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AF5415

Progesterone R/NR3C3 Antibody [Unconjugated]

(2 Publications)

100 μg

AF5415

Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PR-A and PR-B at approximately 90 and 118 kDa, respectively (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Progesterone R/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Progesterone R/NR3C3 at approximately 122 kDa (as indicated) using 25 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF5415U-100UG
Alexa Fluor 405	AF5415V-100UG
Alexa Fluor 488	AF5415G-100UG
Alexa Fluor 532	AF5415X-100UG
Alexa Fluor 594	AF5415T-100UG
Alexa Fluor 647	AF5415R-100UG
Alexa Fluor 700	AF5415N-100UG
Alexa Fluor 750	AF5415S-100UG
Allophycocyanin	AF5415A
Biotin	AF5415B
CoraFluor 1	AF5415CL1
DyLight 350	AF5415D
DyLight 405	AF5415E
DyLight 488	AF5415K
DyLight 550	AF5415L
DyLight 594	AF5415M
DyLight 650	AF5415W
DyLight 680	AF5415Y
DyLight 755	AF5415Z
FITC	AF5415F
HRP	AF5415H
Janelia Fluor 525	AF5415JF525
Janelia Fluor 549	AF5415I
Janelia Fluor 646	AF5415J
Janelia Fluor 669	AF5415JF669
PE	AF5415P
PerCP	AF5415C
mFluor Violet 450 SE	AF5415MFV450
mFluor Violet 500 SE	AF5415MFV500
mFluor Violet 610 SE	AF5415MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5415-SP

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NBP2-33324

Progesterone R/NR3C3 Antibody (PR484)

(1 Publication)

0.1 mg

NBP2-33324

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with Progesterone R/NR3C3 Mouse Monoclonal Antibody (PR484).

Simple Western: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324] - Simple Western lane view shows a specific band for Progesterone R/NR3C3 in 0.2 mg/ml of T47d (left) and MCF-7 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Progesterone R/NR3C3 Antibody (PR484) [NBP2-33324] - Electropherogram image of the corresponding Simple Western lane. Progesterone R/NR3C3 antibody was used at 10 ug/ml dilution of T47d and MCF-7 lysates(s) respectively.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33325AF350
Alexa Fluor 405	NBP2-33325AF405
Alexa Fluor 488	NBP2-33325AF488
Alexa Fluor 532	NBP2-33325AF532
Alexa Fluor 594	NBP2-33325AF594
Alexa Fluor 647	NBP2-33325AF647
Alexa Fluor 700	NBP2-33325AF700
Alexa Fluor 750	NBP2-33325AF750
Allophycocyanin	NBP2-33325APC
Biotin	NBP2-33325B
CoraFluor 1	NBP2-33325CL1
DyLight 350	NBP2-33325UV
DyLight 405	NBP2-33325V
DyLight 488	NBP2-33325G
DyLight 550	NBP2-33325R
DyLight 594	NBP2-33325DL594
DyLight 650	NBP2-33325C
DyLight 680	NBP2-33325FR
DyLight 755	NBP2-33325IR
FITC	NBP2-33325F
HRP	NBP2-33325H
Janelia Fluor 525	NBP2-33325JF525
Janelia Fluor 549	NBP2-33325JF549
Janelia Fluor 646	NBP2-33325JF646
Janelia Fluor 669	NBP2-33325JF669
PE	NBP2-33325PE
PerCP	NBP2-33325PCP
mFluor Violet 450 SE	NBP2-33325MFV450
mFluor Violet 500 SE	NBP2-33325MFV500
mFluor Violet 610 SE	NBP2-33325MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33325-0.1mg
IHC-Prediluted	NBP2-44916

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NBP2-33325

Progesterone R/NR3C3 Antibody (PR484) - Azide and BSA Free

0.1 mg

NBP2-33325

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR484) - Azide and BSA Free [NBP2-33325] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with Progesterone R/NR3C3 Mouse Monoclonal Antibody (PR484).

Simple Western: Progesterone R/NR3C3 Antibody (PR484) - Azide and BSA Free [NBP2-33325] - Simple Western lane view shows a specific band for Progesterone R/NR3C3 in 0.2 mg/ml of T47d (left) and MCF-7 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Progesterone R/NR3C3 Antibody (PR484) - Azide and BSA Free [NBP2-33325] - Electropherogram image of the corresponding Simple Western lane. Progesterone R/NR3C3 antibody was used at 10 ug/ml dilution of T47d and MCF-7 lysates(s) respectively.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33325AF350
Alexa Fluor 405	NBP2-33325AF405
Alexa Fluor 488	NBP2-33325AF488
Alexa Fluor 532	NBP2-33325AF532
Alexa Fluor 594	NBP2-33325AF594
Alexa Fluor 647	NBP2-33325AF647
Alexa Fluor 700	NBP2-33325AF700
Alexa Fluor 750	NBP2-33325AF750
Biotin	NBP2-33325B
DyLight 350	NBP2-33325UV
DyLight 405	NBP2-33325V
DyLight 488	NBP2-33325G
DyLight 550	NBP2-33325R
DyLight 594	NBP2-33325DL594
DyLight 650	NBP2-33325C
DyLight 680	NBP2-33325FR
DyLight 755	NBP2-33325IR
FITC	NBP2-33325F
HRP	NBP2-33325H
Janelia Fluor 549	NBP2-33325JF549
Janelia Fluor 646	NBP2-33325JF646

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-44916

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