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![Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-Antibody-Western-Blot-NBP2-54610-img0003.jpg "Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunohistochemistry: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] Antibody [NBP2-54610] - HeLa cells were stained with the antibody against H3K4me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K4me3 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-Antibody-Immunohistochemistry-NBP2-54610-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] [Trimethyl Lys4] Antibody [NBP2-54610] - ChIP assays were performed using human HeLa cells, the antibody against H3K4me3, and optimized PCR primer pairs for qPCR. Figure shows the recovery, expressed as a percent of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-3-Tryimethyl-Lys4-Trimethyl-Lys4-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54610-img0005.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] Antibody [NBP2-54610] - One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-Antibody-Dot-Blot-NBP2-54610-img0002.jpg)
![ELISA: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] [Trimethyl Lys4] Antibody [NBP2-54610] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K4me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:18,350.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-[Trimethyl-Lys4]-Antibody-ELISA-NBP2-54610-img0006.jpg)
![Protein Array: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] [Trimethyl Lys4] Antibody [NBP2-54610] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity for the peptides containing the H3K4me3 modification.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-[Trimethyl-Lys4]-Antibody-Protein-Array-NBP2-54610-img0007.jpg)
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3.1, H3.3 and H4 (lane 3, 4, 5, 6 and 7, respectively) using the antibody against H3K4me3. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Western-Blot-NBP2-59186-img0002.jpg "Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3.1, H3.3 and H4 (lane 3, 4, 5, 6 and 7, respectively) using the antibody against H3K4me3. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - HeLa cells were stained with the antibody against H3K4me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K4me3 antibody (left) diluted 1:100 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59186-img0001.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3 modifications and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me3 modification. At higher concentrations some H3K4me2 is also precipitated.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59186-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - ChIP assays were performed using human HeLa cells, the Ab against H3K4me3 and PCR primers for qPCR. ChIP was performed using sheared chromatin from 1 million cells. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active GAPDH and EIF4A2 genes, used as positive controls, and for the inactive MYOD1 gene, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of IP DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59186-img0004.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K4me3 with peptides containing other modifications and unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Dot-Blot-NBP2-59186-img0003.jpg)
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody (C.15200152) [NBP2-59289] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys4-Antibody-C-15200152-Western-Blot-NBP2-59289-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys4] Antibody (C.15200152) [NBP2-59289] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys4] Antibody (C.15200152) [NBP2-59289] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys4-Antibody-C-15200152-Immunofluorescence-NBP2-59289-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody (C.15200152) [NBP2-59289] - ChIP assays were performed using human HeLa cells, the antibody against H3K4me3 and PCR primer pairs for qPCR. ChIP was performed with a Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the constitutively expressed GAPDH and c-fos genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys4-Antibody-C-15200152-Chromatin-Immunoprecipitation-NBP2-59289-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys4] Antibody (C.15200152) [NBP2-59289] - Cross reactivity of the antibody directed against H3K4me3. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K4me3. The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys4-Antibody-C-15200152-ELISA-NBP2-59289-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP3-25986] - A Peptide dotblot shows RM137 reacts only to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).](http://images.novusbio.com/fullsize/nbp3-25986_rabbit-histone-h3-mab-rm137-azide-and-bsa-free-24620241332444.png "Dot Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP3-25986] - A Peptide dotblot shows RM137 reacts only to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).")
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP3-25986] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM137 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 4 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25986_rabbit-histone-h3-mab-rm137-azide-and-bsa-free-24620241332426.png)
![Multiplex Immunoassay: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP3-25986] - RM137 specifically reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25986_rabbit-histone-h3-mab-rm137-azide-and-bsa-free-2462024132622.png)
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) [NBP2-61502] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61502 at 0.5 ug/ml, showed a band of histone H3 trimethylated at Lysine 4 (K4me3) in HeLa cells. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys4-Antibody-RM137-Western-Blot-NBP2-61502-img0003.jpg "Western Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) [NBP2-61502] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61502 at 0.5 ug/ml, showed a band of histone H3 trimethylated at Lysine 4 (K4me3) in HeLa cells. Observed molecular weight is ~17 kDa.")
![Multiplex Immunoassay: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP2-61502] - RM137 specifically reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/nbp2-61502_rabbit-monoclonal-histone-h3-trimethyl-lys4-antibody-rm137-multiplex-immunoassay-18920252352114.png)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP2-61502] - A Peptide dotblot shows RM137 reacts only to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).](http://images.novusbio.com/fullsize/nbp2-61502_rabbit-monoclonal-histone-h3-trimethyl-lys4-antibody-rm137-dot-blot-18920252342352.png)