| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52709-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62566-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52666-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52716-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52680-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09025-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52636-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52645-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52637-0.025mg | |||
![Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [PE] [NBP2-52709PE] - Mk2 cell line was infected with ZIKA virus at 72 p.o.i and the sample was stained and analyzed by flow cytometry. Image from verified customer review. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/Flavivirus-group-antigen-Antibody-D1-4G2-4-15-4G2-Flow-Cytometry-NBP2-52709-img0003.jpg)
![Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Western-Blot-NBP2-62566-img0002.jpg "Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.")
![Immunohistochemistry-Paraffin: APP Antibody (6E10) - Chimeric [NBP2-62566] - Staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody. 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 (NBP2-62566). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62566 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Immunohistochemistry-Paraffin-NBP2-62566-img0001.jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - 6E10 staining reveals amyloid plaque in frontal cortex of AD mouse model. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-284202314243..jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - Plaque staining in AD Mouse model (Hippocampus) - without formic acid pretreatment. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-672023103423..jpg)
![Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Left: Uninfected cells. Right: Cells infected with yellow fever 17D virus, MOI=1. Image from verified customer review. Analysis using NBP2-52666F conjugate.](http://images.novusbio.com/fullsize/Flavivirus-group-antigen-Antibody-D1-4G2-4-15-4G2-Chimeric-Flow-Cytometry-NBP2-52666-img0005.jpg "Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Left: Uninfected cells. Right: Cells infected with yellow fever 17D virus, MOI=1. Image from verified customer review. Analysis using NBP2-52666F conjugate.")
![Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Left panel: uninfected VeroB4 cells stained with 4G2 antibody at 1:400. Right panel: VeroB4 cells infected with Yellow-Fever 17D-Venus virus, stained with 4G2 antibody at 1:400. The signal from the 4G2 antibody correlates well with the Venus signal from the virus. Image from verified customer review.](http://images.novusbio.com/fullsize/Flavivirus-group-antigen-Antibody-D1-4G2-4-15-4G2-Chimeric-Flow-Cytometry-NBP2-52666-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Detection of Zika virus. Immunofluorescence images of Vero cells infected with ZIKV after 30h infection as well as controls. Cells were fixed in 4% PFA for 30 min at RT. Primary antibody (Rabbit IgG) staining was performed in 0.3% Triton X-100 at 1:20 dilution overnight at 4C, detected using a Goat Anti-Rabbit IgG (1:500) for 1h at RT and counterstained with DAPI.](http://images.novusbio.com/fullsize/Flavivirus-group-antigen-Antibody-D1-4G2-4-15-4G2-Chimeric-Immunofluorescence-NBP2-52666-img0001.png)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52680-img0003.jpg "Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.")
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab). PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (NBP2-52680, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Flow-Cytometry-NBP2-52680-img0002.jpg)
![Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (NBP2-52680) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52680, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunofluorescence-NBP2-52680-img0001.jpg)
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free [NBP2-52680] - Abundance of two potential Hla receptors & of caveolin-1 as a potential pore-stabilizing factor.A: Representative examples of immune fluorescence assays using epifluorescence microscopy (nuclei counterstained using DAPI) that were performed on 16HBE14o-, S9 or A549 cells grown on coverslips using antibodies against ADAM10, the alpha 5 beta 1 integrin or against caveolin-1. Staining appearing in red represents specific labelling of the respective proteins. Scale bars: 10 μm. B: Semi-quantitative determination of primary & secondary antibody-mediated fluorescence in suspended individual cells by flow cytometry. During flow cytometry, the fluorescence of the antibody-tagged proteins per cell was measured & the respective medians of the detected peaks were used for calculating the means ± S.D. for the biological replicates (n = 4, each). Individual means were tested for significant differences using Student’s t-test or Welch’s t-test: * = p ≤ 0.05, ** = p ≤ 0.01 or *** = p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32470006), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-52680_rabbit-monoclonal-integrin-alpha-5-beta-1-antibody-m200-volociximab-chimeric-310202416114751.jpg)
![Immunocytochemistry/Immunofluorescence: Common beta Chain Antibody (BION-1) - Chimeric [NBP3-09025] - Human epithelial cells stained with NBP3-09025. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Common-beta-Chain-Antibody-BION-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09025-img0003.jpg "Immunocytochemistry/Immunofluorescence: Common beta Chain Antibody (BION-1) - Chimeric [NBP3-09025] - Human epithelial cells stained with NBP3-09025. ICC/IF image submitted by a verified customer review.")
![Flow Cytometry: Common beta Chain Antibody (BION-1) - Chimeric [NBP3-09025] - U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of BION-1 ((NBP3-09025, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Common-beta-Chain-Antibody-BION-1-Chimeric-Flow-Cytometry-NBP3-09025-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Common beta Chain Antibody (BION-1) - Chimeric [NBP3-09025] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of BION-1 (NBP3-09025) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09025, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Common-beta-Chain-Antibody-BION-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09025-img0002.jpg)
![Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0006.jpg "Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.")
![Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Formalin fixed human thyroid cancer slices were were stained with a NBP2-75896 at 5 ug/ml.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0003.jpg)
![Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0004.jpg)
![Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0005.jpg)
![Immunofluorescence: CD52 Antibody (Campath-1H) [NBP2-75896] - Formaldehyde-fixed rat thymus slices were stained with NBP2-75896 at 5 ug/ml and detected with a FITC-conjugated secondary antibody.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Immunofluorescence-NBP2-75896-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-CR3022-ELISA-NBP2-90980-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.")
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) [NBP2-90980] - Binding curve of SARS-CoV-2 Spike Antibody (CR3022) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag (blue line) or SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag (orange line) from HEK293 cells (Native Antigen) at concentrations of 5 ug/ml. A 3-fold serial dilution from 125 ng/ml was performed using SARS-CoV-2 Spike Antibody (CR3022). For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-CR3022-ELISA-NBP2-90980-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (CR3022) - Azide and BSA Free [NBP2-90980] - ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) at concentrations of 5 ug/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using NBP2-90980; from 370 ng/ml for NBP3-11813 and from 10000 ng/ml for NBP3-11776. Human IgM and human IgA were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.](http://images.novusbio.com/fullsize/nbp2-90980_human-monoclonal-sars-cov-2-spike-antibody-cr3022-7420252228562.png)
![Dual RNAscope ISH-IHC: CD52 Antibody (Campath-1H) [NBP2-52645] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD52 mRNA (ACD RNAScope probe, catalog # 519381; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using rabbit monoclonal (Novus catalog # NBP2-52645) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC003) and green HRP chromogen (ACD Catalog # 322500).](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Dual-RNAscope-ISH-IHC-NBP2-52645-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD52 Antibody (Campath-1H) [NBP2-52645] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD52 mRNA (ACD RNAScope probe, catalog # 519381; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using rabbit monoclonal (Novus catalog # NBP2-52645) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC003) and green HRP chromogen (ACD Catalog # 322500).")
![<b>Orthogonal Strategies Validation. </b>Flow Cytometry: CD52 Antibody (Campath-1H) [NBP2-52645] - Flow-cytometry using anti-CD52 antibody Campath-1H. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-NBP2-52645-img0005.jpg)
![Flow Cytometry: CD52 Antibody (Campath-1H) [NBP2-52645] - Flow-cytometry using anti-CD52 antibody Campath-1H. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-NBP2-52645-img0004.jpg)
![Simple Western: His Tag Antibody (3D5) - Chimeric [NBP2-52637] - His Tag Antibody (3D5) [NBP2-52637] - Human purified protein. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-3D5-Simple-Western-NBP2-52637-img0002.jpg "Simple Western: His Tag Antibody (3D5) - Chimeric [NBP2-52637] - His Tag Antibody (3D5) [NBP2-52637] - Human purified protein. Simple Western image submitted by a verified customer review.")
![Western Blot: His Tag Antibody (3D5) - Chimeric - Azide and BSA Free [NBP2-52637] - His tag expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3g protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP2-52637 at 0.01ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp2-52637_rabbit-monoclonal-his-tag-antibody-3d5-chimeric-74202522141228.png)
![Immunocytochemistry /Immunofluorescence: His Tag Antibody (3D5) - Chimeric - Azide and BSA Free [NBP2-52637] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with His tag expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with NBP2-52637 (1:200 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-52637_rabbit-monoclonal-his-tag-antibody-3d5-chimeric-74202522141211.png)