Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Dr,
GP,
Pm,
Rb,
Xp,
YeSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Dr, GP, Pm, Rb, Xp, Ye
WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-1-Antibody-(464.6)-Western-Blot-NB300-146-img0013.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.")
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Immunocytochemistry: Detection of ATPA1 (Green) in HepG2 cells using NB300-146. Nuclei (Blue) were counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-1-Antibody-(464.6)-Immunocytochemistry-Immunofluorescence-NB300-146-img0007.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Staining of human enodmetrial glands within the uterus using NB300-146. Note the absence of staining in the surrounding myometrial smooth muscle.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-1-Antibody-(464.6)-Immunohistochemistry-NB300-146-img0012.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-1-Antibody-(464.6)-Flow-Cytometry-NB300-146-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-1-Antibody-(464.6)-Immunocytochemistry-Immunofluorescence-NB300-146-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-1-Antibody-(464.6)-Immunocytochemistry-Immunofluorescence-NB300-146-img0015.jpg)
![Western Blot: Glut1 Antibody [NB110-39113] - Analysis of GLUT1 on A. mouse kidney membrane protein and B. rat kidney membrane protein.](http://images.novusbio.com/fullsize2/Glut1-Antibody-Western-Blot-NB110-39113-img0006.jpg "Western Blot: Glut1 Antibody [NB110-39113] - Analysis of GLUT1 on A. mouse kidney membrane protein and B. rat kidney membrane protein.")
![Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize2/Glut1-Antibody-Western-Blot-NB110-39113-img0007.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.](http://images.novusbio.com/fullsize2/Glut1-Antibody-Immunohistochemistry-Paraffin-NB110-39113-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: Glut1 Antibody [NB110-39113] - Analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. FMO control (red) and isotype control (blue, NBP2-24983) were compared to CD4+ T cells (orange), and this PE conjugated Glut 1 antibody positively stained CD4+ lymphocytes isolated from Mouse (Image submitted by Verified Customer).](http://images.novusbio.com/fullsize2/Glut1-Antibody-Flow-Cytometry-NB110-39113-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NB110-39113] - GLUT1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/Glut1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-39113-img0003.jpg)
![Western Blot: Glut1 Antibody [NB110-39113] - Glut 1 at 55kDa. Antibody diluted 1:500. This image was submitted via customer Review. *{Human WB}*](http://images.novusbio.com/fullsize2/Glut1-Antibody-Western-Blot-NB110-39113-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NB110-39113] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective](http://images.novusbio.com/fullsize2/Glut1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-39113-img0013.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.](http://images.novusbio.com/fullsize2/Glut1-Antibody-Immunohistochemistry-Paraffin-NB110-39113-img0012.jpg)
![Flow (Intracellular): Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG2 with Glut1 Antibody NB110-39113 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize2/Glut1-Antibody-Flow-(Intracellular)-NB110-39113-img0017.jpg)
![Western Blot: Glut1 Antibody [NB110-39113] - GLUT 1 in A549 cells. Image from verified customer review.](http://images.novusbio.com/fullsize2/Glut1-Antibody-Western-Blot-NB110-39113-img0008.jpg)
![Western Blot: Glut1 Antibody [NB300-666] - WB analysis of purified Glucose Transporter (GLUT1) with GLUT1 antibody at a dilution of 1:2000.](http://images.novusbio.com/fullsize2/Glut1-Antibody-Western-Blot-NB300-666-img0001.jpg "Western Blot: Glut1 Antibody [NB300-666] - WB analysis of purified Glucose Transporter (GLUT1) with GLUT1 antibody at a dilution of 1:2000.")
![Western Blot: Glut1 Antibody [NB300-666] - Rat brain cortical extracts (10ug total protein/lane) on 10% SDS-PAGE gel. Diluted 1:2000.](http://images.novusbio.com/fullsize2/Glut1-Antibody-Western-Blot-NB300-666-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-3-Antibody-(XVIF9-G10)-Immunocytochemistry-Immunofluorescence-NB300-540-img0009.jpg "Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-3-Antibody-(XVIF9-G10)-Immunohistochemistry-Paraffin-NB300-540-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-3-Antibody-(XVIF9-G10)-Immunocytochemistry-Immunofluorescence-NB300-540-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in HeLa cells is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-3-Antibody-(XVIF9-G10)-Immunocytochemistry-Immunofluorescence-NB300-540-img0007.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-3-Antibody-(XVIF9-G10)-Immunohistochemistry-Paraffin-NB300-540-img0011.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human colon tissues.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-3-Antibody-(XVIF9-G10)-Immunohistochemistry-Paraffin-NB300-540-img0012.jpg)
![Simple Western: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Simple Western lane view shows a specific band for Sodium Potassium ATPase Alpha 3 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Alpha-3-Antibody-(XVIF9-G10)-Simple-Western-NB300-540-img0010.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.](http://images.novusbio.com/fullsize2/Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A))-Western Blot-NB300-147-img0005.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.")
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Sodium Potassium ATPase Beta 1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/Sodium-Potassium-ATPase-Beta-1-Antibody-(464.8-(also-known-as-8A))-Immunocytochemistry-Immunofluorescence-NB300-147-img0008.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using NB 300-147. Lane 1: 1:2,500 primary. Lane 2: 1:5,000 primary.](http://images.novusbio.com/fullsize2/Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A))-Western Blot-NB300-147-img0007.jpg)
![Western Blot: Syndecan-4 Antibody [NB110-41551] - Analysis of Syndecan-4 with Jurkat cell lysate.](http://images.novusbio.com/fullsize2/Syndecan-4-Antibody-Western-Blot-NB110-41551-img0004.jpg "Western Blot: Syndecan-4 Antibody [NB110-41551] - Analysis of Syndecan-4 with Jurkat cell lysate.")
![Immunohistochemistry-Paraffin: Syndecan-4 Antibody [NB110-41551] - Formalin fixed, paraffin-embedded tissue after heat-induced antigen retrieval. 5 ug/ml of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize2/Syndecan-4-Antibody-Immunohistochemistry-Paraffin-NB110-41551-img0003.jpg)
![Western Blot: pan Cadherin Antibody (CH-19) [NB100-1907] - Analysis of pan Cadherin Antibody (CH-19) in Madin Darby Bovine Kidney (MDBK) extract.](http://images.novusbio.com/fullsize2/pan-Cadherin-Antibody-(CH-19)-Western-Blot-NB100-1907-img0001.jpg "Western Blot: pan Cadherin Antibody (CH-19) [NB100-1907] - Analysis of pan Cadherin Antibody (CH-19) in Madin Darby Bovine Kidney (MDBK) extract.")
![Immunohistochemistry-Paraffin: pan Cadherin Antibody (CH-19) [NB100-1907] - Analysis of pan Cadherin formalin-fixed paraffin embedded human breast carcinoma.](http://images.novusbio.com/fullsize2/pan-Cadherin-Antibody-(CH-19)-Immunohistochemistry-Paraffin-NB100-1907-img0002.jpg)
![Western Blot: pan Cadherin Antibody (PC-79) [NBP1-41361] - HEK 293T whole cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize2/pan-Cadherin-Antibody-(PC-79)-Western-Blot-NBP1-41361-img0001.jpg "Western Blot: pan Cadherin Antibody (PC-79) [NBP1-41361] - HEK 293T whole cell lysate. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Syndecan-4 Antibody [NBP1-90173] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.](http://images.novusbio.com/fullsize2/Syndecan-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90173-img0004.jpg "Immunocytochemistry/Immunofluorescence: Syndecan-4 Antibody [NBP1-90173] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.")
![Immunohistochemistry-Paraffin: Syndecan-4 Antibody [NBP1-90173] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/Syndecan-4 Antibody-Immunohistochemistry-Paraffin-NBP1-90173-img0005.jpg)
![Immunohistochemistry-Paraffin: GLUT1 Antibody [NB120-15309] - Staining of human bladder](http://images.novusbio.com/fullsize2/GLUT1 Antibody-Immunohistochemistry-Paraffin-NB120-15309-img0002.jpg "Immunohistochemistry-Paraffin: GLUT1 Antibody [NB120-15309] - Staining of human bladder")
![Immunohistochemistry-Paraffin: GLUT1 Antibody [NB120-15309] - Formalin fixed paraffin embedded human esophagus stained with GLUT-1 antibody.](http://images.novusbio.com/fullsize2/GLUT1 Antibody-Immunohistochemistry-Paraffin-NB120-15309-img0001.jpg)
![Immunohistochemistry-Frozen: Integrin alpha 2/CD49b Antibody (MM0415-2B13) [NBP2-11736] - 10% Buffer formalin fixed and parffine embedded human placental tissue section (4um) is subjected IHC staining using NBP2-11736. Tissue section was pretreated in citric buffer (ph6.0) with microwave for antige retrieval before IHC is applied.](http://images.novusbio.com/fullsize2/Integrin-alpha-2-CD49b-Antibody-(MM0415-2B13)-Immunohistochemistry-Frozen-NBP2-11736-img0001.jpg "Immunohistochemistry-Frozen: Integrin alpha 2/CD49b Antibody (MM0415-2B13) [NBP2-11736] - 10% Buffer formalin fixed and parffine embedded human placental tissue section (4um) is subjected IHC staining using NBP2-11736. Tissue section was pretreated in citric buffer (ph6.0) with microwave for antige retrieval before IHC is applied.")
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody [NB110-55540] - Analysis of anti-ITGA2 / CD49b antibody with human colon, surface epithelium at concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/Integrin-alpha-2-CD49b-Antibody-Immunohistochemistry-Paraffin-NB110-55540-img0001.jpg "Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody [NB110-55540] - Analysis of anti-ITGA2 / CD49b antibody with human colon, surface epithelium at concentration 10 ug/ml.")