| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6570-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98877-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92937-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98894-50ul | |||
![Western Blot: Cyclin D3 Antibody [NBP2-98877] - Anti-Cyclin D3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-Western-Blot-NBP2-98877-img0002.jpg "Western Blot: Cyclin D3 Antibody [NBP2-98877] - Anti-Cyclin D3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa")
![Immunocytochemistry/Immunofluorescence: Cyclin D3 Antibody [NBP2-98877] - Immunofluorescence staining of Cyclin D3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98877 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98877_rabbit-polyclonal-cyclin-d3-antibody-23520251125446.png)
![Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of various cell lines, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-4C5C8-Western-Blot-NBP3-16309-img0001.jpg "Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of various cell lines, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of Rat lung, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-4C5C8-Western-Blot-NBP3-16309-img0002.jpg)
![Western Blot: Cyclin D3 Antibody [NBP2-92937] - Analysis of extracts of various cell lines, using Cyclin D3 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-Western-Blot-NBP2-92937-img0002.jpg "Western Blot: Cyclin D3 Antibody [NBP2-92937] - Analysis of extracts of various cell lines, using Cyclin D3 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclin D3 Antibody [NBP2-92937] - Analysis of extracts from normal (control) and CCND3 knockout (KO) HeLa cells, using Cyclin D3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-Knockout-Validated-NBP2-92937-img0001.jpg)
![Western Blot: Cyclin D3 Antibody [NBP2-98894] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:35 kDa](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-Western-Blot-NBP2-98894-img0002.jpg "Western Blot: Cyclin D3 Antibody [NBP2-98894] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:35 kDa")
![Immunoprecipitation: Cyclin D3 Antibody [NBP2-98894] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-CCND3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-CCND3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 36 kDa](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-Immunoprecipitation-NBP2-98894-img0001.jpg)
![Western Blot: Cyclin D3 Overexpression Lysate (Adult Normal) [NBP2-04203] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cyclin D3.](http://images.novusbio.com/fullsize/Cyclin-D3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04203-img0001.jpg "Western Blot: Cyclin D3 Overexpression Lysate (Adult Normal) [NBP2-04203] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cyclin D3.")