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![Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Western-Blot-NB300-118-img0001.jpg "Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.")
![Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - The blots show the examples of immunobands against NMDAR1 and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Western-Blot-NB300-118-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Immunocytochemistry-Immunofluorescence-NB300-118-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mouse neuron stained with anti-NMDAR1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Immunocytochemistry-Immunofluorescence-NB300-118-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR1 (1:200) and anti-mouse CF568 (1:250). The antibody labels small punctae along the neuronal processes. No distinct features such as spines are visible. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Immunocytochemistry-Immunofluorescence-NB300-118-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118] - Notch1 colocalizes postsynaptically with Reelin signaling components. (A) Representative IEM images from hippocampal slices using an antibody specific for Notch1 show that gold particles are localized at postsynaptic as well as presynaptic membrane terminals. (B) Bar graph summarizing the counting of gold particles on the length of presynaptic & postsynaptic membranes indicates that the majority of the Notch1 gold particles are localized postsynaptically (5*10−3 ± 0.8*10−3 vs. 2*10−3 ± 0.4*10−3, n = 3 mice; Student’s t-test, p < 0.01). (C) Fluorescent immunolabeling on 14 days primary neuronal WT cultures shows colocalization of Notch1 & ApoER2 in soma & processes of pyramidal neurons. Both Notch1 & ApoER2 are similarly co-expressed in NMDAR1 positive puncta (R = 0.92 ± 0.03 & R = 0.86 ± 0.08, respectively; Student’s t-test, p = 0.23). (C′) Close up of a dendrite displaying Notch1, ApoER2 & NMDAR1 expression & “zoom in” captions of puncta showing clustering of the three receptors in teal. (D) Fluorescent immunolabeling on primary neuronal cultures shows that Notch1 & Dab1 localize in the same pyramidal neuron’s soma & processes labeled by phalloidin (R = 0.87 ± 0.06 & R = 0.74 ± 0.07 respectively; Student’s t-test, p = 0.5). (D′) Close up of a dendrite with Notch1, Dab1 & F-actin labeling & “zoom in” captions of dendritic puncta showing clustering of Notch1, Dab1 & F-actin in teal. **p < 0.01. Error bars are SEM & scale bar in (A) is 200 nm for all IEM panels, in (C,D) 50 μm & in (C′,D′) 10 μm & 500 nm in zoom in captions. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26635527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-118_mouse-monoclonal-nmdar1-antibody-r1jhl-imgenex-img-5000-31020241620518.jpg)
![Western Blot: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control: Line 1: MCF-7 Line 2: A549](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Western-Blot-NBP2-66982-img0004.jpg "Western Blot: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control: Line 1: MCF-7 Line 2: A549")
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Staining NMDAR1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunocytochemistry-Immunofluorescence-NBP2-66982-img0005.jpg)
![Immunohistochemistry-Paraffin: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of paraffin-embedded rat brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunohistochemistry-Paraffin-NBP2-66982-img0003.jpg)
![Flow Cytometry: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of SH-SY5Y cells with NMDAR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Flow-Cytometry-NBP2-66982-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Staining NMDAR1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunocytochemistry-Immunofluorescence-NBP2-66982-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Staining NMDAR1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunocytochemistry-Immunofluorescence-NBP2-66982-img0006.jpg)
![Immunohistochemistry-Paraffin: NMDAR1 Antibody (JM11-26) [NBP2-66982] - Analysis of paraffin-embedded mouse brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-JM11-26-Immunohistochemistry-Paraffin-NBP2-66982-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR1 stained by NMDAR1 antibody (NBP3-13158) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13158-img0005.jpg "Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR1 stained by NMDAR1 antibody (NBP3-13158) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Frozen: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: NMDAR1 protein stained by NMDAR1 antibody (NBP3-13158) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-Immunohistochemistry-Frozen-NBP3-13158-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein at synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR1 protein stained by NMDAR1 antibody (NBP3-13158) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue:Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13158-img0003.jpg)
![Immunohistochemistry-Paraffin: NMDAR1 Antibody [NBP3-13158] - Rat brain. NMDAR1 antibody (NBP3-13158) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/NMDAR1-Antibody-Immunohistochemistry-Paraffin-NBP3-13158-img0001.jpg)
![Western Blot: NMDAR1 Antibody [NBP3-13158] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NMDAR1 antibody (NBP3-13158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13158_rabbit-nmdar1-pab-91020242041289.jpg)
![Western Blot: NMDA receptor N1 Antibody [NB300-114] - 10 ug of HEK 293 cells expressing: Lane 1 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the N1 splice variant insert.; Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the C1 and C2' Insert; Lane 4 - without NR1 expression (Mock).](http://images.novusbio.com/fullsize/NMDA-receptor-N1-Antibody-Western-Blot-NB300-114-img0004.jpg "Western Blot: NMDA receptor N1 Antibody [NB300-114] - 10 ug of HEK 293 cells expressing: Lane 1 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the N1 splice variant insert.; Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the C1 and C2' Insert; Lane 4 - without NR1 expression (Mock).")
![Flow Cytometry: NMDA receptor N1 Antibody [NB300-114] - CD4+ Lymphocytes expressing NMDAR1. Image provided by verified customer.](http://images.novusbio.com/fullsize/NMDA-receptor-N1-Antibody-Flow-Cytometry-NB300-114-img0002.jpg)
![Western Blot: NMDAR1 Antibody (1H1T3) [NBP3-15431] - Western blot analysis of extracts of Mouse brain, using NMDAR1 Rabbit mAb (NBP3-15431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-1H1T3-Western-Blot-NBP3-15431-img0004.jpg "Western Blot: NMDAR1 Antibody (1H1T3) [NBP3-15431] - Western blot analysis of extracts of Mouse brain, using NMDAR1 Rabbit mAb (NBP3-15431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (1H1T3) [NBP3-15431] -Confocal imaging of Mouse brain using NMDAR1 Rabbit mAb (dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-15431_rabbit-nmdar1-mab-1h1t3-immunocytochemistry-immunofluorescence-11120241352..jpg)
![Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (1H1T3) [NMDAR1] - Immunofluorescence analysis of paraffin-embedded Mouse brain tissue using NMDAR1 Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15431_rabbit-nmdar1-mab-1h1t3-52202517162381.jpg)
![Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (1H1T3) [NMDAR1] - Immunofluorescence analysis of paraffin-embedded Rat brain tissue using NMDAR1 Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15431_rabbit-nmdar1-mab-1h1t3-52202517172882.jpg)
![Western Blot: NMDAR1 Antibody (S308-48) [NBP2-59327] - Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone S308-48 (NBP2-59327). Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody (NBP2-59327) at 1:1000.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-S308-48-Western-Blot-NBP2-59327-img0004.jpg "Western Blot: NMDAR1 Antibody (S308-48) [NBP2-59327] - Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone S308-48 (NBP2-59327). Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody (NBP2-59327) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (S308-48) [NBP2-59327] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NMDAR1/NR1 Monoclonal Antibody, Clone S308-48 (NBP2-59327). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-NMDAR1/NR1 Monoclonal Antibody (NBP2-59327) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) NMDAR1/NR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-S308-48-Immunocytochemistry-Immunofluorescence-NBP2-59327-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (S308-48) [NBP2-59327] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN1/NR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GluN1/NR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-S308-48-Immunocytochemistry-Immunofluorescence-NBP2-59327-img0003.jpg)
![Western Blot: NMDA receptor C1 Antibody [NB300-115] - 10 ug of HEK 293 cells expressing: Lane 1 - HEK cells without NR1 expression (Mock); Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the C1 and C2' Insert; Lane 4 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the C1 splice variant insert.](http://images.novusbio.com/fullsize/NMDA-receptor-C1-Antibody-Western-Blot-NB300-115-img0002.jpg "Western Blot: NMDA receptor C1 Antibody [NB300-115] - 10 ug of HEK 293 cells expressing: Lane 1 - HEK cells without NR1 expression (Mock); Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the C1 and C2' Insert; Lane 4 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the C1 splice variant insert.")
![Western Blot: NMDAR2C Splice Variant Antibody [NB300-116] - Western blot of 10 ug of HEK 293 cells expression. (Lane A) NR1-subunit that does not contain the NR1 Insert (Mock). (Lane B) NR1-subunit containing only the C2 Insert. (Lane C) NR1-subunit containing the C1 and C2 in sert. (Lane D) NR1-subunit containing the C1/C2' and N1/C2' insert.](http://images.novusbio.com/fullsize/NMDAR2C-Splice-Variant-Antibody-Western-Blot-NB300-116-img0003.jpg "Western Blot: NMDAR2C Splice Variant Antibody [NB300-116] - Western blot of 10 ug of HEK 293 cells expression. (Lane A) NR1-subunit that does not contain the NR1 Insert (Mock). (Lane B) NR1-subunit containing only the C2 Insert. (Lane C) NR1-subunit containing the C1 and C2 in sert. (Lane D) NR1-subunit containing the C1/C2' and N1/C2' insert.")
![Western Blot: NMDAR2C Prime Splice Variant Antibody [NB300-117] - Western blot of 10 ug of HEK 293 cells expression. (Lane A) NR1-subunit that does not contain the NR1 Insert (Mock). (Lane B) NR1-subunit containing only the C2 Insert. (Lane C) NR1-subunit containing the C1 and C2 insert. (Lane D) NR1-subunit containing the C11/C2' and N1/C2' insert.](http://images.novusbio.com/fullsize/NMDAR2C-Prime-Splice-Variant-Antibody-Western-Blot-NB300-117-img0002.jpg "Western Blot: NMDAR2C Prime Splice Variant Antibody [NB300-117] - Western blot of 10 ug of HEK 293 cells expression. (Lane A) NR1-subunit that does not contain the NR1 Insert (Mock). (Lane B) NR1-subunit containing only the C2 Insert. (Lane C) NR1-subunit containing the C1 and C2 insert. (Lane D) NR1-subunit containing the C11/C2' and N1/C2' insert.")
![Western Blot: NMDAR1 [p Ser896] Antibody [NB100-82092] - Analysis of extracts from Mouse Brain tissue using NMDAR1(Phospho-Ser896) Antibody.](http://images.novusbio.com/fullsize/NMDAR1-[p-Ser896]-Antibody-Western-Blot-NB100-82092-img0002.jpg "Western Blot: NMDAR1 [p Ser896] Antibody [NB100-82092] - Analysis of extracts from Mouse Brain tissue using NMDAR1(Phospho-Ser896) Antibody.")