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![SDS-Page: Human IgG, Fab Native Protein [NBP1-97020] - SDS-PAGE results of Human IgG Fab Fragment. Lane 1: reduced Human IgG Fab Fragment (5ug). Lane 2: reduced Human IgG Fab Fragment (1ug). Lane 3: Opal Prestained Molecular Weight Ladder. Lane 4: non-reduced Human IgG Fab Fragment (1ug). Lane 5: non-reduced Human IgG Fab Fragment (5ug). 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.](http://images.novusbio.com/fullsize2/Human IgG- Fab Native Protein-SDS-Page-NBP1-97020-img0005.jpg "SDS-Page: Human IgG, Fab Native Protein [NBP1-97020] - SDS-PAGE results of Human IgG Fab Fragment. Lane 1: reduced Human IgG Fab Fragment (5ug). Lane 2: reduced Human IgG Fab Fragment (1ug). Lane 3: Opal Prestained Molecular Weight Ladder. Lane 4: non-reduced Human IgG Fab Fragment (1ug). Lane 5: non-reduced Human IgG Fab Fragment (5ug). 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.")
![SDS-Page: Human IgG, Fab Native Protein [NBP1-97020] - SDS-Page of Human Fab. Lane 1: Human Fab - Non-Reduced. Lane 2: Human Fab - Reduced. Load: 1.0 ug per lane. Predicted/Observed size - Non-Reduced: 50 kDa, 50 kDa for Human Fab. Predicted/Observed size - Reduced: 25 kDa, 25 kDa for Human Fab. Other band(s): None.](http://images.novusbio.com/fullsize2/Human-IgG--Fab-Native-Protein-SDS-Page-NBP1-97020-img0004.jpg)
![Western Blot: Rabbit IgG F(ab) Isotype Control [NBP1-97016] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize2/Rabbit-IgG-F(ab)-Isotype-Control-Western-Blot-NBP1-97016-img0006.jpg "Western Blot: Rabbit IgG F(ab) Isotype Control [NBP1-97016] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![SDS-Page: Rabbit IgG F(ab) Isotype Control [NBP1-97016] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment (BULK ORDER). Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.](http://images.novusbio.com/fullsize2/Rabbit-IgG-F(ab)-Isotype-Control-SDS-Page-NBP1-97016-img0004.jpg)
![Western Blot: IgG F(ab) Antibody (RMG01) [Unconjugated] [NBP2-62014] - Western blot of nonreduced(-) and reduced(+) rabbit IgG, using NBP2-62014. This antibody reacts to nonreduced rabbit IgG (~150 kDa).](http://images.novusbio.com/fullsize2/IgG-F(ab)-Antibody-(RMG01)-[Unconjugated]-Western-Blot-NBP2-62014-img0004.jpg "Western Blot: IgG F(ab) Antibody (RMG01) [Unconjugated] [NBP2-62014] - Western blot of nonreduced(-) and reduced(+) rabbit IgG, using NBP2-62014. This antibody reacts to nonreduced rabbit IgG (~150 kDa).")
![ELISA: IgG F(ab) Antibody (RMG01) [Unconjugated] [NBP2-62014] - A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of NBP2-62014 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.](http://images.novusbio.com/fullsize2/IgG F(ab) Antibody (RMG01) [Unconjugated]-ELISA-NBP2-62014-img0006.jpg)
![ELISA: IgG F(ab) Antibody (RMG01) [Unconjugated] [NBP2-62014] - ELISA of IgGs from different species, shows NBP2-62014 reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with different IgGs. NBP2-62014 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.](http://images.novusbio.com/fullsize2/IgG-F(ab)-Antibody-(RMG01)-[Unconjugated]-ELISA-NBP2-62014-img0005.jpg)
![Western Blot: Mouse anti-Human IgG F(ab) Secondary Antibody (4A11) [NB500-467] - Fig. 1. Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-human IgG Fab fragment (4A11). Lane 1: Normal human immunoglobulins Lane 2: Normal bovine immunoglobulins.](http://images.novusbio.com/fullsize2/Mouse-anti-Human-IgG-Secondary-Antibody-(4A11)-Western-Blot-NB500-467-img0001.jpg "Western Blot: Mouse anti-Human IgG F(ab) Secondary Antibody (4A11) [NB500-467] - Fig. 1. Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-human IgG Fab fragment (4A11). Lane 1: Normal human immunoglobulins Lane 2: Normal bovine immunoglobulins.")
![Flow Cytometry: Mouse anti-Human IgG F(ab) Secondary Antibody (4A11) [NB500-467] - Surface staining of human peripheral blood cells with anti-human IgG Fab fragment (4A11) PE-CyTM7.](http://images.novusbio.com/fullsize2/Mouse-anti-Human-IgG-Secondary-Antibody-(4A11)-Flow-Cytometry-NB500-467-img0004.jpg)
![Flow Cytometry: Mouse anti-Human IgG F(ab) Secondary Antibody (4A11) [NB500-467] - Surface staining of human peripheral blood cells with anti-human IgG Fab fragment (4A11) PE.](http://images.novusbio.com/fullsize2/Mouse-anti-Human-IgG-Secondary-Antibody-(4A11)-Flow-Cytometry-NB500-467-img0002.jpg)
![Flow Cytometry: Mouse anti-Human IgG F(ab) Secondary Antibody (4A11) [NB500-467] - Surface staining of human peripheral blood cells with anti-human IgG Fab fragment (4A11) purified / GAM-APC.](http://images.novusbio.com/fullsize2/Mouse-anti-Human-IgG-Secondary-Antibody-(4A11)-Flow-Cytometry-NB500-467-img0003.jpg)
![ELISA: IgG F(ab) Antibody (RMG05) [Unconjugated] [NBP2-62016] - A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of NBP2-62016 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.](http://images.novusbio.com/fullsize2/IgG F(ab) Antibody (RMG05) [Unconjugated]-ELISA-NBP2-62016-img0004.jpg "ELISA: IgG F(ab) Antibody (RMG05) [Unconjugated] [NBP2-62016] - A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of NBP2-62016 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.")
![ELISA: IgG F(ab) Antibody (RMG05) [Unconjugated] [NBP2-62016] - ELISA of mouse immunoglobulins shows NBP2-62016 reacts to the Fab region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with different immunoglobulins. NBP2-62016 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.](http://images.novusbio.com/fullsize2/IgG-F(ab)-Antibody-(RMG05)-[Unconjugated]-ELISA-NBP2-62016-img0003.jpg)
![SDS-Page: Chicken IgG F(ab) Isotype Control [NBP1-96773] - SDS-PAGE of Chicken IgG Fab Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.](http://images.novusbio.com/fullsize2/Chicken-IgG-F(ab)-Isotype-Control-SDS-Page-NBP1-96773-img0002.jpg "SDS-Page: Chicken IgG F(ab) Isotype Control [NBP1-96773] - SDS-PAGE of Chicken IgG Fab Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.")