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![Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-Western-Blot-NBP1-30027-img0013.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.")
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Angiotensinogen at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30027-img0014.jpg)
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-30027-img0012.jpg)
![Western Blot: Serpin A8/Angiotensinogen Antibody (001) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-(001)-Western-Blot-NBP2-89736-img0002.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody (001) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa")
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (001) [NBP2-89736] - Staining of Human AGT in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human AGT monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-(001)-Immunocytochemistry-Immunofluorescence-NBP2-89736-img0001.jpg)
![Flow Cytometry: Serpin A8/Angiotensinogen Antibody (001) [NBP2-89736] - Analysis of Human AGT expression on HepG2 cells. The cells were treated,stained with purified anti-Human AGT, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-(001)-Flow-Cytometry-NBP2-89736-img0004.jpg)
![Immunoprecipitation: Serpin A8/Angiotensinogen Antibody (001) [NBP2-89736] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SerpinA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SerpinA8 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa Observed band size: 51 kDa](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-(001)-Immunoprecipitation-NBP2-89736-img0003.jpg)
![Serpin A8/Angiotensinogen [Biotin]](http://images.novusbio.com/fullsize2/DATA_Serpin_A8_DY315605_ELISA_2426.jpg "Serpin A8/Angiotensinogen [Biotin]")
![Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-87053] - Analysis in control (vector only transfected HEK293T lysate) and AGT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/Serpin A8-Angiotensinogen Antibody-Western Blot-NBP1-87053-img0008.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-87053] - Analysis in control (vector only transfected HEK293T lysate) and AGT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-87053] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-87053-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (SD201-02) [NBP2-67778] - Staining Angiotensinogen in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-(SD201-02)-Immunocytochemistry-Immunofluorescence-NBP2-67778-img0003.jpg "Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (SD201-02) [NBP2-67778] - Staining Angiotensinogen in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (SD201-02) [NBP2-67778] - Staining Angiotensinogen in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-(SD201-02)-Immunocytochemistry-Immunofluorescence-NBP2-67778-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (SD201-02) [NBP2-67778] - Staining Angiotensinogen in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Serpin-A8-Angiotensinogen-Antibody-(SD201-02)-Immunocytochemistry-Immunofluorescence-NBP2-67778-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (SD201-02) [NBP2-67778] - Staining Angiotensinogen in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Serpin A8-Angiotensinogen Antibody (SD201-02)-Immunocytochemistry-Immunofluorescence-NBP2-67778-img0004.jpg)
![SDS-Page: Recombinant Human Serpin A8/Angiotensinogen Protein [H00000183-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize2/Recombinant-Human-Serpin-A8-Angiotensinogen-Protein-SDS-Page-H00000183-P01-img0001.jpg "SDS-Page: Recombinant Human Serpin A8/Angiotensinogen Protein [H00000183-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human Serpin A8/Angiotensinogen Protein [H00000183-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize2/Recombinant-Human-Serpin-A8-Angiotensinogen-Protein-SDS-Page-H00000183-Q01-img0001.jpg "SDS-Page: Recombinant Human Serpin A8/Angiotensinogen Protein [H00000183-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
-img0001.jpg "Rat Serpin A8/Angiotensinogen ELISA Kit (Chemiluminescence) - Standard Curve Reference")