| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-615SS | |||
| Azide and BSA Free | NBP2-80617 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40760SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37300-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59390-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54369-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22666-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26695-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21931-50ul | |||
![Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Western-Blot-NB100-615-img0010.jpg "Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.")
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or deltaCT-GFP were fixed, immunostained for caveolin-1 (1:100, Novus Biologicals), and examined by confocal fluorescence microscopy. For hPARM-1-GFP-caveolin-1 co-localization, cells that demonstrated cell membrane PARM-1 localization were chosen. All co-localizations were observed following merging images of GFP-tagged proteins with those of Golgi, endosomes, plasma membrane, alpha-tubulin or caveolin-1 labeling. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-84), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Immunocytochemistry-Immunofluorescence-NB100-615-img0014.jpg)
![Immunohistochemistry-Frozen: Caveolin-1 Antibody (7C8) [NB100-615] - Immunofluorescence of human adipose tissue. Primary antibody at 1:250. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Immunohistochemistry-Frozen-NB100-615-img0015.jpg)
![Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Flow-Cytometry-NB100-615-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Antibody at 1:200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane. Photo courtesy of Alberto Davalos, Yale School of Medicine.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Immunocytochemistry-Immunofluorescence-NB100-615-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Caveolin-1 [7C8] conjugated to Alexa Fluor 488 (NB100-615AF488) at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Immunocytochemistry-Immunofluorescence-NB100-615-img0013.jpg)
![Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Flow-Cytometry-NB100-615-img0008.jpg)
![Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Flow-Cytometry-NB100-615-img0011.jpg)
![Western Blot: Perilipin Antibody [NB110-40760] - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, probed with 2 ug/mL anti-perilipin antibody followed by anti-rabbit HRP conjugated secondary antibody and detected with chemiluminescence.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Western-Blot-NB110-40760-img0002.jpg "Western Blot: Perilipin Antibody [NB110-40760] - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, probed with 2 ug/mL anti-perilipin antibody followed by anti-rabbit HRP conjugated secondary antibody and detected with chemiluminescence.")
![Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NB110-40760-img0005.jpg)
![Flow Cytometry: Perilipin Antibody [NB110-40760] - An intracellular stain was performed on U2OS cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Flow-Cytometry-NB110-40760-img0008.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of fat tissue from mouse with Perilipin antibody at 1:200 dilution. The antibody generated an expected staining in the adipocytes towards periphery of the cells.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NB110-40760-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NB110-40760-img0006.jpg)
![Flow Cytometry: Perilipin Antibody [NB110-40760] - An intracellular stain was performed on MCF7 cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Flow-Cytometry-NB110-40760-img0007.jpg)
![Imaging Mass Cytometry: Perilipin Antibody [NB110-40760] - Human bone marrow analyzed by IMC. Perilipin antibody conjugated with 151Eu and used 1:50 on human bone marrow FFPE. Perilipin - red. DNA- white. IMC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Imaging-Mass-Cytometry-NB110-40760-img0009.jpg)
![Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Western-Blot-NB100-57086-img0006.jpg "Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NB100-57086] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57086-img0008.jpg)
![Immunohistochemistry-Paraffin: DGAT1 Antibody [NB100-57086] - Staining of paraffin embedded Human Colon. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunohistochemistry-Paraffin-NB100-57086-img0005.jpg)
![Flow Cytometry: DGAT1 Antibody [NB100-57086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Flow-Cytometry-NB100-57086-img0007.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0004.jpg "Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.")
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Immunofluorescence analysis of HepG2 cells using PLIN2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunocytochemistry-Immunofluorescence-NBP2-37300-img0005.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of bladder cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0008.jpg)
![Flow Cytometry: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of HepG2 cells using PLIN2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Flow-Cytometry-NBP2-37300-img0006.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against human PLIN2 (AA: 286-437) recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0002.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against HEK293 (1) and PLIN2 (AA: 286-437)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of rectum cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0007.jpg)
![ELISA: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-ELISA-NBP2-37300-img0001.jpg)
![Western Blot: Caveolin-1 Antibody [NB300-509] - Analysis of rat heart protein extract.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-Western-Blot-NB300-509-img0004.jpg "Western Blot: Caveolin-1 Antibody [NB300-509] - Analysis of rat heart protein extract.")
![Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Western-Blot-NBP2-59390-img0004.jpg "Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste")
![Immunocytochemistry/Immunofluorescence: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis of ATGL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Immunocytochemistry-Immunofluorescence-NBP2-59390-img0002.jpg)
![Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-AT18E6-Flow-Cytometry-NBP2-59390-img0006.jpg)
![Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis in A431 cell lysates (40ug), resolved by SDS-PAGE.](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Western-Blot-NBP2-59390-img0001.jpg)
![Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-AT18E6-Flow-Cytometry-NBP2-59390-img0005.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - ecombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Western-Blot-NBP2-53401-img0001.jpg "Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - ecombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).")
![Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - Flow Cytometry of human Perilipin-2/ADFP on PBMC. Green: Isotype Control; Red: Perilipin-2/ADFP Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Flow-Cytometry-NBP2-53401-img0004.jpg)
![Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - Flow Cytometry [NBP2-53401] - Flow Cytometry of human Adipophilin on PBMC. Green: Isotype Control; Red: Adipophilin Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Flow-Cytometry-NBP2-53401-img0003.jpg)
![Protein Array: Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-53401] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Perilipin-2/ADFP Mouse Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Protein-Array-NBP2-53401-img0005.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-54369] - recombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Azide-and-BSA-Free-Western-Blot-NBP2-54369-img0002.jpg "Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-54369] - recombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).")
![Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Flow Cytometry of human Perilipin-2/ADFP on PBMC. Green: Isotype Control; Red: Perilipin-2/ADFP Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54369-img0003.jpg)
![Protein Array: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Perilipin-2/ADFP Mouse Monoclonal Antibody (ADFP/1365).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1365-Azide-and-BSA-Free-Protein-Array-NBP2-54369-img0004.jpg)
![Western Blot: DGAT1 Antibody (SR1829) [NBP3-22666] - Western blot analysis using NBP3-22666 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22666_rabbit-dgat1-mab-sr1829-57202316494249.jpg "Western Blot: DGAT1 Antibody (SR1829) [NBP3-22666] - Western blot analysis using NBP3-22666 in HeLa cell lysate.")
![Flow Cytometry: Abhd5 Antibody (JE60-62) [NBP3-31989] - Flow cytometric analysis of NIH/3T3 cells labeling Abhd5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31989, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-31989_rabbit-abhd5-mab-je60-62-29202416453253.jpg "Flow Cytometry: Abhd5 Antibody (JE60-62) [NBP3-31989] - Flow cytometric analysis of NIH/3T3 cells labeling Abhd5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31989, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
![Western Blot: Abhd5 Antibody (JE60-62) [NBP3-31989] - Western blot analysis of Abhd5 on different lysates with Rabbit anti-Abhd5 antibody (NBP3-31989) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 ug/Lane) Lane 2: Mouse pancreas tissue lysate (40 ug/Lane) Lane 3: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 39 kDa Observed band size: 43 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31989) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31989_rabbit-abhd5-mab-je60-62-3920248453521.jpg)
![Western Blot: Caveolin-1 Antibody (2A7) [NBP3-26695] - Positive Western Blot detected in: THP-1 whole cell lysate. All lanes: Caveolin-1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 20 kDa Observed band size: 20 kDa](http://images.novusbio.com/fullsize/nbp3-26695_rabbit-caveolin-1-mab-2a7-28220241140941.jpg "Western Blot: Caveolin-1 Antibody (2A7) [NBP3-26695] - Positive Western Blot detected in: THP-1 whole cell lysate. All lanes: Caveolin-1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 20 kDa Observed band size: 20 kDa")
![Flow Cytometry: Caveolin-1 Antibody (2A7) [NBP3-26695] - Overlay Peak curve showing Hela cells stained with Caveolin-1 Antibody (2A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26695_rabbit-caveolin-1-mab-2a7-282202414312730.jpg)
![Western Blot: Abhd5 Antibody (SR1756) [NBP3-21931] - Western blot analysis using NBP3-21931 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21931_rabbit-abhd5-mab-sr1756-57202317215112.jpg "Western Blot: Abhd5 Antibody (SR1756) [NBP3-21931] - Western blot analysis using NBP3-21931 in HepG2 cell lysate.")