Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ch,
Dr,
Eq,
PmSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Eq, Pm
WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Review image from confirmed customer on mouse E15.5 paraffin sections.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-Paraffin-NB300-143-img0019.jpg "Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Review image from confirmed customer on mouse E15.5 paraffin sections.")
![Western Blot: GAP-43 Antibody [NB300-143] - Different tissue and cell lysates using rabbit pAb to GAP43, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] SH-SY5Y cells, [7] C6 cells. Single band at 43 kDa mark corresponds to GAP43 protein. The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43 protein](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NB300-143-img0022.jpg)
![Immunohistochemistry: GAP-43 Antibody [NB300-143] - Representative images of immunohistological staining (brown) of OMP-positive (OMP+) cells, GAP43+ immature ORNs. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Numbers of SOX2+ ORN progenitors and Ki67+ actively proliferating cells per mm of the basal layer and OMP+ mature ORNs, GAP43+ immature ORNs, and Cas3+ apoptotic cells per mm of the OE in saline or rhIGF-1-treated mice. Open circles, rectangles, and triangles represent the values for each mouse in the saline, low-IGF-1, and high-IGF-1 treated groups (each n = 6), respectively. Image collected and cropped by Cite Ab from the following publication (Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium. Front Aging Neurosci (2018)) licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-NB300-143-img0024.jpg)
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Posterior MOE sections at approximately the same region where EOG was recorded were obtained from control and Skn-1a-/- mice exposed to either water or chemicals for 2 wks. The sections were immunoreacted with antibodies against GAP43 and OMP and stained with DAPI. Confocal images were taken from olfactory turbinate II. Similar morphology and marker expression were found in four groups of mice, indicating there was no obvious tissue damage in both control and Skn-1a-/- mice after chemical exposure in the posterior MOE regions where EOG recordings were performed. Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (//eneuro.sfn.org/lookup/doi/10.1523/ENEURO.0135-17.2017), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/GAP-43 Antibody-Immunohistochemistry-Paraffin-NB300-143-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to GAP43, dilution 1:2,000 in green, and costained with mouse mAb to vimentin, dilution 1:2,000, in red. Blue: DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NB300-143-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Rat E18 mixed neuron/glia cultures with rabbit GAP43 (red) and 5B10, mouse monoclonal to MAP-tau (green).](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NB300-143-img0018.jpg)
![Western Blot: GAP-43 Antibody [NB300-143] - Western blots of homogenate of cow cerebellum stained with RPCA-GAP-43. A prominent band running at ~43kDa represents the full length GAP-43.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NB300-143-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Immunofluorescence of GAP-43 (green), a molecular marker of neurite outgrowth, demonstrates intense staining in overexpressing wild-type PS-1 (E) PC-12 cells. (Teo, et al, 2005)](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NB300-143-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Mixed neuron-glial cultures stained with RPCA-GAP43 (red), blue is DNA staining.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NB300-143-img0017.jpg)
![Immunohistochemistry: GAP-43 Antibody [NBP1-41123] - Coronal sections of the developing (A-H) eye at (A-D) E14.5, (E-H) E17.5. were immunostained for (A-H) EVA1C and (A-H) GAP43. All sections were counterstained with DAPI. EVA1C was strongly co-expressed with GAP43 by retinal cell ganglionic axons (A-H) as they exit the retina and form the optic nerve (arrowheads). Scale bars represent 100 uM. PLoS One. 2013 Sep 9;8(9):e74115. doi: 10.1371/journal.pone.0074115.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-NBP1-41123-img0009.jpg "Immunohistochemistry: GAP-43 Antibody [NBP1-41123] - Coronal sections of the developing (A-H) eye at (A-D) E14.5, (E-H) E17.5. were immunostained for (A-H) EVA1C and (A-H) GAP43. All sections were counterstained with DAPI. EVA1C was strongly co-expressed with GAP43 by retinal cell ganglionic axons (A-H) as they exit the retina and form the optic nerve (arrowheads). Scale bars represent 100 uM. PLoS One. 2013 Sep 9;8(9):e74115. doi: 10.1371/journal.pone.0074115.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP1-41123] - Staining of a mature retinal ganglion cell demonstrating axon outgrowth.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-41123-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GAP-43 Antibody [NBP1-41123] - Longitudinal [11C]GV1-57 imaging monitors mature OSN regeneration. Immunoblotting of immature neurons (GAP-43), mature neurons (OMP), and sustentacular cells (REEP6) in septal OE tissue collected from animals 2 or 11 weeks after ZnSO4 or vehicle treatment. Red arrows indicate DVR values and the corresponding immunoblots of OE tissue collected from the same individual animals 11 weeks after treatment. Purple arrows indicate DVR values representative of [11C]GV1-57 imaging 2 weeks after treatment and corresponding immunoblots of OE tissue collected from a separate cohort of animals. n = 2 per group at 2 weeks after treatment; n = 1 per group at 11 weeks after treatment. Image collected and cropped by CiteAb from the following publication (//www.jci.org/articles/view/89162), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NBP1-41123-img0008.jpg)
![Western Blot: GAP-43 Antibody [NBP1-41123] - Analysis of GAP43 in human brain protein](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NBP1-41123-img0006.jpg)
![Western Blot: GAP-43 Antibody [NBP1-41123] - Total protein from Human, Mouse and Rat brain, and SHSY-5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-GAP43 in 1% non-fat milk in TBST and detected with an anti-sheep HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NBP1-41123-img0007.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NBP2-42847-img0010.jpg "Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP2-42847] - GAP-43 antibody detects GAP-43 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAP-43 protein stained by GAP-43 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42847-img0011.jpg)
![Immunohistochemistry-Frozen: GAP-43 Antibody [NBP2-42847] - GAP-43 antibody detects GAP-43 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: GAP-43 protein stained by GAP-43 antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-Frozen-NBP2-42847-img0007.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Analysis of A. 30 ug SK-N-SH whole cell lysate/extract B. 30 ug IMR32 whole cell lysate/extract C. 30 ug SK-N-AS whole cell lysate/extract 12 % SDS-PAGE GAP43 antibody dilution: 1:10000](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NBP2-42847-img0004.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NBP2-42847-img0006.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NBP2-42847-img0008.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NBP2-42847-img0009.jpg)
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-42847] - Analysis of Cal27 xenograft. GAP43 antibody dilution: 1:500.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-Paraffin-NBP2-42847-img0002.jpg)
![Western Blot: GAP-43 Antibody [NBP1-92714] - Western blot analysis of different tissue and cell lysates using chicken pAb to GAP43, NBP1-92714, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at the 43kDa mark corresponds to GAP43. The GAP43 protein only is detected in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-NBP1-92714-img0002.jpg "Western Blot: GAP-43 Antibody [NBP1-92714] - Western blot analysis of different tissue and cell lysates using chicken pAb to GAP43, NBP1-92714, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at the 43kDa mark corresponds to GAP43. The GAP43 protein only is detected in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP1-92714] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to GAP43, NBP1-92714, dilution 1:2,000 in green, and costained with rabbit pAb to alpha-II spectrin, dilution 1:1,000 in red. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane and synapses of neuronal cells, while the spectrin antibody stains the submembraneous cytoskeleton of axons and dendrites.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunofluorescence-NBP1-92714-img0001.jpg)
![Western Blot: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of different tissue and cell lysates using mouse mAb to GAP43, NBP2-50052, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at 43 kDa mark correspond to GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-(5E8)-Western-Blot-NBP2-50052-img0005.jpg "Western Blot: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of different tissue and cell lysates using mouse mAb to GAP43, NBP2-50052, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at 43 kDa mark correspond to GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43, NBP2-50052, dilution 1:1,000, in red, and costained with chicken pAb to GFAP, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, and GFAP antibody stains astroglia.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-(5E8)-Immunocytochemistry-Immunofluorescence-NBP2-50052-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (5E8) [NBP2-50052] - Mixed rat neuronal cultures stained with NBP2-50052 (green). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-(5E8)-Immunocytochemistry-Immunofluorescence-NBP2-50052-img0003.jpg)
![Western Blot: GAP-43 Antibody [NBP3-05535] - Western blot analysis of different tissue and cell lysates using GAP-43 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at the 43kDa mark corresponds to the GAP-43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NBP3-05535-img0002.jpg "Western Blot: GAP-43 Antibody [NBP3-05535] - Western blot analysis of different tissue and cell lysates using GAP-43 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at the 43kDa mark corresponds to the GAP-43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP3-05535] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with GAP-43 Antibody at a dilution of 1:1,000, in red, and costained with chicken pAb to MAP2 at a dilution of 1:10,000, in green. The blue is DAPI staining of nuclear DNA. GAP-43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05535-img0001.jpg)
![Western Blot: GAP-43 Antibody [NB100-81934] - Analysis of extract from mouse brain tissue, using GAP43 (Ab-41) antibody (Lane 1 and 2).](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Western-Blot-NB100-81934-img0006.jpg "Western Blot: GAP-43 Antibody [NB100-81934] - Analysis of extract from mouse brain tissue, using GAP43 (Ab-41) antibody (Lane 1 and 2).")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB100-81934] - Staining of methanol-fixed Hela cells using GAP43 (Ab-41) antibody.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81934-img0004.jpg)
![Immunohistochemistry: GAP-43 Antibody [NB100-81934] - Analysis of paraffin-embedded human breast carcinoma tissue, using GAP43 (Ab-41) antibody.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-NB100-81934-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP2-54671] - Staining of human cell line SH-SY5Y shows localization to plasma membrane.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54671-img0002.jpg "Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP2-54671] - Staining of human cell line SH-SY5Y shows localization to plasma membrane.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54671] - Staining in human cerebral cortex and pancreas tissues using anti-GAP43 antibody. Corresponding GAP43 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-Paraffin-NBP2-54671-img0005.jpg)
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54671] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-Paraffin-NBP2-54671-img0003.jpg)
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54671] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-Paraffin-NBP2-54671-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP2-54668] - Staining of human cell line SH-SY5Y shows localization to plasma membrane.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54668-img0003.jpg "Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP2-54668] - Staining of human cell line SH-SY5Y shows localization to plasma membrane.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54668] - Staining in human cerebral cortex and pancreas tissues using anti-GAP43 antibody. Corresponding GAP43 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-Paraffin-NBP2-54668-img0006.jpg)
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54668] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-Paraffin-NBP2-54668-img0004.jpg)
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-54668] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize2/GAP-43-Antibody-Immunohistochemistry-Paraffin-NBP2-54668-img0005.jpg)
![Western Blot: GAP43 [p Ser41] Antibody [NB300-179] - Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize2/GAP43-[p-Ser41]-Antibody-Western-Blot-NB300-179-img0002.jpg "Western Blot: GAP43 [p Ser41] Antibody [NB300-179] - Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with lambda-phosphatase.")
![Western Blot: GAP43 Overexpression Lysate (Adult Normal) [NBP2-04214] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GAP43.](http://images.novusbio.com/fullsize/GAP43 Overexpression Lysate (Adult Normal)-Western Blot-NBP2-04214-img0001.jpg "Western Blot: GAP43 Overexpression Lysate (Adult Normal) [NBP2-04214] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GAP43.")