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NBP1-97657

LAG-3 Antibody (17B4)

(2 Reviews)

(10 Publications)

50 μg

NBP1-97657

Western Blot: LAG-3 Antibody (17B4) [NBP1-97657] - Lane 1: MW marker. Lane 2: LAG-3 (human):Fc (human).

Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. Staining of LAG-3 on CD4+ and CD8+ subpopulations of tumour infiltrating lymphocytes (TILs) detected with LAG-3 (human), mAb (17B4) (FITC).

Immunohistochemistry-Paraffin: LAG-3 Antibody (17B4) [NBP1-97657] - LAG-3 in human tonsil tissue sample. Image submitted by a verified customer review.

Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. LAG-3 expression on activated human peripheral blood mononuclear cells (PBMC) detected with LAG-3 (human), mAb (17B4) (FITC).Method: T lymphocytes from human PBMC are stimulated with 1 ug/mL of PHA for three days. Then, after seven days of culture, 3x10^6 three-days PHA-activated human PBMC are treated with LAG-3 (human), mAb (17B4) (FITC) or FITC coupled isotype-matched (IgG1) control MAb (used at a saturating dilution of 1:800 and 1:150 respectively) for 30 min. at 4C in RPMI 1640 and washed twice with 1X PBS. Stained cells are then analysed by FC [4].

Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. Method: LAG-3 (human), mAb (17B4) (FITC) (10 ug/mL) (is preincubated with a specific peptide epitope (208b) or a control tetanus toxoid (TT) peptide at different molarities prior to staining of TILs.

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-97657AF350
Alexa Fluor 405	NBP1-97657AF405
Alexa Fluor 488	NBP1-97657AF488
Alexa Fluor 532	NBP1-97657AF532
Alexa Fluor 594	NBP1-97657AF594
Alexa Fluor 647	NBP1-97657AF647
Alexa Fluor 700	NBP1-97657AF700
Alexa Fluor 750	NBP1-97657AF750
Allophycocyanin	NBP1-97657APC
Allophycocyanin/Cy7	NBP1-97657APCCY7
Biotin	NBP1-97657B
DyLight 350	NBP1-97657UV
DyLight 405	NBP1-97657V
DyLight 488	NBP1-97657G
DyLight 550	NBP1-97657R
DyLight 594	NBP1-97657DL594
DyLight 650	NBP1-97657C
DyLight 680	NBP1-97657FR
DyLight 755	NBP1-97657IR
FITC	NBP1-97665
HRP	NBP1-97657H
Janelia Fluor 549	NBP1-97657JF549
Janelia Fluor 646	NBP1-97657JF646
PE	NBP1-97657PE
PE/Atto594	NBP1-97657PEATT594
PE/Cy5.5	NBP1-97657PECY55
PE/Cy7	NBP1-97657PECY7
PerCP	NBP1-97657PCP

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AF2319

LAG-3 Antibody [Unconjugated]

(5 Publications)

100 μg

AF2319

HEK293 human embryonic kidney cell line transfected with either (A) human LAG-3 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Human LAG-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2319) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). Quadrant markers were set based on control antibody staining(Catalog # AB-108-C, data not shown). View our protocol for Staining Membrane-associated Proteins.

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated or treated (+) with 1 ug/mL PHA for 5 days and HDLM‑2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LAG‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2319) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LAG‑3 at approximately 60-75 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 1 ug/mL PHA for 5 days were stained with Goat Anti-Human LAG‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2319) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) and Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Allophycocyanin	FAB2319A
Biotin	BAF2319
Fluorescein	FAB2319F
PerCP (Peridinin-chlorophyll Protein Complex)	FAB2319C
Phycoerythrin	FAB2319P

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB2319C
Carrier Free	FAB2319F
Carrier Free	FAB2319P
Carrier Free	FAB2319A
Carrier Free	AF2319-SP

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NBP1-85781

LAG-3 Antibody

(3 Publications)

0.1 ml

NBP1-85781

Immunohistochemistry-Paraffin: LAG-3 Antibody [NBP1-85781] - Staining of human cerebral cortex shows low expression as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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AF3328

LAG-3 Antibody [Unconjugated]

(1 Review)

(1 Publication)

100 μg

AF3328

Western blot shows lysates of mouse liver tissue, mouse heart tissue, EL4.IL‑2 mouse lymphoblast cell line, and CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LAG‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3328) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for LAG‑3 at approximately 54 kDa and 75 kDa in mouse liver and mouse heart tissue and 70-80 kDa in EL4.IL-2 and CTLL-2 cell lines (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3328-SP

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NBP2-76403

LAG-3 Antibody (BLR028F)

100 ul

NBP2-76403

Immunocytochemistry: LAG-3 Antibody (BLR028F) [NBP2-76403] - Detection of human LAG3 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR028F] (NBP2-76403) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: LAG-3 Antibody (BLR028F) [NBP2-76403] - Section of tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR028F] used at 1:200. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-97662

LAG-3 Antibody (11E3)

(1 Publication)

0.05 mg

NBP1-97662

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-97662AF350
Alexa Fluor 532	NBP1-97662AF532
Alexa Fluor 594	NBP1-97662AF594
Alexa Fluor 750	NBP1-97662AF750
Allophycocyanin	NBP1-97662APC-0.1ml
Allophycocyanin/Cy7	NBP1-97662APCCY7
Biotin	NBP1-97662B
DyLight 350	NBP1-97662UV-0.1ml
DyLight 405	NBP1-97662V-0.1ml
DyLight 488	NBP1-97662G-0.1ml
DyLight 550	NBP1-97662R
DyLight 594	NBP1-97662DL594
DyLight 650	NBP1-97662C
DyLight 680	NBP1-97662FR-0.1ml
DyLight 755	NBP1-97662IR-0.1ml
FITC	NBP1-97662F
HRP	NBP1-97662H
Janelia Fluor 549	NBP1-97662JF549
Janelia Fluor 646	NBP1-97662JF646
PE	NBP1-97662PE-0.1ml
PE/Atto594	NBP1-97662PEATT594
PE/Cy5.5	NBP1-97662PECY55
PE/Cy7	NBP1-97662PECY7

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NBP2-76402

LAG-3 Antibody (BLR027F)

100 ul

NBP2-76402

Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (BLR027F) [NBP2-76402] - Section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG.Substrate: Opal™. Counterstain: DAPI (blue).

Immunohistochemistry-Paraffin: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402). Secondary: HRP-conjugated goat anti-rabbit IgG. Substate: DAB.

Immunohistochemistry-Paraffin: LAG-3 Antibody (BLR027F) [NBP2-76402] - Detection of human LAG3 in FFPE tonsil by IHC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (NBP2-76402), Secondary: HRP-conjugated goat anti-rabbit IgG. Substate: DAB.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB23193

LAG-3 Antibody (874501) [Unconjugated]

100 μg

MAB23193

HEK293 human embryonic kidney cell line transfected with either (A) human LAG-3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23193) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood mononuclear cells (PBMCs) were either untreated (top panel) or treated with with 5 μg/mL PHA (bottom panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG‑3 Monoclonal Antibody (Catalog # MAB23193) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002) (data not shown). View our protocol for Staining Membrane-associated Proteins.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB23193U-100UG
Alexa Fluor 405	FAB23193V-100UG
Alexa Fluor 488	FAB23193G
Alexa Fluor 594	FAB23193T-100UG
Alexa Fluor 647	FAB23193R-100UG
Alexa Fluor 700	FAB23193N
Alexa Fluor 750	FAB23193S-100UG
Allophycocyanin	FAB23193A
Phycoerythrin	FAB23193P

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB23193A
Carrier Free	FAB23193G
Carrier Free	FAB23193N
Carrier Free	FAB23193P
Carrier Free	MAB23193-SP
Carrier Free	FAB23193V-100UG
Carrier Free	FAB23193U-100UG
Carrier Free	FAB23193T-100UG
Carrier Free	FAB23193S-100UG
Carrier Free	FAB23193R-100UG

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MAB23195

LAG-3 Antibody (1009611) [Unconjugated]

100 μg

MAB23195

Human peripheral blood mononuclear cells (PBMCs) were either untreated (bottom panel) or treated with with 5 μg/mL PHA (top panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23195) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003) (data not shown). View our protocol for Staining Membrane-associated Proteins.

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human LAG‑3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LAG‑3 Monoclonal Antibody (Catalog # MAB23195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LAG‑3 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB23195U-100UG
Alexa Fluor 405	FAB23195V-100UG
Alexa Fluor 488	FAB23195G-100UG
Alexa Fluor 594	FAB23195T-100UG
Alexa Fluor 647	FAB23195R-100UG
Alexa Fluor 700	FAB23195N-100UG
Alexa Fluor 750	FAB23195S-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB23195U-100UG
Carrier Free	FAB23195T-100UG
Carrier Free	FAB23195S-100UG
Carrier Free	FAB23195R-100UG
Carrier Free	FAB23195N-100UG
Carrier Free	FAB23195G-100UG
Carrier Free	FAB23195V-100UG
Carrier Free	MAB23195-SP

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MAB23194

LAG-3 Antibody (1009614) [Unconjugated]

100 μg

MAB23194

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB23194U-100UG
Alexa Fluor 405	FAB23194V-100UG
Alexa Fluor 488	FAB23194G-100UG
Alexa Fluor 594	FAB23194T-100UG
Alexa Fluor 647	FAB23194R-100UG
Alexa Fluor 700	FAB23194N-100UG
Alexa Fluor 750	FAB23194S-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB23194V-100UG
Carrier Free	FAB23194U-100UG
Carrier Free	FAB23194T-100UG
Carrier Free	FAB23194S-100UG
Carrier Free	FAB23194R-100UG
Carrier Free	FAB23194N-100UG
Carrier Free	FAB23194G-100UG
Carrier Free	MAB23194-SP

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MAB33281

LAG-3 Antibody (631501) [Unconjugated]

100 μg

MAB33281

Mouse splenocytes were treated for 72 hours with 50 ng/mL PMA and 500 ng/mL Ca2+ Ionomycin then stained with Rat Anti-MouseLAG‑3 Monoclonal Antibody (Catalog # MAB33281, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB33281U-100UG
Alexa Fluor 405	FAB33281V-100UG
Alexa Fluor 488	FAB33281G-100UG
Alexa Fluor 594	FAB33281T-100UG
Alexa Fluor 647	FAB33281R-100UG
Alexa Fluor 700	FAB33281N
Alexa Fluor 750	FAB33281S-100UG
Phycoerythrin	FAB33281P-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB33281N
Carrier Free	MAB33281-SP
Carrier Free	FAB33281V-100UG
Carrier Free	FAB33281U-100UG
Carrier Free	FAB33281T-100UG
Carrier Free	FAB33281S-100UG
Carrier Free	FAB33281R-100UG
Carrier Free	FAB33281P-100UG
Carrier Free	FAB33281G-100UG

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2319-L3

Recombinant Human LAG-3 Fc Chimera Protein, CF

(2 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Related Diseases

Gene Symbol

Images Type

Isotype

Related Pathways

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

LAG-3 Antibody (17B4)

LAG-3 Antibody [Unconjugated]

LAG-3 Antibody

LAG-3 Antibody [Unconjugated]

LAG-3 Antibody (BLR028F)

LAG-3 Antibody (11E3)

LAG-3 Antibody (BLR027F)

LAG-3 Antibody (874501) [Unconjugated]

LAG-3 Antibody (1009611) [Unconjugated]

LAG-3 Antibody (1009614) [Unconjugated]

LAG-3 Antibody (631501) [Unconjugated]

Recombinant Human LAG-3 Fc Chimera Protein, CF