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AF838

4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]

(4 Publications)

100 μg

AF838

Human T cells were treated for 48 hours with 5 μg/mL PHA then stained with Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF838) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) and PE-conjugated anti-human CD3 (Catalog # FAB100P). Quad marker was set based on control antibody (Catalog # AB‑108‑C) staining.

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human 4-1BB/TNFRSF9/CD137. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for 4‑1BB/TNFRSF9/CD137 at approximately50-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

4‑1BB/TNFRSF9/CD137 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF838

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF838-SP

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AF937

4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]

(1 Review)

(2 Publications)

100 μg

AF937

Activated mouse splenocytes were stained with Rat Anti-Mouse ICOS APC‑conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Goat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Resting mouse splenocytes were stained with Rat Anti-Mouse ICOS APC‑conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Goat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with recombinant mouse 4-1BB/Fc chimera. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for 4‑1BB/TNFRSF9/CD137 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

4‑1BB/TNFRSF9/CD137 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF937) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF937

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF937-SP

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937-4B

Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF

(1 Review)

(2 Publications)

50 μg

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications: ,

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MAB9371

4-1BB/TNFRSF9/CD137 Antibody (158321)

(2 Reviews)

(2 Publications)

100 μg

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB9371-SP
Carrier Free	MAB9371-500

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DY838

Human 4-1BB/TNFRSF9 DuoSet ELISA, 15 Plate

(5 Publications)

1 Kit (for 15 Plates)

DY838

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB937

4-1BB/TNFRSF9/CD137 Antibody (158332) [Unconjugated]

500 μg

MAB937

Activated mouse splenocytes were stained with Rat Anti-Mouse ICOS APC‑conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Rat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB937) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Resting mouse splenocytes were stained with Rat Anti-Mouse ICOS APC‑conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Rat Anti-Mouse 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB937) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB937U-100UG
Alexa Fluor 405	FAB937V-100UG
Alexa Fluor 488	FAB937G-100UG
Alexa Fluor 594	FAB937T-100UG
Alexa Fluor 647	FAB937R-100UG
Alexa Fluor 700	FAB937N-100UG
Alexa Fluor 750	FAB937S-100UG
Phycoerythrin	FAB937P

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB937P
Carrier Free	MAB937-SP
Carrier Free	FAB937V-100UG
Carrier Free	FAB937U-100UG
Carrier Free	FAB937T-100UG
Carrier Free	FAB937S-100UG
Carrier Free	FAB937R-100UG
Carrier Free	FAB937N-100UG
Carrier Free	FAB937G-100UG

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MAB8381

4-1BB/TNFRSF9/CD137 Antibody (2356B) [Unconjugated]

100 μg

MAB8381

HEK293 human embryonic kidney cell line transfected with either (A) human 4-1BB/TNFRSF9/CD137 or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Human 4-1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB8381) followed by APC-conjugated Goat-anti Rabbit IgG secondary antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Western blot shows lysates of HDLM‑2 human Hodgkin's lymphoma cell line and human tonsil tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB8381) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for 4‑1BB/TNFRSF9/CD137 at approximately 32 and 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

4‑1BB/TNFRSF9/CD137 was detected in immersion fixed peripheral blood mononuclear cells (PBMCs) treated with PHA (positive control, left panel) and HEK293 human embryonic kidney cell line (negative control, right panel) using Rabbit Anti-Human 4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB8381) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB8381U-100UG
Alexa Fluor 405	FAB8381V-100UG
Alexa Fluor 488	FAB8381G-100UG
Alexa Fluor 594	FAB8381T-100UG
Alexa Fluor 647	FAB8381R-100UG
Alexa Fluor 700	FAB8381N-100UG
Alexa Fluor 750	FAB8381S-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB8381-SP
Carrier Free	FAB8381T-100UG
Carrier Free	FAB8381V-100UG
Carrier Free	FAB8381U-100UG
Carrier Free	FAB8381S-100UG
Carrier Free	FAB8381R-100UG
Carrier Free	FAB8381N-100UG
Carrier Free	FAB8381G-100UG

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NBP2-34958

Recombinant Human 4-1BB/TNFRSF9/CD137 Protein

5μg

NBP2-34958

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier-Free	NBP2-34958-20ug
Carrier-Free	NBP2-34958-1mg
Carrier-Free	NBP2-34958-100ug
Carrier-Free	NBP2-34958-250ug
Carrier-Free	NBP2-34958-500ug

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838-4B

Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF

(4 Publications)

100 μg

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

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AF9029

4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]

100 μg

AF9029

Rat splenocyteseither (A) resting or (B) activated with with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin overnight were stained with Goat Anti-Rat 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9029) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and APC-conjugated Anti-Rat CD3. Quadrant markers were set based on control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins.

4‑1BB/TNFRSF9/CD137 was detected in immersion fixed rat splenocytes treated with calcium ionomycin and PMA using Goat Anti-Rat 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9029) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reactivity:

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF9029-SP

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DY937

Mouse 4-1BB/TNFRSF9 DuoSet ELISA, 15 Plate

(2 Publications)

1 Kit (for 15 Plates)

DY937

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications:

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MAB838

4-1BB/TNFRSF9/CD137 Antibody (145501) [Unconjugated]

100 μg

MAB838

Human CD3+ peripheral blood mononuclear cells were treated for 48 hours with 5 μg/mL PHA then stained with Mouse Anti-Human4‑1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB838, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB838U-100UG
Alexa Fluor 405	FAB838V-100UG
Alexa Fluor 488	FAB838G-100UG
Alexa Fluor 594	FAB838T-100UG
Alexa Fluor 647	FAB838R-100UG
Alexa Fluor 700	FAB838N-100UG
Alexa Fluor 750	FAB838S-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB838-SP
Carrier Free	MAB838-500
Carrier Free	FAB838U-100UG
Carrier Free	FAB838T-100UG
Carrier Free	FAB838S-100UG
Carrier Free	FAB838R-100UG
Carrier Free	FAB838N-100UG
Carrier Free	FAB838G-100UG
Carrier Free	FAB838V-100UG

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

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Species Glossary

4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]

4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]

Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF

4-1BB/TNFRSF9/CD137 Antibody (158321)

Human 4-1BB/TNFRSF9 DuoSet ELISA, 15 Plate

4-1BB/TNFRSF9/CD137 Antibody (158332) [Unconjugated]

4-1BB/TNFRSF9/CD137 Antibody (2356B) [Unconjugated]

Recombinant Human 4-1BB/TNFRSF9/CD137 Protein

Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF

4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]

Mouse 4-1BB/TNFRSF9 DuoSet ELISA, 15 Plate

4-1BB/TNFRSF9/CD137 Antibody (145501) [Unconjugated]