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![Western Blot: Mouse IgG Fc Isotype Control [NBP1-96784] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize2/Mouse-IgG-Fc-Isotype-Control-Western-Blot-NBP1-96784-img0006.jpg "Western Blot: Mouse IgG Fc Isotype Control [NBP1-96784] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![SDS-Page: Mouse IgG Fc Isotype Control [NBP1-96784] - SDS-PAGE of Mouse IgG F(c) Fragment. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize2/Mouse-IgG-Fc-Isotype-Control-SDS-Page-NBP1-96784-img0007.jpg)
![ELISA: Human IgG Fc ELISA Kit (Colorimetric) [NBP2-60068]](http://images.novusbio.com/fullsize2/Human-IgG-Fc-ELISA-Kit-(Colorimetric)-ELISA-NBP2-60068-img0001.jpg "ELISA: Human IgG Fc ELISA Kit (Colorimetric) [NBP2-60068]")
![SDS-Page: Human IgG Fc Isotype Control [NBP1-96973] - SDS-PAGE results of Human IgG F(c) Fragment. Lane 1: reduced Human IgG F(c) Fragment. Lane 2: Opal Prestained Molecular Weight Ladder. Lane 3: non-reduced Human IgG F(c) Fragment. Load: 1ug. 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.](http://images.novusbio.com/fullsize2/Human-IgG-Fc-Isotype-Control-SDS-Page-NBP1-96973-img0004.jpg "SDS-Page: Human IgG Fc Isotype Control [NBP1-96973] - SDS-PAGE results of Human IgG F(c) Fragment. Lane 1: reduced Human IgG F(c) Fragment. Lane 2: Opal Prestained Molecular Weight Ladder. Lane 3: non-reduced Human IgG F(c) Fragment. Load: 1ug. 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.")
![SDS-Page: Human IgG Fc Isotype Control [NBP1-96973] - SDS-PAGE of Human IgG Fc. Lane 1: Non-reduced Human IgG Fc Lane 2: Non-reduced Human IgG Whole Molecule Lane 3: Non-reduced Human IgG Fab Fragment Lane 4: Non-reduced Human IgG F(ab')2 Fragment. Middle Lane: 5uL OPAL Pre-stained Marker MB-210-0500. Lane 5: Reduced Human IgG Fc Lane 6: Reduced Human IgG Whole Molecule Lane 7: Reduced Human IgG Fab Fragment Lane 8: Reduced Human IgG F(ab')2 Fragment. Load: 1ug per lane. Human IgG Whole Molecule, Human IgG Fab Fragment and Human IgG F(ab')2 Fragment ran as controls. Predicted/Observed size: Non-reduced at 50 kDa, reduced at 25 kDa/Non-reduced at 55-60 kDa, reduced at 30-33 kDa.](http://images.novusbio.com/fullsize2/Human-IgG-Fc-Isotype-Control-SDS-Page-NBP1-96973-img0003.jpg)
![Dot Blot: Human IgG Fc Isotype Control [NBP1-96973] - Dot Blot: Human IgG Fc Isotype Control [Texas Red] [NBP1-97276] - Texas Red Conjugated Human IgG F(c). Load: 3-fold serial dilution starting at 200 ng. Image using the Texas Red form of this antibody.](http://images.novusbio.com/fullsize2/Human-IgG-Fc-Isotype-Control-Dot-Blot-NBP1-96973-img0005.jpg)
![Western Blot: IgG Fc Antibody (RMG02) [Unconjugated] [NBP2-62015] - Western blot of nonreduced(-) and reduced(+) rabbit IgG, using NBP2-62015. This antibody reacts to nonreduced rabbit IgG (~150 kDa).](http://images.novusbio.com/fullsize2/IgG-Fc-Antibody-(RMG02)-[Unconjugated]-Western-Blot-NBP2-62015-img0004.jpg "Western Blot: IgG Fc Antibody (RMG02) [Unconjugated] [NBP2-62015] - Western blot of nonreduced(-) and reduced(+) rabbit IgG, using NBP2-62015. This antibody reacts to nonreduced rabbit IgG (~150 kDa).")
![ELISA: IgG Fc Antibody (RMG02) [Unconjugated] [NBP2-62015] - A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of NBP2-62015 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.](http://images.novusbio.com/fullsize2/IgG Fc Antibody (RMG02) [Unconjugated]-ELISA-NBP2-62015-img0006.jpg)
![ELISA: IgG Fc Antibody (RMG02) [Unconjugated] [NBP2-62015] - ELISA of IgGs from different species shows NBP2-62015 reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with different IgGs. NBP2-62015 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.](http://images.novusbio.com/fullsize2/IgG-Fc-Antibody-(RMG02)-[Unconjugated]-ELISA-NBP2-62015-img0005.jpg)
![SDS-Page: Rat IgG Fc Isotype Control [NBP1-96967] - SDS-PAGE of Rat IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c) and Fab; 1.5 ug of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize2/Rat-IgG-Fc-Isotype-Control-SDS-Page-NBP1-96967-img0003.jpg "SDS-Page: Rat IgG Fc Isotype Control [NBP1-96967] - SDS-PAGE of Rat IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c) and Fab; 1.5 ug of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.")
![SDS-Page: Rat IgG Fc Isotype Control [NBP1-96967] - SDS-Page of Rat IgG F(c). Lane 1: Rat Fc - Non-reduced. Lane 2: Rat Fc - Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 25 kDa, 25 kDa for Reduced Fc. Other band(s): None.](http://images.novusbio.com/fullsize2/Rat-IgG-Fc-Isotype-Control-SDS-Page-NBP1-96967-img0005.jpg)
![SDS-Page: Goat IgG Fc Isotype Control [NBP1-97042] - SDS-PAGE of Goat IgG F(c) Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule. Lane 2: Reduced Goat IgG F(c) Fragment. Lane 3: Reduced Goat IgG F(ab) Fragment. Lane 4: Reduced Goat IgM Whole Molecule. Load: 1 ug for IgG, F(c) and F(ab); 3 ug for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize2/Goat-IgG-Fc-Isotype-Control-SDS-Page-NBP1-97042-img0006.jpg "SDS-Page: Goat IgG Fc Isotype Control [NBP1-97042] - SDS-PAGE of Goat IgG F(c) Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule. Lane 2: Reduced Goat IgG F(c) Fragment. Lane 3: Reduced Goat IgG F(ab) Fragment. Lane 4: Reduced Goat IgM Whole Molecule. Load: 1 ug for IgG, F(c) and F(ab); 3 ug for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.")
![Dot Blot: Goat IgG Fc Isotype Control [NBP1-97042] - Dot Blot: Goat IgG Fc Isotype Control [Rhodamine] [NBP1-96842] - Dot Blot of Rhodamine Conjugated Goat IgG Fc. Dotted Rhodamine Conjugated Goat IgG Fc with following concentrations. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng Image using the Rhodamine form of this antibody.](http://images.novusbio.com/fullsize2/Goat-IgG-Fc-Isotype-Control-Dot-Blot-NBP1-97042-img0007.jpg)
![ELISA: IgG Fc Antibody (RMG06) [Unconjugated] [NBP2-62017] - A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of NBP2-62017 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.](http://images.novusbio.com/fullsize2/IgG Fc Antibody (RMG06) [Unconjugated]-ELISA-NBP2-62017-img0004.jpg "ELISA: IgG Fc Antibody (RMG06) [Unconjugated] [NBP2-62017] - A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of NBP2-62017 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.")
![ELISA: IgG Fc Antibody (RMG06) [Unconjugated] [NBP2-62017] - ELISA of mouse immunoglobulins shows NBP2-62017 reacts to the Fc region of mouse IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with different immunoglobulins. NBP2-62017 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.](http://images.novusbio.com/fullsize2/IgG-Fc-Antibody-(RMG06)-[Unconjugated]-ELISA-NBP2-62017-img0003.jpg)
![SDS-Page: Chicken IgG, Fc Native Protein [NBP1-96786] - SDS-PAGE of Chicken IgG F(c) Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.](http://images.novusbio.com/fullsize2/Chicken IgG- Fc Native Protein-SDS-Page-NBP1-96786-img0002.jpg "SDS-Page: Chicken IgG, Fc Native Protein [NBP1-96786] - SDS-PAGE of Chicken IgG F(c) Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.")
![SDS-Page: Rabbit IgG Fc Isotype Control [Biotin] [NBP1-97231] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment Biotin Conjugated. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.](http://images.novusbio.com/fullsize2/Rabbit-IgG-Fc-Isotype-Control-[Biotin]-SDS-Page-NBP1-97231-img0001.jpg "SDS-Page: Rabbit IgG Fc Isotype Control [Biotin] [NBP1-97231] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment Biotin Conjugated. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.")