| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3074-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3074-SP | |||
| Carrier Free | MAB3074-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB78591-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7859-SP | |||
![N/A Thrombospondin-1 [HRP]](http://images.novusbio.com/fullsize2/Thrombospondin-1_DTSP10_ELISA_199.jpg "N/A Thrombospondin-1 [HRP]")
![Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Representative photomicrographs showing Thrombospondin-1 (A) immunostaining in duodenal sections of 1 non-CD controls (NC) and 1 active celiac disease (ACD) patient. Isotype control staining is also shown. Right panels: positive cells were counted in at least 5 fields/section of duodenal samples of 5 NC and 5 ACD patients and expressed as mean +/- SD of all experiments. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0100980), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-NB100-2059-img0015.jpg "Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Representative photomicrographs showing Thrombospondin-1 (A) immunostaining in duodenal sections of 1 non-CD controls (NC) and 1 active celiac disease (ACD) patient. Isotype control staining is also shown. Right panels: positive cells were counted in at least 5 fields/section of duodenal samples of 5 NC and 5 ACD patients and expressed as mean +/- SD of all experiments. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0100980), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Staining in human placenta.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-Paraffin-NB100-2059-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Ang II increases the number of TSP-1 positive cells in mouse heart and skin.IHC staining of TSP-1 was performed on paraffin sections from the skin of PBS and Ang II treated WT mice. Representative photographs are shown from five animals per group. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0109763), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-Paraffin-NB100-2059-img0013.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Ang II increases the number of TSP-1 positive cells in mouse heart and skin.IHC staining of TSP-1 was performed on paraffin sections from the heart of PBS and Ang II treated WT mice. Representative photographs are shown from five animals per group. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0109763), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-Paraffin-NB100-2059-img0014.jpg)
![Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Reduced levels of vascular & pericyte markers in MMP12KO mice.A. IHC staining of vWF, TSP-1 & PDGFR beta was performed on paraffin sections from the heart of PBS & Ang II treated WT & MMP12KO mice. B. IHC staining of vWF, & PDGFR beta was performed on paraffin sections from the skin of PBS & Ang II treated WT & MMP12KO mice. Representative photographs are shown from three animals per group. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0109763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2059_mouse-monoclonal-thrombospondin-1-antibody-a6-1-310202415304261.jpg)
![N/A Thrombospondin-1 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Thrombospondin1_DY3074_ELISA_1913.jpg "N/A Thrombospondin-1 [Biotin]")
![Western Blot: Thrombospondin-1 Antibody [NBP1-52410] - (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Western-Blot-NBP1-52410-img0010.jpg "Western Blot: Thrombospondin-1 Antibody [NBP1-52410] - (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane staining.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-52410-img0008.jpg)
![Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of anti-THBS1 / Thrombospondin antibody with human placenta at concentration 3.75 ug/ml.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-52410-img0006.jpg)
![Flow Cytometry: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Flow-Cytometry-NBP1-52410-img0012.jpg)
![Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of anti-THBS1 / Thrombospondin antibody with human spleen at concentration 3.75 ug/ml.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-52410-img0004.jpg)
![Flow Cytometry: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of anti-THBS1 / Thrombospondin antibody with Spleenocytes. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Flow-Cytometry-NBP1-52410-img0003.jpg)
![Western Blot: Thrombospondin-1 Antibody [NBP2-42522] - (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Western-Blot-NBP2-42522-img0007.jpg "Western Blot: Thrombospondin-1 Antibody [NBP2-42522] - (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence")
![Immunocytochemistry/Immunofluorescence: Thrombospondin-1 Antibody [NBP2-42522] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42522-img0010.jpg)
![Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP2-42522] - (5ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-42522-img0012.jpg)
![Flow Cytometry: Thrombospondin-1 Antibody [NBP2-42522] - analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Flow-Cytometry-NBP2-42522-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Thrombospondin-1 Antibody [NBP2-42522] - analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42522-img0009.jpg)
![Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP2-42522] - staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-42522-img0005.jpg)
![Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP2-42522] - staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-42522-img0006.jpg)
![Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP2-42522] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-42522-img0011.jpg)
![Western Blot: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Western blot analysis of Thrombospondin-1 on different lysates with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/2,000 dilution. Lane 1: HUVEC cell lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat spleen tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 129 kDa Observed band size: 140kDa,170kDa,180 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33012) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33012_rabbit-thrombospondin-1-mab-psh02-97-288202412493460.jpg "Western Blot: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Western blot analysis of Thrombospondin-1 on different lysates with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/2,000 dilution. Lane 1: HUVEC cell lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat spleen tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 129 kDa Observed band size: 140kDa,170kDa,180 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33012) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling Thrombospondin-1 with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33012, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33012_rabbit-thrombospondin-1-mab-psh02-97-298202414304130.jpg)
![Immunohistochemistry: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33012) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33012_rabbit-thrombospondin-1-mab-psh02-97-298202413585520.jpg)
![Immunocytochemistry/ Immunofluorescence: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Immunocytochemistry analysis of NIH/3T3 cells labeling Thrombospondin-1 with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33012_rabbit-thrombospondin-1-mab-psh02-97-29202410484881.jpg)
![Flow Cytometry: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Flow cytometric analysis of NIH/3T3 cells labeling Thrombospondin-1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33012, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33012_rabbit-thrombospondin-1-mab-psh02-97-29202416372162.jpg)