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22 Results for "novus"
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AF-242-PB

TGF-beta RIII Antibody [Unconjugated]

(18 Publications)
250 μg
AF-242-PB
TGF‑ beta RIII was detected in immersion fixed paraffin-embedded sections of human placenta tissue using Goat Anti-Human TGF‑ beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on syncytiotrophoblast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Hu
Species Glossary
Reactivity: Hu
WB, Flow, IHC, CyTOF-ready
Applications: WB, Flow, IHC, CyTOF-ready
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 AF-242-PBU-100UG
Alexa Fluor 405 AF-242-PBV-100UG
Alexa Fluor 488 AF-242-PBG-100UG
Alexa Fluor 532 AF-242-PBX-100UG
Alexa Fluor 594 AF-242-PBT-100UG
Alexa Fluor 647 AF-242-PBR-100UG
Alexa Fluor 700 AF-242-PBN-100UG
Alexa Fluor 750 AF-242-PBS-100UG
Allophycocyanin AF-242-PBA
Biotin BAF242
CoraFluor 1 AF-242-PBCL1
DyLight 350 AF-242-PBD
DyLight 405 AF-242-PBE
DyLight 488 AF-242-PBK
DyLight 550 AF-242-PBL
DyLight 594 AF-242-PBM
DyLight 650 AF-242-PBW
DyLight 680 AF-242-PBY
DyLight 755 AF-242-PBZ
HRP AF-242-PBH
Janelia Fluor 525 AF-242-PBJF525
Janelia Fluor 549 AF-242-PBI
Janelia Fluor 646 AF-242-PBJ
Janelia Fluor 669 AF-242-PBJF669
PerCP AF-242-PBC
Phycoerythrin FAB242P
mFluor Violet 450 SE AF-242-PBMFV450
mFluor Violet 500 SE AF-242-PBMFV500
mFluor Violet 610 SE AF-242-PBMFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free FAB242P
Carrier Free AF-242-SP
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NBP1-89988

TGF-beta RIII Antibody - BSA Free

(4 Publications)
0.1 ml
NBP1-89988
Staining of human testis shows strong membranous positivity in Leydig cells.
Hu
Species Glossary
Reactivity: Hu
IHC
Applications: IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
BSA Free NBP1-89988-25ul
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AF5034

TGF-beta RIII Antibody [Unconjugated]

(2 Publications)
100 μg
AF5034
MicroRNA (miRNA) 466a-3p transfection inhibits regulatory T cell (Treg) polarization. Purified naïve CD4+ T cells were cultured under Treg-polarizing conditions along with the indicated mimic, control, or inhibitor conditions. Cells were harvested 48 h after addition of cytokines and miRNA mimics, inhibitors, or controls and subject to flow cytometry, immunoblot and quantitative real-time-PCR. The success of Treg polarization is examined as (A) representative dot plots gated on CD25HI cells and quantified in (B,C). Representative immunoblots of indicated proteins are presented in (D,F), along with associated densitometric measurements of transforming growth factor-beta 2 (TGF-beta 2) and TGF-beta R3 (E), and quantification of activated Smad 2, 3, and 4 (G). CD4+ cells were purified from naïve mouse lymph nodes and stimulated ex vivo with CD3 (3 µg/mL) and CD28 (3 µg/mL) for 48 h and administered Locked Nucleic Acid or controls at the time of seeding. Quantification of flow cytometry data from LAP-expressing FoxP3 positive Treg cells. (H) Purified naïve CD4+ T cells were cultured with either TGF-beta 1 (5 ng/mL) or TGF-beta 2 (5 ng/mL), along with CD3 (3 µg/mL), CD28 (3 µg/mL), and IL-2 (5 ng/mL) for 5 days. (I) representative dot plots of FoxP3, CD4-positive Tregs gated on CD25HI, (J), and their associated CD278 (ICOS) expression. Data are presented as mean ± SEM of three independent transfection experiments. *P < 0.05, **P < 0.005, ****P < 0.0001 by ANOVA with Tukey’s multiple comparison test. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2018.00688/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 AF5034U-100UG
Alexa Fluor 405 AF5034V-100UG
Alexa Fluor 488 AF5034G-100UG
Alexa Fluor 532 AF5034X-100UG
Alexa Fluor 594 AF5034T-100UG
Alexa Fluor 647 AF5034R-100UG
Alexa Fluor 700 AF5034N-100UG
Alexa Fluor 750 AF5034S-100UG
Allophycocyanin AF5034A
Biotin BAF5034
CoraFluor 1 AF5034CL1
DyLight 350 AF5034D
DyLight 405 AF5034E
DyLight 488 AF5034K
DyLight 550 AF5034L
DyLight 594 AF5034M
DyLight 650 AF5034W
DyLight 680 AF5034Y
DyLight 755 AF5034Z
FITC AF5034F
HRP AF5034H
Janelia Fluor 525 AF5034JF525
Janelia Fluor 549 AF5034I
Janelia Fluor 646 AF5034J
Janelia Fluor 669 AF5034JF669
PE AF5034P
PerCP AF5034C
mFluor Violet 450 SE AF5034MFV450
mFluor Violet 500 SE AF5034MFV500
mFluor Violet 610 SE AF5034MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF5034-SP
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NBP2-66592

TGF-beta RIII Antibody - BSA Free

250 μg
NBP2-66592
Immunohistochemistry: TGF-beta RIII Antibody [NBP2-66592] - extraembryonic membranes from stage 16 chick embryos using TGFIII receptor antibody.
Ch
Species Glossary
Reactivity: Ch
WB, IHC, IP
Applications: WB, IHC, IP
Sheep Polyclonal
Host: Sheep Polyclonal
Unconjugated  
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NBP2-80148

TGF-beta RIII Antibody

0.1 mg
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Goat Polyclonal
Host: Goat Polyclonal
Unconjugated  
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BAF5034

TGF-beta RIII Antibody [Biotin]

50 μg
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Goat Polyclonal
Host: Goat Polyclonal
Biotin  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF5034
Carrier Free AF5034-SP
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AF-242-PBX

TGF-beta RIII Antibody [Alexa Fluor® 532]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, Flow, IHC, CyTOF-ready
Applications: WB, Flow, IHC, CyTOF-ready
Alexa Fluor 532  
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AF-242-PBT

TGF-beta RIII Antibody [Alexa Fluor® 594]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, Flow, IHC, CyTOF-ready
Applications: WB, Flow, IHC, CyTOF-ready
Alexa Fluor 594  
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AF-242-PBG

TGF-beta RIII Antibody [Alexa Fluor® 488]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, Flow, IHC, CyTOF-ready
Applications: WB, Flow, IHC, CyTOF-ready
Alexa Fluor 488  
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AF-242-PBS

TGF-beta RIII Antibody [Alexa Fluor® 750]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, Flow, IHC, CyTOF-ready
Applications: WB, Flow, IHC, CyTOF-ready
Alexa Fluor 750  
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AF-242-PBU

TGF-beta RIII Antibody [Alexa Fluor® 350]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, Flow, IHC, CyTOF-ready
Applications: WB, Flow, IHC, CyTOF-ready
Alexa Fluor 350  
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AF-242-PBR

TGF-beta RIII Antibody [Alexa Fluor® 647]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, Flow, IHC, CyTOF-ready
Applications: WB, Flow, IHC, CyTOF-ready
Alexa Fluor 647  
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