| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-75925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-24867 | |||
| BSA Free | NBP2-25186SS | |||
| Low Endotoxin, Azide and BSA Free | NBP2-24867SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NB600-1443-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NB120-10842-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-62230 | |||
![Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Western-Blot-NBP2-75925-img0002.jpg "Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Analysis of a FFPE tissue section of the human intesting using 1:200 dilution of TNF-alpha antibody (NBP2-75925). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a moderate to strong cytoplasmic staining in crypt cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-Paraffin-NBP2-75925-img0003.jpg)
![Neutralization: TNF-alpha Antibody (2342C) [NBP2-75925] - Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # NBP2-75925). The ND50 is typically 1-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Neutralization-NBP2-75925-img0001.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Alexa Fluor (R) 488 conjugate of NBP2-24867. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0009.jpg "Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Alexa Fluor (R) 488 conjugate of NBP2-24867. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.")
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using the Alexa Fluor(R) 700 conjugate of NBP2-24867. Staining of human PBMC. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0008.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Azide Free version of NBP2-25186. CD3+ hPMBCs using the APC conjugate of this antibody. A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched isotype control](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0005.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis of CD3 expression in hPBMC's gated to lymphocyte population at 1 x 10^6 cells/mL. Cells were stained with NBP2-24867PE (orange) conjugate at a 1:500 dilution. Results are shown with mIgG (PE conjugate) isotype control (blue). Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0013.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis of PerCP conjugate of NBP2-25186. A surface stain was performed on Jurkat cells with CD3 (OKT3) antibody NBP2-24867PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0004.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using the Alexa Fluor(R) 488 conjugate of NBP2-24867. Detection of CD3 expression in hPBMc's gated to lymphocyte population at 1 x 10^6 cells/mL. Cells were stained with NBP2-24867AF488 (orange) conjugate at a 1:500 dilution. Results are shown with mIgG2 (AF488 conjugate) isotype control (blue). Image using the Low Endotoxin and Azide Free form of this antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0011.jpg)
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in HCa-1 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0003.jpg "Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in HCa-1 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.")
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. HCa-1 and MIH-2 cells were injected intramuscularly into the right thighs of the mice, and tumors were irradiated with 10 Gy of radiation. Shown are IL-12 and DC (CD11c+) expressions at 1, 3, and 7 days after irradiation in HCa-1 tumors in the spleens of HCa-1 bearing mice (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0001.jpg)
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. HCa-1 and MIH-2 cells were injected intramuscularly into the right thighs of the mice, and tumors were irradiated with 10 Gy of radiation. Shown are IL-12 and DC (CD11c+) expressions at 1, 3, and 7 days after irradiation in MIH-2 tumors in the spleens of HCa-1 bearing mice (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0002.jpg)
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro.IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.HCa-1 & MIH-2 cells were injected intramuscularly into the right thighs of the mice, & tumors were irradiated with 10 Gy of radiation. Shown are IL-12 & DC (CD11c+) expressions at 1, 3, & 7 days after irradiation in (a) HCa-1 & (b) MIH-2 tumors & (c) IL-12 expression in the spleens of HCa-1 bearing mice (** P < 0.01). (d) IL-12 expression in BMDCs incubated with supernatant of MIH-2 tumor cells or supernatant of 10 Gy irradiating MIH-2 tumor cells. (** P < 0.01). DCs were differentiated from bone marrow of C3H/HeN normal mice (BMDCs). These were incubated for 72 h in supernatant of MIH-2 tumor cells with or without 10 Gy irradiation. IL-12 was intracellular stained with IL-12 antibody & analyzed by FACS. BMDCs also were stimulated with LPS (100 ng/mL) to allow maturation & irradiated with 10 Gy of radiation. (e) IL-12 expression (** P < 0.01), (f) cell viability, & (g) B7.1 expression in LPS stimulated BMDCs receiving 10 Gy radiation. (* P < 0.05). Data are from three independent experiments with five mice per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-31020241616187.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.IL-6 & IL-12 were measured at 1, 3, & 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 & IL-12 in (a) HCa-1 & (b) MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice & stimulated with LPS. (c) IL-6 expression analyzed at the indicated times by ELISA in irradiated or non-irradiated BMDCs in vitro. (d) IL-6R (** P < 0.01, vs No IR) & (e) sIL6R (** P < 0.01) were evaluated at 2 day after 10 Gy irradiation to BMDCs by FACS & ELISA, respectively. BMDCs were treated without or with IL-6 (20 ng/mL) plus LPS. (f) Down regulation of IL-12 production by IL-6 treatment in BMDCs (** P < 0.01). BMDCs were stimulated with LPS for activation & cultured with or without anti-IL-6 antibody (final concentration of 500 ng/mL) during the indicated times after irradiation in vitro. IL-6 & IL-12 were analyzed by ELISA. (g) Recovery of IL-12 production & (h) B7-1/ B7-2 expression using an anti-IL-6 antibody in irradiated-BMDCs stimulated with LPS in vitro. (** P < 0.01, * P < 0.05). Data are from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416182614.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.IL-6 & IL-12 were measured at 1, 3, & 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 & IL-12 in (a) HCa-1 & (b) MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice & stimulated with LPS. (c) IL-6 expression analyzed at the indicated times by ELISA in irradiated or non-irradiated BMDCs in vitro. (d) IL-6R (** P < 0.01, vs No IR) & (e) sIL6R (** P < 0.01) were evaluated at 2 day after 10 Gy irradiation to BMDCs by FACS & ELISA, respectively. BMDCs were treated without or with IL-6 (20 ng/mL) plus LPS. (f) Down regulation of IL-12 production by IL-6 treatment in BMDCs (** P < 0.01). BMDCs were stimulated with LPS for activation & cultured with or without anti-IL-6 antibody (final concentration of 500 ng/mL) during the indicated times after irradiation in vitro. IL-6 & IL-12 were analyzed by ELISA. (g) Recovery of IL-12 production & (h) B7-1/ B7-2 expression using an anti-IL-6 antibody in irradiated-BMDCs stimulated with LPS in vitro. (** P < 0.01, * P < 0.05). Data are from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416154619.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.HCa-1 & MIH-2 cells were injected intramuscularly into the right thighs of the mice, & tumors were irradiated with 10 Gy of radiation. Shown are IL-12 & DC (CD11c+) expressions at 1, 3, & 7 days after irradiation in (a) HCa-1 & (b) MIH-2 tumors & (c) IL-12 expression in the spleens of HCa-1 bearing mice (** P < 0.01). (d) IL-12 expression in BMDCs incubated with supernatant of MIH-2 tumor cells or supernatant of 10 Gy irradiating MIH-2 tumor cells. (** P < 0.01). DCs were differentiated from bone marrow of C3H/HeN normal mice (BMDCs). These were incubated for 72 h in supernatant of MIH-2 tumor cells with or without 10 Gy irradiation. IL-12 was intracellular stained with IL-12 antibody & analyzed by FACS. BMDCs also were stimulated with LPS (100 ng/mL) to allow maturation & irradiated with 10 Gy of radiation. (e) IL-12 expression (** P < 0.01), (f) cell viability, & (g) B7.1 expression in LPS stimulated BMDCs receiving 10 Gy radiation. (* P < 0.05). Data are from three independent experiments with five mice per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416163756.jpg)
![Western Blot: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-human CD43 (MEM-59).Lane 1: lysate of human PBLLane 2: lysate of canine PBLLane 3: lysate of porcine PBL](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-MEM-59-Low-Endotoxin-Western-Blot-NBP2-62227-img0005.jpg "Western Blot: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-human CD43 (MEM-59).Lane 1: lysate of human PBLLane 2: lysate of canine PBLLane 3: lysate of porcine PBL")
![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Staining of human spleen (paraffin sections) using anti-CD43 (MEM-59).](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-MEM-59-Low-Endotoxin-Immunohistochemistry-Paraffin-NBP2-62227-img0006.jpg)
![Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) PE.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-MEM-59-Low-Endotoxin-Flow-Cell-Surface-NBP2-62227-img0004.jpg)
![Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) APC-CyTM7.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-MEM-59-Low-Endotoxin-Flow-Cell-Surface-NBP2-62227-img0001.jpg)
![Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-MEM-59-Low-Endotoxin-Flow-Cell-Surface-NBP2-62227-img0002.jpg)
![Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) FITC.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-MEM-59-Low-Endotoxin-Flow-Cell-Surface-NBP2-62227-img0003.jpg)
![Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin [NBP2-62230] - Surface staining of human peripheral blood with anti-human CD300e (UP-H2) PE.](http://images.novusbio.com/fullsize/CD300e-LMIR6-Antibody-UP-H2-Low-Endotoxin-Flow-Cell-Surface-NBP2-62230-img0002.jpg "Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin [NBP2-62230] - Surface staining of human peripheral blood with anti-human CD300e (UP-H2) PE.")
![Flow (Cell Surface): CD300e/LMIR6 Antibody (UP-H2) - Low Endotoxin [NBP2-62230] - Surface staining of human peripheral blood with anti-human CD300e (UP-H2) APC.](http://images.novusbio.com/fullsize/CD300e-LMIR6-Antibody-UP-H2-Low-Endotoxin-Flow-Cell-Surface-NBP2-62230-img0001.jpg)
