Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.
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![Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.](http://images.novusbio.com/fullsize2/Human-Brain-Whole-Tissue-Lysate-(Adult-Normal)-Western-Blot-NB820-59177-img0001.jpg "Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.")
![Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize2/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0005.jpg "Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.")
![Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Western Blot: [NBP2-49688] - 10 ug of Chloroquine treated (+) and untreated (-) Neuro2A lysates in 1x Laemmli sample buffer (NBP2-49688) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0004.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize2/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0005.jpg "Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.")
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - 10 ug of Chloroquine treated (+) and untreated (-) HeLa lysates in 1x Laemmli sample buffer (NBP2-49689) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0002.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - Human Cervical carcinoma cells (HeLa) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49689) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize2/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0003.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with vehicle (-) or with 50 uM chloriquine (+) for 24 hours and the total cell lysates were prepared. The lysates were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF mebbrane and blocked in 5% non-fat milk. The membrane was then probed with 1 ug/ml of p62/SQSTM1 antibody (NBP1-48320) in 1% milk and dectected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize2/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0004.jpg)
![Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].](http://images.novusbio.com/fullsize2/HepG2 Whole Cell Lysate-Western Blot-NBP1-42569-img0004.jpg "Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].")
![SDS-Page: HepG2 Whole Cell Lysate [NBP1-42569] - Ponceau S staining of HepG2 whole cell lysate using NBP1-42569.](http://images.novusbio.com/fullsize2/HepG2 Whole Cell Lysate-SDS-Page-NBP1-42569-img0001.jpg)
![Western Blot: HeLa Hypoxic / Normoxic Tissue Lysate [NBP2-36452] - Western blot analysis of HIF-1 alpha antibody (NB100-134) in HeLa hypoxic and normoxic lysates (NBP2-36452). Tubulin loading control is shown below.](http://images.novusbio.com/fullsize2/HeLa-Hypoxic---Normoxic-Tissue-Lysate-Western-Blot-NBP2-36452-img0002.jpg "Western Blot: HeLa Hypoxic / Normoxic Tissue Lysate [NBP2-36452] - Western blot analysis of HIF-1 alpha antibody (NB100-134) in HeLa hypoxic and normoxic lysates (NBP2-36452). Tubulin loading control is shown below.")
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.](http://images.novusbio.com/fullsize2/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0007.jpg "Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.")
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Ponceau S staining of HeLa whole cell lysate using NB800-PC1.](http://images.novusbio.com/fullsize2/HeLa Whole Cell Lysate-Western Blot-NB800-PC1-img0003.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Analysis of STIM-1 antibody (NB110-60547) using HeLa whole cell lysate [NB800-PC1].](http://images.novusbio.com/fullsize2/HeLa Whole Cell Lysate-Western Blot-NB800-PC1-img0004.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of 30ug of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal Survivin antibody (NB 500-201) at 1ug/ml concentration. Detection was performed using ECL based method with 1 minute exposure time.](http://images.novusbio.com/fullsize2/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0005.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal NBS1 antibody (NB100-143) with detection employing ECL method.](http://images.novusbio.com/fullsize2/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0006.jpg)
![Western Blot: Human Testis Whole Tissue Lysate (Adult Normal) [NB820-59266] - Detection of NUT and GAPDH in Human Testis Whole Tissue Lysate (cat# NB820-59266) and rat testis lysate. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize2/Human-Testis-Whole-Tissue-Lysate-(Adult-Normal)-Western-Blot-NB820-59266-img0001.jpg "Western Blot: Human Testis Whole Tissue Lysate (Adult Normal) [NB820-59266] - Detection of NUT and GAPDH in Human Testis Whole Tissue Lysate (cat# NB820-59266) and rat testis lysate. Image provided via product review by verified customer.")
![Western Blot: Human Lung Whole Tissue Lysate (Adult Normal) [NB820-59239] - analysis of Beta Actin in human lung tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.](http://images.novusbio.com/fullsize2/Human-Lung-Whole-Tissue-Lysate-(Adult-Normal)-Western-Blot-NB820-59239-img0001.jpg "Western Blot: Human Lung Whole Tissue Lysate (Adult Normal) [NB820-59239] - analysis of Beta Actin in human lung tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.")
![Western Blot: Human Colon Whole Tissue Lysate (Adult Normal) [NB820-59205] - analysis of Beta Actin in human colon tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.](http://images.novusbio.com/fullsize2/Human-Colon-Whole-Tissue-Lysate-(Adult-Normal)-Western-Blot-NB820-59205-img0001.jpg "Western Blot: Human Colon Whole Tissue Lysate (Adult Normal) [NB820-59205] - analysis of Beta Actin in human colon tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.")
![SDS-Page: Human Colon Whole Tissue Lysate (Whole Normal) [NB820-59205]](http://images.novusbio.com/fullsize2/Human-Colon-Whole-Tissue-Lysate-(Whole-Normal)-SDS-Page-NB820-59205-img0002.jpg)
![Western Blot: Human Pancreas Whole Tissue Lysate (Adult Whole Normal) [NB820-59244] - man Pancreas Whole Tissue Lysate (Adult Normal) [NB820-59244] - Image from verified customer review.](http://images.novusbio.com/fullsize2/Human-Pancreas-Whole-Tissue-Lysate-(Adult-Whole-Normal)-Western-Blot-NB820-59244-img0002.jpg "Western Blot: Human Pancreas Whole Tissue Lysate (Adult Whole Normal) [NB820-59244] - man Pancreas Whole Tissue Lysate (Adult Normal) [NB820-59244] - Image from verified customer review.")
![Western Blot: Human Placenta Whole Tissue Lysate (Adult Whole Normal) [NB820-59248] - Used as a positive control for human PiT-1 (SLC20A1) protein on an anti-human PiT-1 Western blot using human plancenta lysate. Primary antibody @ 1:1000, secondary antibody @ 1:5000, visualization by ECL. Image from verified customer review.](http://images.novusbio.com/fullsize2/Human-Placenta-Whole-Tissue-Lysate-(Adult-Whole-Normal)-Western-Blot-NB820-59248-img0001.jpg "Western Blot: Human Placenta Whole Tissue Lysate (Adult Whole Normal) [NB820-59248] - Used as a positive control for human PiT-1 (SLC20A1) protein on an anti-human PiT-1 Western blot using human plancenta lysate. Primary antibody @ 1:1000, secondary antibody @ 1:5000, visualization by ECL. Image from verified customer review.")