Hu,
Mu,
Rt,
Bv,
FeSpecies Glossary
Reactivity: Hu, Mu, Rt, Bv, Fe
WB, Simple Western, ELISA, ICC/IF, IHC, IP, IHC, KO, mIF, MS
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IP, IHC, KO, mIF, MS
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Immunocytochemistry/Immunofluorescence: Collagen IV Antibody [NB120-6586] - Immunofluorescence staining of MDA-MB-231 cells for collagen IV after 7 days of culture in 6 vs. 2.5mgml^-1. Representative images of n=2 biological replicates. Scale bar 100um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29162797/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-Immunocytochemistry-Immunofluorescence-NB120-6586-img0017.jpg "Immunocytochemistry/Immunofluorescence: Collagen IV Antibody [NB120-6586] - Immunofluorescence staining of MDA-MB-231 cells for collagen IV after 7 days of culture in 6 vs. 2.5mgml^-1. Representative images of n=2 biological replicates. Scale bar 100um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29162797/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-Immunohistochemistry-Paraffin-NB120-6586-img0013.jpg)
![Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis of Collagen IV in various paraffin embedded human tissues. Image courtesy of product review submitted by Daniel Pirici.](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-Immunohistochemistry-Paraffin-NB120-6586-img0006.jpg)
![Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Collagen IV antibody at 1:400, 45 min RT showed strong staining in FFPE sections of human kidney (left) with strong red staining observed in glomeruli and liver (right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 100C for 20 minutes for antigen retrieval.](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-Immunohistochemistry-Paraffin-NB120-6586-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis of Collagen IV in paraffin embedded mouse cervix tissue using anti-Collagen IV antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-Immunohistochemistry-Paraffin-NB120-6586-img0010.jpg)
![Immunohistochemistry-Paraffin: Collagen IV Antibody [NB120-6586] - Analysis using the Biotin conjugate of NB120-6586. Staining of Collagen IV (red) and Insulin (green) in mouse pancreas. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-Immunohistochemistry-Paraffin-NB120-6586-img0009.jpg)
![Immunohistochemistry-Frozen: Collagen IV Antibody [NB120-6586] - Mouse liver tissue. Collagen IV antibody at 1:200 dilution. Overnight incubation, no permeabilization. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-Immunohistochemistry-Frozen-NB120-6586-img0011.jpg)
![Immunohistochemistry-Frozen: Collagen IV Antibody [NB120-6586] - Mouse brain tissue. Antibody at 1:500. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-Immunohistochemistry-Frozen-NB120-6586-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody [NB120-6586] - Collagen IV was detected in immersion fixed THP-1 (positive) but not THP-1 KO (negative) cells using 10 ug/mL Rabbit Anti-Human/Bovine Polyclonal Antibody (Catalog # NB120-6586) for 3 hours at room temperature. Cells were stained with the NorthernLights(TM) 557-conjugated Donkey anti-Rabbit IgG Secondary Antibody [NL557] (red; Catalog # NL004) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-Knockout-Validated-NB120-6586-img0016.jpg)