| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF970-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF980-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB970-SP | |||
| Carrier Free | MAB970-500 | |||
| Carrier Free | IC970U-100UG | |||
| Carrier Free | IC970T-100UG | |||
| Carrier Free | IC970S-100UG | |||
| Carrier Free | IC970R-100UG | |||
| Carrier Free | IC970V-100UG | |||
| Carrier Free | IC970N-100UG | |||
| Carrier Free | IC970G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF580-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9801-SP | |||
| Carrier Free | MAB9801-500 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB580U-100UG | |||
| Alexa Fluor 405 | FAB580V-100UG | |||
| Alexa Fluor 488 | FAB580G-100UG | |||
| Alexa Fluor 532 | FAB580X-100UG | |||
| Alexa Fluor 594 | FAB580T-100UG | |||
| Alexa Fluor 647 | FAB580R-100UG | |||
| Alexa Fluor 700 | FAB580N-100UG | |||
| Alexa Fluor 750 | FAB580S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB580-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21717-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21705-50ul | |||
![Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis was performed on whole cell extracts (30 ug lysate) of A431 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), HT29 (Lane 4) and COS-7 (Lane 5). The blots were probed with Anti-TIMP1 Mouse .](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Western-Blot-NB100-74551-img0009.jpg "Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis was performed on whole cell extracts (30 ug lysate) of A431 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), HT29 (Lane 4) and COS-7 (Lane 5). The blots were probed with Anti-TIMP1 Mouse .")
![Immunocytochemistry/Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TIMP1 Monclonal Antibody (F31 P2 A5) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunocytochemistry-Immunofluorescence-NB100-74551-img0005.jpg)
![Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Normal biopsies of deparaffinized Human prostate tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunohistochemistry-Paraffin-NB100-74551-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis of TIMP1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TIMP1 Monclonal Antibody (F31 P2 A5) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunocytochemistry-Immunofluorescence-NB100-74551-img0004.jpg)
![Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunohistochemistry-Paraffin-NB100-74551-img0007.jpg)
![Western Blot: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Western blot analysis of TIMP-1 on different lysates with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: PANC-1 cell lysate Lane 3: SW1990 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 23 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-288202412493478.jpg "Western Blot: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Western blot analysis of TIMP-1 on different lysates with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: PANC-1 cell lysate Lane 3: SW1990 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 23 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TIMP-1 antibody (NBP3-33022) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33022) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-298202414115428.jpg)
![Flow Cytometry: TIMP-1 Antibody (JE59-72) [NBP3-33022] - Flow cytometric analysis of HT-29 cells labeling TIMP-1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33022, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33022_rabbit-timp-1-mab-je59-72-392024884693.jpg)
![Western Blot: TIMP-1 Antibody (SR1426) [NBP3-21717] - Western blot analysis using NBP3-21717 in human prostate cancer lysate.](http://images.novusbio.com/fullsize/nbp3-21717_rabbit-timp-1-mab-sr1426-5720231716185.jpg "Western Blot: TIMP-1 Antibody (SR1426) [NBP3-21717] - Western blot analysis using NBP3-21717 in human prostate cancer lysate.")
![Western Blot: TIMP-1 Antibody (SR1425) [NBP3-21705] - Western blot analysis using NBP3-21705 in HL60 cell lysate treated with TPA.](http://images.novusbio.com/fullsize/nbp3-21705_rabbit-timp-1-mab-sr1425-57202316582152.jpg "Western Blot: TIMP-1 Antibody (SR1425) [NBP3-21705] - Western blot analysis using NBP3-21705 in HL60 cell lysate treated with TPA.")