Hu,
Mu,
Rt,
Bv,
Ch,
Ha,
Pm-OrSpecies Glossary
Reactivity: Hu, Mu, Rt, Bv, Ch, Ha, Pm-Or
WB, Simple Western, Flow, ICC/IF, IHC, WB, Mycoplasma
Applications: WB, Simple Western, Flow, ICC/IF, IHC, WB, Mycoplasma
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0015.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of extracts from HeLa and COS cells using NBP1-77397 Hsp60 antibody at 1:1000](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77397-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0019.jpg)
![Immunohistochemistry: HSP60 Antibody [NBP1-77397] - Staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunohistochemistry-NBP1-77397-img0006.jpg)
![Flow Cytometry: HSP60 Antibody [NBP1-77397] - Analysis of HSP60 in RM1 cells (murine prostate cancer cell line) using anti-HSP60 antibody. The primary antibody was used at a dilution of 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/HSP60-Antibody-Flow-Cytometry-NBP1-77397-img0014.jpg)
![Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of HSP60 in: 1) HeLa, 2) HepG2, 3) NIH/3T3, 4) Jurkat, 5) CHO, 6) A431, 7) PC12 and 8) COS7](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77397-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HSP60 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescence analysis of HeLa cells using Hsp60 antibody at 1:50 (green). Nuclei were counterstained using DAPI (blue).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody conjugated to Alexa Fluor 488 (NBP1-77397AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0018.jpg)
![Simple Western: HSP60 Antibody [NBP1-77397] - Lane view shows a specific band for Hsp60 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-Simple-Western-NBP1-77397-img0013.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0016.jpg "Western Blot: ATP5A Antibody [NBP2-15512] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: ATP5A Antibody [NBP2-15512] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:1000.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0004.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted by 1:2000.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0005.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0010.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0015.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Colon carcinoma. ATP5A1 antibody [C2C3], C-term dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse stomach. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse lung. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0007.jpg)
![ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (NBP2-15512) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15512_rabbit-polyclonal-atp5a-antibody-immunohistochemistry-frozen-26112025197457.jpg)
![ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (NBP2-15512) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15512_rabbit-polyclonal-atp5a-antibody-immunocytochemistry-immunofluorescence-2611202518384018.jpg)
![Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract12 % SDS-PAGE Prohibitin antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP2-19944-img0006.jpg "Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract12 % SDS-PAGE Prohibitin antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody [NBP2-19944] - SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37 c for 30 min.Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19944-img0007.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Paraffin-embedded mouse liver. Prohibitin antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunohistochemistry-Paraffin-NBP2-19944-img0004.jpg)
![Western Blot: Prohibitin Antibody [NBP2-19944] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP2-19944-img0001.jpg)
![Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extractC. 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP2-19944-img0005.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunohistochemistry-Paraffin-NBP2-19944-img0002.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Paraffin-embedded mouse intestine. Prohibitin antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunohistochemistry-Paraffin-NBP2-19944-img0003.jpg)
