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alpha Satellite repeat primer
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v6.5 Mouse embryonic stem cells
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1018
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1585
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1668
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Products (206)
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NBP2-29540
pCL-Eco Retrovirus Packaging Vector
(62 Publications)
10 μg
NBP2-29540
Reactivity:
Mu
,
Rt
Species Glossary
Reactivity:
Mu
,
Rt
Applications:
RetVir
Applications:
RetVir
Alt Catalog#
10045P
Alt Catalog#
10045P
compare
(select up to 3 products from this page to compare)
NBP2-29541
pCL-Ampho Retrovirus Packaging Vector
(31 Publications)
10 μg
Reactivity:
Ma
,
Ha(-)
Species Glossary
Reactivity:
Ma
,
Ha(-)
Applications:
RetVir
Applications:
RetVir
Alt Catalog#
10046P
Alt Catalog#
10046P
compare
(select up to 3 products from this page to compare)
NBP2-29542
pCL-10A1 Retrovirus Packaging Vector
(29 Publications)
10 μg
Reactivity:
Ma
Species Glossary
Reactivity:
Ma
Applications:
RetVir
Applications:
RetVir
Alt Catalog#
10047P
Alt Catalog#
10047P
compare
(select up to 3 products from this page to compare)
NBP2-25288
Polyinosinic-polycytidylic acid
(16 Publications)
10 mg
NBP2-25288
Reactivity:
Hu
,
Mu
,
Rt
Species Glossary
Reactivity:
Hu
,
Mu
,
Rt
Applications:
Func
,
In vitro
,
In vivo
,
LA
Applications:
Func
,
In vitro
,
In vivo
,
LA
Alt Catalog#
IMG-2203-10
Alt Catalog#
IMG-2203-10
compare
(select up to 3 products from this page to compare)
NBP1-41162
v6.5 Mouse embryonic stem cells
(12 Publications)
2 ml
NBP1-41162
Reactivity:
Mu
Species Glossary
Reactivity:
Mu
Applications:
In vitro
Applications:
In vitro
compare
(select up to 3 products from this page to compare)
NBP2-25297
Pam3CSK4, TLR1 and TLR2 Ligand
(19 Publications)
0.1 mg
NBP2-25297
Reactivity:
Hu
,
Mu
Species Glossary
Reactivity:
Hu
,
Mu
Applications:
Func
,
In vitro
,
In vivo
,
LA
Applications:
Func
,
In vitro
,
In vivo
,
LA
Alt Catalog#
IMG-2201-100
Alt Catalog#
IMG-2201-100
compare
(select up to 3 products from this page to compare)
NBP2-25289
Flagellin, TLR5 Ligand
(9 Publications)
0.01 mg
NBP2-25289
Reactivity:
Hu
,
Mu
Species Glossary
Reactivity:
Hu
,
Mu
Applications:
Func
,
In vitro
,
LA
Applications:
Func
,
In vitro
,
LA
Alt Catalog#
IMG-2205
Alt Catalog#
IMG-2205
compare
(select up to 3 products from this page to compare)
NBP2-26219
MALP-2, TLR6 and TLR2 ligand
(11 Publications)
2 μg
NBP2-26219
Reactivity:
Hu
,
Mu
,
Av
Species Glossary
Reactivity:
Hu
,
Mu
,
Av
Applications:
Func
,
In vitro
Applications:
Func
,
In vitro
Alt Catalog#
IMG-2206
Alt Catalog#
IMG-2206
compare
(select up to 3 products from this page to compare)
NBP2-26232
CpG oligodeoxynucleotides with negative control, TLR9 ligand
(1 Review)
(4 Publications)
2 Vials
NBP2-26232
Reactivity:
Hu
Species Glossary
Reactivity:
Hu
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IMG-2209Hpt
Alt Catalog#
IMG-2209Hpt
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NBA1-19349
DGCR8 knockout Mouse embryonic stem cells
(10 Publications)
2 ml
NBA1-19349
Reactivity:
Hu
,
Mu
Species Glossary
Reactivity:
Hu
,
Mu
Applications:
In vitro
Applications:
In vitro
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NBP2-31156
DAPI Solution
(1 Review)
1 ml
NBP2-31156
Applications:
Flow
,
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
,
ICC
,
IF
,
IHC-FrFl
,
IHC-WhMt
Applications:
Flow
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ICC/IF
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IHC
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IHC-Fr
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IHC-P
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ICC
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IF
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IHC-FrFl
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NB900-66726
Fluoromount
(1 Review)
(2 Publications)
25 ml
Applications:
ICC/IF
,
IHC
,
IHC-Fr
,
IHC-P
Applications:
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page
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18
![pCLNDX Retrovirus Expression Vector [NBP2-29540]](http://images.novusbio.com/fullsize2/pCLNDX-Retrovirus-Expression-Vector-NBP2-29540-img0001.jpg "pCLNDX Retrovirus Expression Vector [NBP2-29540]")
![Polyinosinic-polycytidylic acid [NBP2-25288] - 293T cells were co-transfected with pCMV/TLR3 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000 (Life Technology). After 48 hrs of transfection, 10 ug/ml of Poly(I:C) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit](http://images.novusbio.com/fullsize2/Polyinosinic-polycytidylic-acid-NBP2-25288-img0002.jpg "Polyinosinic-polycytidylic acid [NBP2-25288] - 293T cells were co-transfected with pCMV/TLR3 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000 (Life Technology). After 48 hrs of transfection, 10 ug/ml of Poly(I:C) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit")
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the mesoderm marker, Smooth Muscle Actin, was detected in 15 day cells using Mouse Anti-Smooth Muscle Actin monoclonal antibody (R&D Systems, Catalog # MAB1420). The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0013.jpg "Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the mesoderm marker, Smooth Muscle Actin, was detected in 15 day cells using Mouse Anti-Smooth Muscle Actin monoclonal antibody (R&D Systems, Catalog # MAB1420). The nuclei were counterstained with DAPI.")
![Flow Cytometry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Express the Pluripotency Stem Cell Marker SSEA-1 and Lack SSEA-4. v6.5 mouse embryonic stem cells (Novus Biologicals, NBP1-41162) were cultured in complete StemXVivo Mouse Pluripotent Stem Cell media. Expression of SSEA-1 and SSEA-4 were detected using PE-conjugated Mouse Anti-SSEA-1 Monoclonal Antibody (R&D Systems, Catalog # FAB2155P) and APC-conjugated Mouse Anti-SSEA-4 Monoclonal Antibody (R&D Systems, Catalog # FAB1435A. The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Flow-Cytometry-NBP1-41162-img0014.jpg)
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Express the Pluripotency Stem Cell Marker Oct-3/4. v6.5 mouse embryonic stem cells (Novus Biologicals, NBP1-41162) were cultured in complete StemXVivo Mouse Pluripotent Stem Cell media. Expression of Oct-3/4 was detected using Rat Anti-Human/Mouse Oct-3/4 Monoclonal Antibody (R&D Systems, Catalog # MAB1759) followed by NorthernLights (NL)557-conjugated Goat Anti-Rat Secondary Antibody (R&D Systems, Catalog # NL013). The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0009.jpg)
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Express the Pluripotency Stem Cell Marker Alkaline Phosphatase. v6.5 mouse embryonic stem cells (Novus Biologicals, NBP1-41162) were cultured in complete StemXVivo Mouse Pluripotent Stem Cell media. Expression of Alkaline Phosphatase was detected using Goat Anti-Mouse Alkaline Phosphatase Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF2910) followed by NL557-conjugated Donkey Anti-Goat Secondary Antibody (R&D Systems, Catalog # NL001). The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0010.jpg)
![Immunocytochemistry: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the ectoderm marker, SOX1, was detected in day 10 cells using Goat Anti-SOX1 Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF3369) followed by NorthernLights (NL)557-conjugated Donkey Anti-Goat Secondary Antibody. The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunocytochemistry-NBP1-41162-img0011.jpg)
![In vitro assay: v6.5 Mouse embryonic stem cells [NBP1-41162] - Brightfield Image of v6.5 Mouse embryonic stem cell [NBP1-41162] colonies growing on a Mitomycin C treated MEF cell feeder layer.](http://images.novusbio.com/fullsize2/v6.5 Mouse embryonic stem cells-In vitro assay-NBP1-41162-img0003.jpg)
![In vitro assay: v6.5 Mouse embryonic stem cells [NBP1-41162] - Brightfield Image of v6.5 Mouse embryonic stem cell [NBP1-41162] colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize2/v6.5 Mouse embryonic stem cells-In vitro assay-NBP1-41162-img0004.jpg)
![Immunodiffusion: v6.5 Mouse embryonic stem cells [NBP1-41162] - Embryonic Stem Cells Grown in Mouse Pluripotent Stem Cell Media Are Capable of Tri-Lineage Differentiation. v6.5 mouse embryonic stem cells were cultured in complete StemXVivo Mouse Pluripotent Stem Cell Media. For differentiation, cells were cultured as embryoid bodies for 5 days, plated onto Cultrex BME-coated plates (R&D Systems, Catalog # 3434-005-02) for an additional 5-10 days. Expression of the endoderm marker, SOX17, was detected in 10 day cells using Goat Anti-SOX17 Affinity Purified Polyclonal Antibody (R&D Systems, Catalog # AF1924) followed by NL557-conjugated Donkey Anti-Goat Secondary Antibody. The nuclei were counterstained with DAPI.](http://images.novusbio.com/fullsize2/v6.5-Mouse-embryonic-stem-cells-Immunodiffusion-NBP1-41162-img0012.jpg)
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.](http://images.novusbio.com/fullsize2/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0004.jpg "Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.")
![Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Evaluation of the ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line. IML-102 cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase reporter gene under the transcriptional control of an NF-KB response element. IML-102 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: specifically activated the TLR2/1-depedent NF-KB/SEAP reporter cells in a dose dependent manner (Note: HEK 293 cells endogenously express TLR1).](http://images.novusbio.com/fullsize2/Pam3CSK4--TLR1-and-TLR2-Ligand-NBP2-25297-img0005.jpg)
![Flagellin, TLR5 Ligand [NBP2-25289] - TLR5 [NBP2-25289] - Flagellin specifically activated the -depedent NF-KB/SEAP reporter cells in a dose dependent manner but did not activate TLR2 or TLR4.](http://images.novusbio.com/fullsize2/Flagellin--TLR5-Ligand-NBP2-25289-img0003.jpg "Flagellin, TLR5 Ligand [NBP2-25289] - TLR5 [NBP2-25289] - Flagellin specifically activated the -depedent NF-KB/SEAP reporter cells in a dose dependent manner but did not activate TLR2 or TLR4.")
![Flagellin, TLR5 Ligand [NBP2-25289] - TLR5 [NBP2-25289] - Flagellin specifically activated the TLR5-depedent NF-KB/SEAP reporter cells in a dose dependent manner but did not activate TLR2 or TLR4.](http://images.novusbio.com/fullsize2/Flagellin--TLR5-Ligand-NBP2-25289-img0004.jpg)
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/ml of MALP2 as added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit.](http://images.novusbio.com/fullsize2/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0003.jpg "MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/ml of MALP2 as added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit.")
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Evaluation of the MALP2 ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line.Cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. Cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of MALP2 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: MALP2 specifically activated the TLR2/6-depedent NF-kB/SEAP reporter cells in a dose dependent manner (Note: HEK 293 cells endogenously express TLR6).](http://images.novusbio.com/fullsize2/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0004.jpg)
![CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and TLR9/HEK 293 cell lines were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.](http://images.novusbio.com/fullsize2/CpG-oligodeoxynucleotides-with-negative-control--TLR9-ligand-NBP2-26232-img0002.jpg "CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and TLR9/HEK 293 cell lines were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.")
![Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBA1-19349-img0008.jpg "Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Flow Cytometry: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - DCGR8 mouse embryonic stem cells were cultured for 5 passages in StemXVivo Mouse Pluripotent Stem Cell Media (R&D Systems, Catalog # CCM025). At passage 5, cells were harvested and analyzed for pluripotent markers by flow cytometry. Expression of SSEA-1 and SSEA-4 were detected using PE-conjugated Mouse Anti-SSEA-1 Monoclonal Antibody (R&D Systems, Catalog # FAB2155P) and APC-conjugated Mouse Anti-SSEA-4 Monoclonal Antibody (R&D Systems, Catalog # FAB1435A). Quadrant lines were drawn based on matched isotype controls.](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-Flow-Cytometry-NBA1-19349-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Nanog antibody (NB100-58842) was tested in with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBA1-19349-img0007.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0009.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing on a Mitomycin C MEF cell feeder layer.](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0010.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 -/- ES cell colonies growing on a gamma-irradiated MEF cell feeder layer.](http://images.novusbio.com/fullsize2/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - ICC analysis of LM7 cells using anti-IgM antibody [DyLight 550] (Cat.# NBP2-34422R). (blue) was used as a nuclear counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize2/DAPI-Solution-Immunocytochemistry-Immunofluorescence-NBP2-31156-img0001.jpg "Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - ICC analysis of LM7 cells using anti-IgM antibody [DyLight 550] (Cat.# NBP2-34422R). (blue) was used as a nuclear counterstain. Image from verified customer review.")
![Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha tubulin in Hela cell line. Cells, cultured on cover slips, were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 1:200 dilution of anti-58K Golgi Protein antibody clone 58K-9 for overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) and the cells were then imaged using a 40X objective.](http://images.novusbio.com/fullsize2/DAPI-Solution-Immunofluorescence-NBP2-31156-img0002.jpg)