| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8963-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73209 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8964-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82120-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73209 | |||
![Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Abeta facilitates HIF1alpha synthesis and autophagy inhibition via mTOR activation. Cells were incubated with rapamycin (10 nM) for 30 min prior to Abeta treatment for 24 h. Phosphorylation of 4EBP1 (Thr 37/46) and 4EBP1, phosphorylation of p70S6K1 (Thr 389), HIF1alpha and beta-actin were analyzed by western blot. n = 6. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnmol.2017.00229/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr389-412]-Antibody-Western-Blot-NB600-1049-img0005.jpg "Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Abeta facilitates HIF1alpha synthesis and autophagy inhibition via mTOR activation. Cells were incubated with rapamycin (10 nM) for 30 min prior to Abeta treatment for 24 h. Phosphorylation of 4EBP1 (Thr 37/46) and 4EBP1, phosphorylation of p70S6K1 (Thr 389), HIF1alpha and beta-actin were analyzed by western blot. n = 6. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnmol.2017.00229/full) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of Phospho-p70 S6 Kinase in 293 cells.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr389]-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1049-img0002.jpg)
![Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Human liver cancer tissue sections](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr389]-Antibody-Immunohistochemistry-NB600-1049-img0003.jpg)
![Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of phospho-p70 S6 Kinase in human breast cancer cell lines (MDA-MB-231 and MDA-MB-435) using p70 S6 Kinase/S6K [p Thr390, p Thr412] Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr389]-Antibody-Western-Blot-NB600-1049-img0001.jpg)
![Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of p70 S6 Kinase phosphorylation expression in Insulin treatedJurkat whole cell lysates. The lane on the left is treated with the antigen-specific peptide.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr389]-Antibody-Western-Blot-NB600-1049-img0004.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - Analysis of RPS6KB1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-4H4-Western-Blot-H00006198-M04-img0012.jpg "Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - Analysis of RPS6KB1 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - Analysis of monoclonal antibody to RPS6KB1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-4H4-Immunocytochemistry-Immunofluorescence-H00006198-M04-img0008.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - s6k in fibroblasts. Image from verified customer review.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-4H4-Western-Blot-H00006198-M04-img0007.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - Analysis of RPS6KB1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-4H4-Western-Blot-H00006198-M04-img0009.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - Analysis of RPS6KB1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-4H4-Western-Blot-H00006198-M04-img0010.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - Analysis of RPS6KB1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-4H4-Western-Blot-H00006198-M04-img0011.jpg)
![ELISA: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - Detection limit for recombinant GST tagged RPS6KB1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-4H4-ELISA-H00006198-M04-img0006.jpg)
![Western Blot: S6K Antibody (1G4) [NBP2-00754] Analysis of extracts (35ug) from 9 different cell lines by using anti-S6K monoclonal antibody.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Western-Blot-NBP2-00754-img0008.jpg "Western Blot: S6K Antibody (1G4) [NBP2-00754] Analysis of extracts (35ug) from 9 different cell lines by using anti-S6K monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: S6K Antibody (1G4) [NBP2-00754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY S6K.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Immunocytochemistry-Immunofluorescence-NBP2-00754-img0007.jpg)
![Immunohistochemistry-Paraffin: S6K Antibody (1G4) [NBP2-00754] - Staining of paraffin-embedded Human pancreas tissue using anti-S6K mouse monoclonal antibody.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Immunohistochemistry-Paraffin-NBP2-00754-img0006.jpg)
![Flow Cytometry: S6K Antibody (1G4) [NBP2-00754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-S6K antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Flow-Cytometry-NBP2-00754-img0002.jpg)
![Western Blot: S6K Antibody (1G4) [NBP2-00754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S6K (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S6K.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Western-Blot-NBP2-00754-img0003.jpg)
![Immunohistochemistry-Paraffin: S6K Antibody (1G4) [NBP2-00754] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-S6K mouse monoclonal antibody.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Immunohistochemistry-Paraffin-NBP2-00754-img0004.jpg)
![Immunohistochemistry-Paraffin: S6K Antibody (1G4) [NBP2-00754] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-S6K mouse monoclonal antibody.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Immunohistochemistry-Paraffin-NBP2-00754-img0005.jpg)
![Flow Cytometry: S6K Antibody (1G4) [NBP2-00754] - Analysis of Jurkat cells, using anti-S6K antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Flow-Cytometry-NBP2-00754-img0001.jpg)
![Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using NB100-82120 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Ser411]-Antibody-Immunohistochemistry-NB100-82120-img0006.jpg "Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using NB100-82120 (left) or the same antibody preincubated with blocking peptide(right).")
![<b>Biological Strategies Validation. </b>Western Blot: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Analysis of extracts from MCF cells untreated or treated with EGF using NB100-82120](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Ser411]-Antibody-Western-Blot-NB100-82120-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Staining of methanol-fixed MCF7 cells using NB100-82120.](http://images.novusbio.com/fullsize/nb100-82120_rabbit-polyclonal-p70-s6-kinase-s6k-p-ser411-antibody-11320251555919.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody [NBP2-38448] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Western-Blot-NBP2-38448-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p70 S6 Kinase/S6K Antibody [NBP2-38448] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K Antibody [NBP2-38448] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38448-img0004.jpg)
![Immunohistochemistry: p70 S6 Kinase/S6K Antibody [NBP2-38448] - Staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Immunohistochemistry-NBP2-38448-img0002.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody [NBP2-38448] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Western-Blot-NBP2-38448-img0003.jpg)
![Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32783) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-29820241492679.jpg "Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32783) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunocytochemistry analysis of MCF7 cells treated with or without 20% FBS overnight then add 100nM Calyculin A for 30 minutes labeling p70 S6 Kinase/S6K with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-292024111483.jpg)
![Western Blot: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Western blot analysis of p70 S6 Kinase/S6K on different lysates with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 59 kDa Observed band size: 70/85 kDa Exposure time: Lane 1-4 (left): 1 minute 2 seconds; Lane 1-4 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-3920248453540.jpg)
![p70 S6 Kinase/S6K Antibody (OTI1G4) [mFluor Violet 450 SE] [NBP2-73209MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.](http://images.novusbio.com/fullsize/nbp2-73209mfv450_mouse-p70-s6-kinase-s6k-mab-oti1g4-mfluor-violet-450-se-22620231330747.png "p70 S6 Kinase/S6K Antibody (OTI1G4) [mFluor Violet 450 SE] [NBP2-73209MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.")