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=
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ArHa
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=
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=
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=
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GP
=
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=
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Note: Mouseover a species abbreviation on the product page to display the fullname.
Products (42)
Full Site
page
of
4
42 Results for "novus"
List
Grid
NB100-105
HIF-1 alpha Antibody (H1alpha67)
(33 Reviews)
(745 Publications)
0.1 ml
NB100-105
Reactivity:
Hu
,
Mu
,
Rt
,
Po
,
Bv
,
Ft
,
Mk
,
Pm
,
Rb
,
Sh
,
Xp
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Po
,
Bv
,
Ft
,
Mk
,
Pm
,
Rb
,
Sh
,
Xp
Applications:
WB
,
ChIP
,
ELISA
,
Flow
,
GS
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
,
LA
,
PLA
,
TCS
,
KO
Applications:
WB
,
ChIP
,
ELISA
,
Flow
,
GS
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
,
LA
,
PLA
,
TCS
,
KO
Host:
Mouse Monoclonal
Host:
Mouse Monoclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
Available Conjugates:
Validated by:
Validated by:
Conjugates
Conjugate
Catalog #
Availability
Size
Price
Alexa Fluor 405
NB100-105AF405
Alexa Fluor 488
NB100-105AF488
Alexa Fluor 647
NB100-105AF647
Alexa Fluor 700
NB100-105AF700
Allophycocyanin
NB100-105APC
Biotin
NB100-105B
DyLight 350
NB100-105UV
DyLight 405
NB100-105V
DyLight 488
NB100-105G
DyLight 550
NB100-105R
DyLight 650
NB100-105C
DyLight 680
NB100-105FR
DyLight 755
NB100-105IR
FITC
NB100-105F
HRP
NB100-105H
Janelia Fluor 549
NB100-105JF549
Janelia Fluor 646
NB100-105JF646
PE
NB100-105PE
PerCP
NB100-105PCP
compare
(select up to 3 products from this page to compare)
NB100-479
HIF-1 alpha Antibody
(21 Reviews)
(278 Publications)
0.1 ml
NB100-479
Reactivity:
Hu
,
Mu
,
Rt
,
Fi
,
Ha
,
Pm
,
Rb
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Fi
,
Ha
,
Pm
,
Rb
Applications:
WB
,
ChIP
,
Flow
,
IB
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
Applications:
WB
,
ChIP
,
Flow
,
IB
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
Available Conjugates:
Validated by:
Validated by:
Conjugates
Conjugate
Catalog #
Availability
Size
Price
Alexa Fluor 405
NB100-479AF405
Alexa Fluor 488
NB100-479AF488
Alexa Fluor 647
NB100-479AF647
Alexa Fluor 700
NB100-479AF700
Biotin
NB100-479B
DyLight 405
NB100-479V
DyLight 488
NB100-479G
DyLight 550
NB100-479R
DyLight 650
NB100-479C
FITC
NB100-479F
HRP
NB100-479H
Janelia Fluor 549
NB100-479JF549
Janelia Fluor 646
NB100-479JF646
compare
(select up to 3 products from this page to compare)
NB100-449
HIF-1 alpha Antibody
(26 Reviews)
(196 Publications)
0.1 ml
NB100-449
Reactivity:
Hu
,
Mu
,
Rt
,
Ca
,
Ch
,
Gt
,
Mk
,
Pm
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Ca
,
Ch
,
Gt
,
Mk
,
Pm
Applications:
WB
,
Simple Western
,
ChIP
,
ELISA
,
Flow
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
,
ICC/IF
Applications:
WB
,
Simple Western
,
ChIP
,
ELISA
,
Flow
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
,
ICC/IF
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Conjugate:
Unconjugated
Validated by:
Validated by:
compare
(select up to 3 products from this page to compare)
NB100-134
HIF-1 alpha Antibody
(13 Reviews)
(139 Publications)
0.1 ml
NB100-134
Reactivity:
Hu
,
Mu
,
Rt
,
Ca
,
GP
,
Pm
,
Xp
,
Ze
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Ca
,
GP
,
Pm
,
Xp
,
Ze
Applications:
WB
,
Simple Western
,
GS
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
,
KO
Applications:
WB
,
Simple Western
,
GS
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
,
KO
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
Available Conjugates:
Validated by:
Validated by:
Conjugates
Conjugate
Catalog #
Availability
Size
Price
Alexa Fluor 405
NB100-134AF405
Alexa Fluor 488
NB100-134AF488
Alexa Fluor 647
NB100-134AF647
Alexa Fluor 700
NB100-134AF700
Biotin
NB100-134B
DyLight 405
NB100-134V
DyLight 488
NB100-134G
DyLight 550
NB100-134R
DyLight 650
NB100-134C
FITC
NB100-134F
HRP
NB100-134H
Janelia Fluor 549
NB100-134JF549
Janelia Fluor 646
NB100-134JF646
compare
(select up to 3 products from this page to compare)
NB100-123
HIF-1 alpha Antibody (H1alpha67)
(7 Reviews)
(135 Publications)
0.1 ml
NB100-123
Reactivity:
Hu
,
Mu
,
Rt
,
Po
,
Av
,
Bv
,
Ca
,
Ft
,
Pm
,
Sh
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Po
,
Av
,
Bv
,
Ca
,
Ft
,
Pm
,
Sh
Applications:
WB
,
ChIP
,
ELISA
,
Flow
,
GS
,
ICC/IF
,
IHC
,
IHC-P
,
IP
,
KO
Applications:
WB
,
ChIP
,
ELISA
,
Flow
,
GS
,
ICC/IF
,
IHC
,
IHC-P
,
IP
,
KO
Host:
Mouse Monoclonal
Host:
Mouse Monoclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
Available Conjugates:
Validated by:
Validated by:
Conjugates
Conjugate
Catalog #
Availability
Size
Price
Alexa Fluor 405
NB100-123AF405
Alexa Fluor 488
NB100-123AF488
Alexa Fluor 647
NB100-123AF647
Alexa Fluor 700
NB100-123AF700
Allophycocyanin
NB100-123APC
Biotin
NB100-123B
DyLight 350
NB100-123UV
DyLight 405
NB100-123V
DyLight 488
NB100-123G
DyLight 550
NB100-123R
DyLight 650
NB100-123C
DyLight 680
NB100-123FR
DyLight 755
NB100-123IR
FITC
NB100-123F
HRP
NB100-123H
HiLyte Fluor 488
NB100-123L
PE
NB100-123PE
PerCP
NB100-123PCP
compare
(select up to 3 products from this page to compare)
NB100-131
HIF-1 alpha Antibody (ESEE122)
(3 Reviews)
(71 Publications)
0.1 ml
NB100-131
Reactivity:
Hu
,
Mu
,
Rt
,
Bv
,
Ca
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Bv
,
Ca
Applications:
WB
,
Simple Western
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
Applications:
WB
,
Simple Western
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
Host:
Mouse Monoclonal
Host:
Mouse Monoclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
Available Conjugates:
Validated by:
Validated by:
Conjugates
Conjugate
Catalog #
Availability
Size
Price
Alexa Fluor 405
NB100-131AF405
Alexa Fluor 488
NB100-131AF488
Alexa Fluor 647
NB100-131AF647
Alexa Fluor 700
NB100-131AF700
Biotin
NB100-131B
DyLight 350
NB100-131UV
DyLight 405
NB100-131V
DyLight 488
NB100-131G
DyLight 550
NB100-131R
DyLight 650
NB100-131C
DyLight 680
NB100-131FR
DyLight 755
NB100-131IR
FITC
NB100-131F
HRP
NB100-131H
Janelia Fluor 549
NB100-131JF549
Janelia Fluor 646
NB100-131JF646
compare
(select up to 3 products from this page to compare)
NB100-900WB
HIF-1 alpha Antibody Pack
(36 Publications)
5 Vials
NB100-900WB
Reactivity:
Hu
,
Mu
,
Rt
,
Pm
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Pm
Applications:
WB
,
ChIP
,
Flow
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
Applications:
WB
,
ChIP
,
Flow
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
Host:
Mouse
Host:
Mouse
compare
(select up to 3 products from this page to compare)
AF1935
HIF-1 alpha Antibody [Unconjugated]
(2 Reviews)
(17 Publications)
100 μg
AF1935
Reactivity:
Hu
,
Mu
,
Rt
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
Applications:
WB
,
Simple Western
,
IP
,
ChIP
,
ICC/IF
,
KO
Applications:
WB
,
Simple Western
,
IP
,
ChIP
,
ICC/IF
,
KO
Host:
Goat Polyclonal
Host:
Goat Polyclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
Validated by:
Validated by:
Available Formulations
Formulations
Formulation
Catalog #
Availability
Price
Carrier Free
AF1935-SP
compare
(select up to 3 products from this page to compare)
MAB1536
HIF-1 alpha Antibody (241809) [Unconjugated]
(25 Publications)
100 μg
MAB1536
Reactivity:
Hu
,
Mu
,
Rt
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
Applications:
WB
,
Simple Western
,
IP
,
KO
Applications:
WB
,
Simple Western
,
IP
,
KO
Host:
Mouse Monoclonal
Host:
Mouse Monoclonal
Conjugate:
Unconjugated
Available Formulations
Formulations
Formulation
Catalog #
Availability
Price
Carrier Free
MAB1536-SP
compare
(select up to 3 products from this page to compare)
NB100-654
HIF-1 alpha Antibody
(1 Review)
(9 Publications)
0.1 ml
NB100-654
Reactivity:
Hu
,
Mu
,
Rt
,
Po
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Po
Applications:
WB
,
Simple Western
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
Applications:
WB
,
Simple Western
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
Available Conjugates:
Validated by:
Validated by:
Conjugates
Conjugate
Catalog #
Availability
Size
Price
Alexa Fluor 405
NB100-654AF405
Alexa Fluor 488
NB100-654AF488
Alexa Fluor 647
NB100-654AF647
Alexa Fluor 700
NB100-654AF700
Biotin
NB100-654B
DyLight 405
NB100-654V
DyLight 488
NB100-654G
DyLight 550
NB100-654R
DyLight 650
NB100-654C
FITC
NB100-654F
HRP
NB100-654H
Janelia Fluor 549
NB100-654JF549
Janelia Fluor 646
NB100-654JF646
compare
(select up to 3 products from this page to compare)
NB100-905
HIF-1 alpha Antibody Pack
(2 Reviews)
(4 Publications)
5 Vials
NB100-905
Reactivity:
Hu
,
Mu
Species Glossary
Reactivity:
Hu
,
Mu
Applications:
WB
,
ChIP
,
Flow
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
Applications:
WB
,
ChIP
,
Flow
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
IP
compare
(select up to 3 products from this page to compare)
NB110-74679
HIF-1 alpha [Hydroxy Pro564] Antibody
(1 Review)
(7 Publications)
0.1 ml
NB110-74679
Reactivity:
Hu
,
Mu
,
Rt
,
Bv
,
Ca
,
Mk
,
Xp
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
,
Bv
,
Ca
,
Mk
,
Xp
Applications:
WB
,
ELISA
,
IB
,
IHC
,
IHC-P
Applications:
WB
,
ELISA
,
IB
,
IHC
,
IHC-P
Host:
Rabbit Polyclonal
Host:
Rabbit Polyclonal
Sizes:
Sample Size Available
Sizes:
Sample Size Available
Conjugate:
Unconjugated
compare
(select up to 3 products from this page to compare)
page
of
4
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-105-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Knockout-Validated-NB100-105-img0027.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-105-img0022.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis of HIF-1 alpha in human hepatocytes from cancer patient using anti-HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-105-img0021.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunohistochemistry-NB100-105-img0004.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Biotin conjugate of NB100-105. Staining of human glioblastoma multiforme.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunohistochemistry-Paraffin-NB100-105-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Expression of HIF-1 in intresititial pig cells under 12% of 02 condition. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunocytochemistry-Immunofluorescence-NB100-105-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1a was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFOA using 1 ug/mL of mouse anti-HIF-1a monoclonal (NB100-105, Novus Biologicals). Cells were stained using donkey anti-rabbit and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunocytochemistry-Immunofluorescence-NB100-105-img0023.jpg)
![Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in H929 cells using HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Flow-Cytometry-NB100-105-img0016.jpg)
![Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1a antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Flow-Cytometry-NB100-105-img0017.jpg)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Figure Legend: Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1a-deficient (Hif1a KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1a and Lamin B1 levels were analyzed by Western blot. This image was submitted via customer Review. (Mouse, WB)](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-105-img0026.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-479-img0016.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.")
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis of HIF-1 alpha in mouse hepatocytes (left lane: normoxic and right lane: hypoxic) using HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-479-img0018.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-479-img0017.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody [NB100-479] - Staining of human placenta, villi.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-NB100-479-img0019.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Staining in normal mouse kidney showing (A) staining with antibody and (B) blocking peptide used at 10-fold excess concentration. Images provided by Dr Yves Heremans. Please see the product review of NBC1-18422 for additional details.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-479-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-479] - Detection of HIF-1 Alpha (green) in RCC4 cells using NB100-479. Nuclei (Blue) were counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-479-img0011.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Analysis in paraffin section of mouse skin wound using NB100-479 at 1:6,000. Image courtesy of product review by Ian Darby of RMIT University.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-479-img0012.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-479] - Comparison of HIF-1 alpha levels in HMC-1.2 cells and CBMC cells under normoxic and hopoxic conditions. DFX was used as a positive control. [PMID: 20808808] Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-479-img0021.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-479] - U251 cells exposed to 4 hour hypoxia (0.5% oxygen) incubation. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-479-img0020.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody [NB100-479] - Analysis in normal mouse kidney. Image courtesy of product review by Yves Heremans.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-NB100-479-img0014.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Analysis in human kidney tumor.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-479-img0015.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-449] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-449] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Simple Western: HIF-1 alpha Antibody [NB100-449] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Simple-Western-NB100-449-img0032.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of mouse HIF1-alpha on hypoxia treated MEFs](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0037.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic).](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0036.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of Human HIF1 alpha by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells that were either treated with cobalt chloride (+; 200 mcM) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HIF1 alpha was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0038.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - Formaldehyde-fixed asynchronous HeLa cells.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-449-img0006.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-449] - Western blotting analyzed total proteins using anti-HIF-1 alpha, -ISCU, -FXN antibodies. CoCl2 was used to treat or not HeLa cells for 2 days. Citation: Ferecatu I, Canal F, Fabbri L, Mazure NM, Bouton C, Golinelli-Cohen M-P (2018) Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1 alpha activation. PLoS ONE 13(3): e0194782.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0045.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Mouse Brain, Neurons 40X](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-NB100-449-img0034.jpg)
![Flow Cytometry: HIF-1 alpha Antibody [NB100-449] - Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.125ug anti- HIF-alpha and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Red- untreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Flow-Cytometry-NB100-449-img0035.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-449] - Homogenate from pig (lanes 1 and 2) or rabbit (lane 4) aorta or lysate from cultured rat aortic smooth muscle cells (lane 3). Antibody: Affinity purified rabbit anti-SERCA2 used at 1 ug/ml (lanes 2 and 4) or 0.4 ug/ml (lane 3) for WB and 2 ug/mg lysate for IP or control (ctrl) monoclonal anti-SERCA2 (lanes 1 and 4) used at 1 ug/ml for WB. Detection: Chemiluminescence.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-449] - Analysis of HIF-1 alpha in human myeloma cell lysate using anti-HIF-1 alpha. Cells were untreated or treated with IGF-1, IL-6 or CoCl2. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0033.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of Human HIF1 alpha by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells that were treated with cobalt chloride (+; 200 mcM) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody NB100-449 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0042.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-449] - BMDM were seeded at 0.5x10^6 overnight. Cells were treated with 10 ng/ml LPS for 24 hrs, and a western blot was performed. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0043.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-449] - Western blot analyzed total proteins using anti-HIF-1 alpha, -CAIX, -ISCU antibodies and VDACs poly antibody. Citation: Ferecatu I, Canal F, Fabbri L, Mazure NM, Bouton C, Golinelli-Cohen M-P (2018) Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1 alpha activation. PLoS ONE 13(3): e0194782.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0044.jpg)
![Immunoprecipitation: HIF-1 alpha Antibody [NB100-449] - Analysis in HEK293 cells. Image courtesy of anonymous customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunoprecipitation-NB100-449-img0005.jpg)
![Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - Murine primary bone marrow derived macrophages stained with HIF1-alpha antibody (red). Nuclei were counterstained with Dapi (blue). Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunofluorescence-NB100-449-img0039.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in overexpression and endogenous HIF1 alpha & HIF2 alpha using anti-HIF-1 alpha antibody. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-134-img0011.jpg "<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in overexpression and endogenous HIF1 alpha & HIF2 alpha using anti-HIF-1 alpha antibody. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Image from verified customer review.")
![<b>Independent Antibodies Validation, Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of NB100-134, anti-HIF-1 alpha, on normoxic and hypoxic nuclear rat cell lysates.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-134-img0007.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: HIF-1 alpha Antibody [NB100-134] - PC-3 Cell Lysates run with Cobalt Chloride treatments. Immunoprecipitation was performed with protein A/G Agarose beads. This data was provided courtesy of Kelie Reece, Figg lab, NCI.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunoprecipitation-NB100-134-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in ARPE-19 cells using anti-HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-134-img0010.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - Staining in the canine CL on days 10 to 70 after ovulation. PC = positive control (human placenta). NC = negative control. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-134-img0008.jpg)
![Simple Western: HIF-1 alpha Antibody [NB100-134] - Image shows a specific band for HIF-1 alpha in 0.2 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Simple-Western-NB100-134-img0009.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - Analysis of FFPE tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF1 alpha antibody at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the section was further counterstained using hematoxylin. The tested section depicted mainly a diffused cytoplasmic staining but there were some cells which showed nuclear signal also (representing hypoxic cells).](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-134-img0012.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha on Zebrafish retina tissue using HIF-1 alpha antibody. Mouse tissue was used as a control. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-134-img0014.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-134] - 20 ug whole cell lysates (human ovarian cancer cell lines). Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-134-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in human Retinal and Choroidal primary endothelia cells using anti-HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-134-img0013.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody [NB100-134] - Staining of human kidney, renal tubular epithelium in Cortex.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-NB100-134-img0006.jpg)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using NB100-123.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-123-img0023.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using NB100-123.")
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-123-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining in pig endothelial cells under hypoxia condition. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunocytochemistry-Immunofluorescence-NB100-123-img0011.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis of HIF-1 alpha in human lung tissue. Image courtesy of product review by Aneta Gandjeva.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunohistochemistry-NB100-123-img0006.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining of Biotin conjugated HIF-1 alpha in human kidney.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunohistochemistry-NB100-123-img0026.jpg)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunoprecipitation of HIF-1alpha using NB100-123. Heavy and light chains are also detected. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-123-img0025.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining on pig tissue (brown). Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(H1alpha67)-Immunohistochemistry-Paraffin-NB100-123-img0012.jpg)
![Simple Western: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Image shows a specific band for HIF-1 alpha in 0.5 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Simple-Western-NB100-131-img0020.jpg "Simple Western: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Image shows a specific band for HIF-1 alpha in 0.5 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (ESEE122) [NB100-131] - HIF-1 alpha in Cos7 cells treated or untreated with CoCl2.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Western-Blot-NB100-131-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Immunocytochemistry-Immunofluorescence-NB100-131-img0017.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - HIF-1 alpha staining in hypoxia-induced human placenta.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Immunohistochemistry-NB100-131-img0025.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - nalysis of a FFPE tissue section of human renal cancer xenograft using Lot 83115 of HIF-1 alpha antibody at 1:200 dilution. The antibody generated a strong cytoplasmic staining mainly in the cancer cells. Only a fraction of cells depicted nuclear staining, while weak to negligible positivity was seen in the tumor stromal cells.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Immunohistochemistry-Paraffin-NB100-131-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with S. aureus, using NB100-131. Blue: DAPI nuclear staining. Image courtesy of Susan Alexander and Hattie Gresham, PhD.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Immunocytochemistry-Immunofluorescence-NB100-131-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line. 100X magnification. Image courtesy of Susan Alexander and Hattie Gresham, PhD.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Immunocytochemistry-Immunofluorescence-NB100-131-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with S. aureus. 100X magnification. Blue: DAPI nuclear staining.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Immunocytochemistry-Immunofluorescence-NB100-131-img0016.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Analysis of a FFPE mouse kidney tissue section using HIF-1 alpha antibody clone ESEE122 at 1ug/mL concentration. The detection was perormed using X-cell plus universal HRP polymer detection system with Vector SG chromagen substrate. Image courtesy of a product review by Steven Grover.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Immunohistochemistry-Paraffin-NB100-131-img0019.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Analysis of HIF-1 alpha in paraffin-embedded mouse kidney tissue section using anti-HIF-1 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Immunohistochemistry-Paraffin-NB100-131-img0021.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-(ESEE122)-Immunohistochemistry-Paraffin-NB100-131-img0018.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic). NB100-449](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Western Blot-NB100-900WB-img0007.jpg "Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic). NB100-449")
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-900WB] - Immunohistochemistry staining of human glioblastoma multiforme. NB100-105](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunohistochemistry-NB100-900WB-img0014.jpg)
![Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-900WB] - Flow Cytometric Analysis of HIF1-alpha in CoCl2 Treated vs. Untreated HeLa Cells. Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. One million cells were stained with 0.125ug of NB100-449 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Reduntreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Flow Cytometry-NB100-900WB-img0009.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Western Blot analysis of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26).NB100-105](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Western Blot-NB100-900WB-img0002.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Western Blot analysis of NB100-134, anti-HIF-1 alpha, on normoxic and hypoxic nuclear rat cell lysates.NB100-134](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Western Blot-NB100-900WB-img0003.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Westerm blot detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein using NB100-296. (explants were subjected to 21 and 2% oxygen for 4 hours).](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Western Blot-NB100-900WB-img0005.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-900WB] - Staining of human kidney, renal tubular epithelium in Cortex using NB100-134.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunohistochemistry-NB100-900WB-img0004.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-900WB] - Staining of placenta, villi using NB100-479.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunohistochemistry-NB100-900WB-img0013.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-900WB] - Mouse Brain, Neurons 40X. NB100-449](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunohistochemistry-NB100-900WB-img0008.jpg)
![Western Blot: HIF-1 alpha Antibody [NB100-654] - Detection of HIF-1 alpha in Cobalt Chloride treated/untreated Cos-7 nuclear extracts using NB100-654.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-654-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-654] - Detection of HIF-1 alpha in Cobalt Chloride treated/untreated Cos-7 nuclear extracts using NB100-654.")
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody [NB100-654] - Panc0813 human pancreatic cell line. Image supplied by customer using Lot C-2.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Western-Blot-NB100-654-img0004.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-654] - IHC analysis of a formalin-fixed paraffin-embedded tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF1 alpha antibody NB100-654 at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the section was further counterstained using hematoxylin. The tested section depicted mainly a diffused cytoplasmic staining but there were some cells which showed nuclear signal also (representing hypoxic cells).](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-654-img0006.jpg)
![Simple Western: HIF-1 alpha Antibody [NB100-654] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/HIF-1-alpha-Antibody-Simple-Western-NB100-654-img0005.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Western Blot-NB100-905-img0026.jpg "Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0037.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody Pack [NB100-905] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunohistochemistry-Paraffin-NB100-905-img0034.jpg)
![Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of HIF1-alpha in CoCl2 Treated vs. Untreated HeLa Cells. Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. One million cells were stained with 0.125ug of NB100-449 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Reduntreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Flow Cytometry-NB100-905-img0017.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha in Cobalt Chloride treated/untreated Cos-7 nuclear extracts using NB100-654.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Western Blot-NB100-905-img0019.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of mouse HIF1-alpha on hypoxia treated MEFs using NB 100-449.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Western Blot-NB100-905-img0020.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic).](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Western Blot-NB100-905-img0021.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure. NB100-479.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Western Blot-NB100-905-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. Blue dye is DAPI nuclear staining. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. 100X magnification. Blue dye is DAPI nuclea](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0035.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. Photos courtesy of Susan Alexander and Hattie Gresham, PhD](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0036.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining of mouse brain, neurons using NB100-449.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunohistochemistry-NB100-905-img0022.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining of placenta, villi using NB100-479.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunohistochemistry-NB100-905-img0024.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Immunohistochemical staining of human glioblastoma multiforme utilizing NB100-105.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunohistochemistry-NB100-905-img0025.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 Alpha staining in hypoxia-induced human placenta using NB 100-131.](http://images.novusbio.com/fullsize2/HIF-1 alpha Antibody Pack-Immunohistochemistry-NB100-905-img0033.jpg)
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.](http://images.novusbio.com/fullsize2/HIF-1-alpha-[Hydroxy-Pro564]-Antibody-Western-Blot-NB110-74679-img0004.jpg "Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.")