| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13888SS | |||
| Azide and BSA Free | NBP2-80699 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56521SS | |||
| Azide and BSA Free | NBP2-33216 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB63151-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85961-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6315-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6315-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08483 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08483 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08483 | |||
![Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Western-Blot-NB120-13888-img0020.jpg "Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Ntera2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-DNMT3A (68B1446) [NB120-13888] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Immunocytochemistry-Immunofluorescence-NB120-13888-img0016.jpg)
![Immunohistochemistry-Paraffin: DNMT3A Antibody (64B1446) [NB120-13888] - Detection of DNMT3A on human hepatocellular carcinoma tissue section (NBP2-30221) using 1:100 dilution of DNMT3A antibody (clone 64B1446). The antibody generated a specific cytoplasmic staining in all the cancer cells while some of the cells depicted nuclear staining also.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Immunohistochemistry-Paraffin-NB120-13888-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with neuron-specific tubulin (green) throughout the development of the olfactory epithelium. Data courtesy of A. Jane Roskams, University of British Columbia.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Immunocytochemistry-Immunofluorescence-NB120-13888-img0017.jpg)
![Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Flow-Cytometry-NB120-13888-img0018.jpg)
![Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Using the PE direct conjugate, an intracellular stain was performed on NTERA-2 cells with DNMT3A (64B1446) NB120-13888PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Flow-Cytometry-NB120-13888-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - DNMT1 reveals a SCMC-like cortical localization with aberrant localization in Nlrp2tm1a/tm1a derived oocytes. Whole mount immunofluorescence for DNMT3A in unfertilized control oocytes reveals a characteristic metaphase associated localization and no difference is noted in oocytes derived from Nlrp2tm1a/tm1adams. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep44667), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Immunocytochemistry-Immunofluorescence-NB120-13888-img0028.jpg)
![Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on Jurkat cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Flow-Cytometry-NB120-13888-img0026.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - NTERA-2 cells were treated with Zebularine as indicated for 24 hours. Cell lysates were prepared and separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was probed with 2 ug/mL anti-Dnmt3a in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the decrease in Dnmt3a expression upon treatment with 500 uM Zebularine (arrowhead). Additional bands at 90 and 75 kDa can also be detected with this antibody and may represent alternative splice variants. Tubulin is shown as a loading control.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Western-Blot-NB120-13888-img0015.jpg)
![Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin (APC).](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Flow-Cytometry-NB120-13888-img0023.jpg)
![Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Flow-Cytometry-NB120-13888-img0024.jpg)
![Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HeLa cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin (APC).](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Flow-Cytometry-NB120-13888-img0025.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DNMT3A Antibody (64B1446) - BSA Free [NB120-13888] - DNMT3A knockdown increases miR‐200b expression via promoter demethylation. Western blot assay of DNMT3A expression in MDA‐MB‐231 and BT549 cells after transfection with DNMT3A siRNA (si‐DNMT3A) or negative control (NC). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30324719/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb120-13888_mouse-monoclonal-dnmt3a-antibody-64b1446-20920231721153.jpg)
![Flow Cytometry: DNMT3A Antibody (64B1446) - BSA Free [NB120-13888] - Protein levels of Dnmt3a & MeCP2 are reduced in inhibitory neurons of conditional knockout mice.(A) Western blot of half brain hemisphere from Dnmt3a or Mecp2 cKO mice demonstrating loss of each protein. (B) Immunofluorescence (IF) images of WT, Dnmt3a cKO or Mecp2 cKO mice probing for Dnmt3a or MeCP2 (red), the Sun1-sfGFP-myc-PA fusion protein that marks the nuclear envelope & is dependent on Cre expression (green), & DAPI to mark genomic DNA (blue). n = 3 mice per genotype (western), n = 3 mice (IF) representative images for two brain regions shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32159514), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-13888_mouse-monoclonal-dnmt3a-antibody-64b1446-310202415382413.jpg)
![Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B814-1-Western-Blot-NB100-56521-img0005.jpg "Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.")
![Flow (Intracellular): DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-56521. An intracellular stain was performed on HeLa cells with DNMT3A antibody (64B814.1) NB100-56521AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange).](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B814-1-Flow-Intracellular-NB100-56521-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: DNMT3A Antibody [NBP1-85961] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85961-img0040.jpg "Immunocytochemistry/Immunofluorescence: DNMT3A Antibody [NBP1-85961] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: DNMT3A Antibody [NBP1-85961] - Staining of human liver shows strong nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-Immunohistochemistry-Paraffin-NBP1-85961-img0044.jpg)
![Immunohistochemistry-Paraffin: DNMT3A Antibody [NBP1-85961] - Staining of human lymph node shows strong nuclear positivity.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-Immunohistochemistry-Paraffin-NBP1-85961-img0041.jpg)
![Immunohistochemistry-Paraffin: DNMT3A Antibody [NBP1-85961] - Staining of human endometrium shows strong nuclear positivity.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-Immunohistochemistry-Paraffin-NBP1-85961-img0042.jpg)
![Immunohistochemistry-Paraffin: DNMT3A Antibody [NBP1-85961] - Staining of human kidney shows nuclear positivity in cells in glomeruli and tubular cells.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-Immunohistochemistry-Paraffin-NBP1-85961-img0043.jpg)
![Immunohistochemistry-Paraffin: DNMT3A Antibody (PCRP-DNMT3A-1E2) [NBP3-07672] - Formalin-fixed, paraffin-embedded human Placenta stained with DNMT3A Mouse Monoclonal Antibody (PCRP-DNMT3A-1E2).](http://images.novusbio.com/fullsize/DNMT3A-Antibody-PCRP-DNMT3A-1E2-Immunohistochemistry-Paraffin-NBP3-07672-img0003.jpg "Immunohistochemistry-Paraffin: DNMT3A Antibody (PCRP-DNMT3A-1E2) [NBP3-07672] - Formalin-fixed, paraffin-embedded human Placenta stained with DNMT3A Mouse Monoclonal Antibody (PCRP-DNMT3A-1E2).")
![Immunohistochemistry-Paraffin: DNMT3A Antibody (PCRP-DNMT3A-1E2) [NBP3-07672] - Formalin-fixed, paraffin-embedded human Placenta stained with DNMT3A Mouse Monoclonal Antibody (PCRP-DNMT3A-1E2).](http://images.novusbio.com/fullsize/DNMT3A-Antibody-PCRP-DNMT3A-1E2-Immunohistochemistry-Paraffin-NBP3-07672-img0002.jpg)
![Protein Array: DNMT3A Antibody (PCRP-DNMT3A-1E2) [NBP3-07672] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DNMT3A Mouse Monoclonal Antibody (PCRP-DNMT3A-1E2).](http://images.novusbio.com/fullsize/DNMT3A-Antibody-PCRP-DNMT3A-1E2-Protein-Array-NBP3-07672-img0001.jpg)
![Immunoprecipitation: DNMT3A Antibody (7L9O3) [NBP3-15844] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug DNMT3A antibody (NBP3-15844). Western blot was performed from the immunoprecipitate using DNMT3A antibody (NBP3-15844) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-7L9O3-Immunoprecipitation-NBP3-15844-img0003.jpg "Immunoprecipitation: DNMT3A Antibody (7L9O3) [NBP3-15844] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug DNMT3A antibody (NBP3-15844). Western blot was performed from the immunoprecipitate using DNMT3A antibody (NBP3-15844) at a dilition of 1:1000.")
![<b>Genetic Strategies Validation. </b>Western Blot: DNMT3A Antibody (7L9O3) [DNMT3A] - Western blot analysis of lysates from wild type (WT) and DNMT3A knockout (KO) 293T cells using [KO Validated] DNMT3A at 1:500 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15844_rabbit-dnmt3a-mab-7l9o3-52202517184582.jpg)
![Western Blot: DNMT3A Antibody (7L9O3) [DNMT3A] - Western blot analysis of lysates from Mouse Thymus using DNMT3A Rabbit mAb at 1:500 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15844_rabbit-dnmt3a-mab-7l9o3-52202517202980.jpg)