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![Western Blot: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Un-treated (-) and treated (+, 0.4uM Trichostatin A treatment for 18hr) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys16) antibody.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Western-Blot-NBP2-42858-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Un-treated (-) and treated (+, 0.4uM Trichostatin A treatment for 18hr) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys16) antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys16) protein stained by Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunocytochemistry-Immunofluorescence-NBP2-42858-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Rat kidney. Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [1271] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of mouse brain. Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of mouse kidney. Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0002.jpg)
![Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Rat brain. Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [1271] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-NBP2-42858-img0006.jpg)
![Histone H4K16ac (Acetyl Lys16) antibody [GT1271] detects Histone H4K16ac (Acetyl Lys16) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [GT1271] (NBP2-42858) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-42858_mouse-monoclonal-histone-h4-ac-lys16-antibody-1271-immunohistochemistry-paraffin-261120251828104.jpg)
![Histone H4K16ac (Acetyl Lys16) antibody [GT1271] detects Histone H4K16ac (Acetyl Lys16) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [GT1271] (NBP2-42858) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-42858_mouse-monoclonal-histone-h4-ac-lys16-antibody-1271-immunohistochemistry-paraffin-261120251835434.jpg)
![Histone H4 (acetyl Lys16) antibody [GT1271] detects Histone H4 (acetyl Lys16) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (acetyl Lys16) protein stained by Histone H4 (acetyl Lys16) antibody [GT1271] (NBP2-42858) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-42858_mouse-monoclonal-histone-h4-ac-lys16-antibody-1271-immunocytochemistry-immunofluorescence-261120251848237.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, using RM204 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26012_rabbit-histone-h4-mab-rm204-azide-and-bsa-free-2462024132637.png "Western Blot: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, using RM204 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - RM204 specifically reacts to Histone H4 acetylated at Lysine 16 (K16ac). No cross reactivity with unmodified Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/nbp3-26012_rabbit-histone-h4-mab-rm204-azide-and-bsa-free-22620242311017.png)
![Western Blot: Histone H4 [ac Lys16] Antibody (RM204) [NBP2-61551] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61551, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-RM204-Western-Blot-NBP2-61551-img0004.jpg "Western Blot: Histone H4 [ac Lys16] Antibody (RM204) [NBP2-61551] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61551, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.")