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![Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NB100-1919-img0006.jpg "Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.")
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NB100-1919] - HeLa cells with antibody 1:500 dilution labeling mitochondria.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1919-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. The representative image shows a specific and expected granular staining of Mitofilin in the keratinocytes as well as the other cells of epidermal layer with no staining in basal cells. [60X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0009.jpg)
![Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import-defective. HEK293 cells that transiently expressed wild type or mutant COA7HIS were solubilized, and the affinity purification of COA7HIS was performed. The samples were analyzed by reducing and non-reducing SDS-PAGE and Western blot. Load: 3%. Eluate: 100%. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201809561), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NB100-1919-img0012.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P analysis of Mitofilin/Mitochondrial inner membrane protein in a transverse section of normal human skeletal muscle using Mitofilin antibody at 1:200 dilution. The myocytes showed an expected and very specific granular staining in the cytoplasm. [10X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0011.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. Specific cytoplasmic positivity of Mitofilin was observed in the keratinocytes as well as other cells of the epidermal layer except for basal cells and dermal connective tissue. [10X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0010.jpg)
![Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import‐defectiveASchematic representation of wild‐type & mutant COA7.BCellular protein extracts were isolated from HEK293 cells that were transfected with a plasmid that encoded wild‐type or mutant COA7. The samples were analyzed by reducing SDS–PAGE & Western blot.CCellular fractions were prepared from HEK293 cells that were transfected with a plasmid that encoded wild‐type or mutant COA7. The fractions were analyzed by reducing SDS–PAGE & Western blot. T, total; C, cytosol; M, mitochondria.DHEK293 cells that transiently expressed wild‐type or mutant COA7HIS were solubilized, & the affinity purification of COA7HIS was performed. The samples were analyzed by reducing & non‐reducing SDS–PAGE & Western blot. Load: 3%. Eluate: 100%.EEqual amounts of wild‐type & mutant radiolabeled [35S]COA7 precursors were imported into mitochondria isolated from HEK293 cells. The samples were analyzed by reducing SDS–PAGE & autoradiography. The results of three biological replicates were analyzed, quantified, & normalized to wild‐type COA7 at 45 min. The data are expressed as a mean ± SEM (n = 3). IAA, iodoacetamide.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30885959), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1919_rabbit-polyclonal-mitofilin-antibody-310202415532097.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Western-Blot-NBP2-21569-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cytochrome c Antibody [NBP2-21569] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human endometrial carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Immunohistochemistry-Paraffin-NBP2-21569-img0008.jpg)
![Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (30 ug of whole cell lysate) A: PC-12 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-C-Antibody-Western-Blot-NBP2-21569-img0002.jpg)
![Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-C-Antibody-Western-Blot-NBP2-21569-img0003.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Western-Blot-NBP2-21569-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human cervical carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Immunohistochemistry-Paraffin-NBP2-21569-img0007.jpg)
![Simple Western: Cytochrome c Antibody [NBP2-21569] - Cytochrome C Antibodies (NBP2-21569), ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the antibodies diluted 10 and 20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-simple-western-204202311476.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-91020242063356.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-91020242063396.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cytochrome c Antibody [NBP2-21569] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-1610202419405762.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0016.jpg "Western Blot: ATP5A Antibody [NBP2-15512] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: ATP5A Antibody [NBP2-15512] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:1000.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0004.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted by 1:2000.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0005.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0010.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0015.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Colon carcinoma. ATP5A1 antibody [C2C3], C-term dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse stomach. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse lung. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0007.jpg)
![ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (NBP2-15512) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15512_rabbit-polyclonal-atp5a-antibody-immunohistochemistry-frozen-26112025197457.jpg)
![ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (NBP2-15512) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15512_rabbit-polyclonal-atp5a-antibody-immunocytochemistry-immunofluorescence-2611202518384018.jpg)
![Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract12 % SDS-PAGE Prohibitin antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP2-19944-img0006.jpg "Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract12 % SDS-PAGE Prohibitin antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody [NBP2-19944] - SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37 c for 30 min.Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19944-img0007.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Paraffin-embedded mouse liver. Prohibitin antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunohistochemistry-Paraffin-NBP2-19944-img0004.jpg)
![Western Blot: Prohibitin Antibody [NBP2-19944] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP2-19944-img0001.jpg)
![Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extractC. 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP2-19944-img0005.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunohistochemistry-Paraffin-NBP2-19944-img0002.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Paraffin-embedded mouse intestine. Prohibitin antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunohistochemistry-Paraffin-NBP2-19944-img0003.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-68846-img0006.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-68846] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-68846-img0002.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A) and Mouse (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-68846-img0004.jpg)
![Western Blot: GRK2 Antibody [NB100-2831] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence. Approx 80 kDa band (calculated MW of 79.7 kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48 kDa and was successfully blocked by incubation with the immunizing peptide.](http://images.novusbio.com/fullsize/GRK2-Antibody-Western-Blot-NB100-2831-img0001.jpg "Western Blot: GRK2 Antibody [NB100-2831] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence. Approx 80 kDa band (calculated MW of 79.7 kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48 kDa and was successfully blocked by incubation with the immunizing peptide.")