Product Search for Immunohistochemistry Paraffin: Novus Biologicals

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Antibody Validation

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1085 Results for "Immunohistochemistry Paraffin"

NB100-105

HIF-1 alpha Antibody (H1alpha67)

(32 Reviews)

(714 Publications)

0.1 ml

NB100-105

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.

<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout Hela cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.

<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.

<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis of HIF-1 alpha in human hepatocytes from cancer patient using anti-HIF-1 alpha antibody. Image from verified customer review.

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Biotin conjugate of NB100-105. Staining of human glioblastoma multiforme.

Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Expression of HIF-1 in intresititial pig cells under 12% of 02 condition. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1a was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFOA using 1 ug/mL of mouse anti-HIF-1a monoclonal (NB100-105, Novus Biologicals). Cells were stained using donkey anti-rabbit and counterstained with DAPI (blue).

Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in H929 cells using HIF-1 alpha antibody. Image from verified customer review.

Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1a antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine.

Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Figure Legend: Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1a-deficient (Hif1a KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1a and lamin B1 levels were analyzed by Western blot. This image was submitted via customer Review. (Mouse, WB)

Reactivity:

Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp

Applications:

, , , , , , , , , , , , ,

Applications: , , , , , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NB100-105AF405
Alexa Fluor 488	NB100-105AF488
Alexa Fluor 647	NB100-105AF647
Alexa Fluor 700	NB100-105AF700
Allophycocyanin	NB100-105APC
Biotin	NB100-105B
DyLight 350	NB100-105UV
DyLight 405	NB100-105V
DyLight 488	NB100-105G
DyLight 550	NB100-105R
DyLight 650	NB100-105C
DyLight 680	NB100-105FR
DyLight 755	NB100-105IR
FITC	NB100-105F
HRP	NB100-105H
PE	NB100-105PE
PerCP	NB100-105PCP

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NB100-122

HIF-2 alpha/EPAS1 Antibody

(27 Reviews)

(524 Publications)

0.1 ml

NB100-122

Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Western blot of 786-O cells without or with VHL overexpression. Image from verified customer review.

<b>Independent Antibodies Validation, Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis of HIF-2 alpha in MDA-MB-231 cell lysate (overexpression and endogenous samples) using anti-HIF-2 alpha antibody. The data showed that HIF-2 alpha antibody did not react to HIF-1 alpha overexpression. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF-2 alpha antibody was tested in RCC4 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.

<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis of HIF-2 alpha in human Retinal and Choroidal primary endothelia lysates using anti-HIF-2 alpha antibody. Image from verified customer review.

<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis using the HRP conjugate of NB100-122. Detection of normoxic and hypoxic nuclear rat cell lysates.

<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using NB100-122.

Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF2 expression in HIF2 over-expressed HEK 293T cells. Image from verified customer review.

Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Hif-2 alpha immunoreactivity in human cardiac myocytes stained with NB100-122.

<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis on normoxic and hypoxic nuclear rat cell lysates.

Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Western Blot on Human Glioma cells, whole cell lysates from a customer review.

Simple Western: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Simple Western lane view shows a specific band for HIF-2 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.