| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1188AF405 | |||
| Alexa Fluor 488 | NB21-1188AF488 | |||
| Alexa Fluor 647 | NB21-1188AF647 | |||
| Alexa Fluor 700 | NB21-1188AF700 | |||
| Biotin | NB21-1188B | |||
| DyLight 405 | NB21-1188V | |||
| DyLight 488 | NB21-1188G | |||
| DyLight 550 | NB21-1188R | |||
| DyLight 650 | NB21-1188C | |||
| FITC | NB21-1188F | |||
| HRP | NB21-1188H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1188SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56595SS | |||
| Azide and BSA Free | NBP2-33237 | |||
![Western Blot: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - Whole extract of HeLa cell treated with nocodazole was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. Antibody dilution 0.5 ug/mL2. Antibody dilution 1 ug/mL3. Antibody dilution 2 ug/mL](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Western-Blot-NB600-1168-img0008.jpg "Western Blot: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - Whole extract of HeLa cell treated with nocodazole was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. Antibody dilution 0.5 ug/mL2. Antibody dilution 1 ug/mL3. Antibody dilution 2 ug/mL")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 5 ug/mL Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG, FITC conjugate (green). Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Immunocytochemistry-Immunofluorescence-NB600-1168-img0007.jpg)
![Flow Cytometry: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - FACS profile of human leukemic cells with Anti-phospho-Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Flow-Cytometry-NB600-1168-img0006.jpg)
![Dot Blot: Histone H3 [p Ser28] Antibody [NB21-1188] - Dot blot analysis of Histone H3 pS28 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28]-Antibody-Dot-Blot-NB21-1188-img0003.jpg "Dot Blot: Histone H3 [p Ser28] Antibody [NB21-1188] - Dot blot analysis of Histone H3 pS28 in picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28] Antibody [NB21-1188] - Histone H3.2 pS28 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1188-img0007.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody [NB21-1188] - WB analysis of Histone H3 pS28 in NIH 3T3 histone prep. Observed molecular weight is ~14 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28]-Antibody-Western-Blot-NB21-1188-img0002.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody (117C826) [NB100-56595] - Analysis using Azide and BSA Free version of NB100-56595. Histone H3 phospho (Ser28) in (1) untreated, and (2) Fas antibody treated Jurkat lysate using Histone H3 phospho antibody at 2 ug/ml. Observed molecular weight is ~17 kDa](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-117C826-Western-Blot-NB100-56595-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Ser28] Antibody (117C826) [NB100-56595] - Analysis using Azide and BSA Free version of NB100-56595. Histone H3 phospho (Ser28) in (1) untreated, and (2) Fas antibody treated Jurkat lysate using Histone H3 phospho antibody at 2 ug/ml. Observed molecular weight is ~17 kDa")
![Western Blot: Histone H3 [p Ser28] Antibody (JE56-06) [NBP3-32771] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32771) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate Lane 3: HeLa treated with 100ng/mL Nocodazole for 18 hours,then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32771) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32771_rabbit-histone-h3-p-ser28-mab-je56-06-288202412442913.jpg "Western Blot: Histone H3 [p Ser28] Antibody (JE56-06) [NBP3-32771] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32771) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate Lane 3: HeLa treated with 100ng/mL Nocodazole for 18 hours,then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32771) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: p62/SQSTM1 [p Ser28] Antibody [NBP2-60272] - Analysis of Jurkat cell lysate showing specific immunolabeling of the 48 kDa p62 phosphorylated at Ser28 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).](http://images.novusbio.com/fullsize/p62-SQSTM1-[p-Ser28]-Antibody-Western-Blot-NBP2-60272-img0001.jpg "Western Blot: p62/SQSTM1 [p Ser28] Antibody [NBP2-60272] - Analysis of Jurkat cell lysate showing specific immunolabeling of the 48 kDa p62 phosphorylated at Ser28 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).")