24.5-31.6kDa, depending on the isoform and post-translational modification. NBP3-43539 successfully detected CD74 in rat spleen, thymus and lymph node lysates.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-74202522113030.png)
![Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-84-3C1-Chimeric-Western-Blot-NBP3-12075-img0001.jpg "Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 84-3C1 (NBP3-12075) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12075, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-84-3C1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12075-img0002.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free [NBP3-12075] - K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12075 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12075_rabbit-monoclonal-cd43-sialophorin-antibody-84-3c1-chimeric-74202522141237.png)
![Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-HMHV-1B18-Chimeric-Western-Blot-NBP3-09029-img0001.jpg "Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of HMHV-1B18 (NBP3-09029) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09029, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-HMHV-1B18-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09029-img0002.jpg)
![Flow Cytometry: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free [NBP3-09029] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-09029 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-09029_rabbit-monoclonal-hvem-tnfrsf14-antibody-hmhv-1b18-chimeric-74202522141222.png)
![Western Blot: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-75901] - Mouse lipocyte (lane 1), mouse spleen (lane 2), mouse thymus (lane 3), mouse lymph node (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75901 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (31 kDa) is seen in the human-derived sample, as well as further bands that are specific to human.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Daclizumab-Western-Blot-NBP2-75901-img0002.jpg "Western Blot: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-75901] - Mouse lipocyte (lane 1), mouse spleen (lane 2), mouse thymus (lane 3), mouse lymph node (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75901 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (31 kDa) is seen in the human-derived sample, as well as further bands that are specific to human.")
![Immunohistochemistry: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-75901] - Formalin fixed rat spleen slices were were stained with NBP2-75901 at 5 ug/ml.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Daclizumab-Immunohistochemistry-NBP2-75901-img0001.jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0003.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0001.jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0002.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-3-Chimeric-Immunohistochemistry-NBP2-52682-img0001.jpg "Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.")
![Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Flow-cytometry using the anti-CD30 (Tumor necrosis factor receptor superfamily member 8) antibody Ki-3. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Ki-3 (NBP2-52682, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-3-Chimeric-Flow-Cytometry-NBP2-52682-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (IDEC-114 (Galiximab)) - Chimeric [NBP2-81100] - Immunofluorescence staining of fixed Daudi cells with B7-1/CD80 Antibody (IDEC-114 (Galiximab)) [NBP2-81100]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) [NBP2-81100] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81100, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-IDEC-114-Galiximab-Chimeric-Immunocytochemistry-Immunofluorescence-NBP2-81100-img0001.jpg "Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (IDEC-114 (Galiximab)) - Chimeric [NBP2-81100] - Immunofluorescence staining of fixed Daudi cells with B7-1/CD80 Antibody (IDEC-114 (Galiximab)) [NBP2-81100]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) [NBP2-81100] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81100, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of P7/7 (NBP3-09015) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09015, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-P7-7-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09015-img0002.jpg "Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of P7/7 (NBP3-09015) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09015, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of P7/7 (NBP3-09015, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-P7-7-Chimeric-Flow-Cytometry-NBP3-09015-img0001.jpg)