Hu,
Mu,
Rt,
Bv,
Ca,
Ch,
Pm,
Rb,
Re,
ZeSpecies Glossary
Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb, Re, Ze
WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-reported, Dual ISH-IHC, Single-Cell Western
Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-reported, Dual ISH-IHC, Single-Cell Western
Host: Mouse Monoclonal
Alt Catalog# NBO-135A
Sample Size Available
Sizes: Sample Size Available
![Dual RNAscope ISH-IHC: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - FFPE tissue sections of human metastatic tonsil were probed for Pan Cytokeratin mRNA (ACD RNAScope probe, catalog # 310221; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for IHC using mouse monoclonal antibody (Novus catalog # NBP2-29429) at 0.3 ug/mL for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Novus Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Dual-RNAscope-ISH-IHC-NBP2-29429-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - FFPE tissue sections of human metastatic tonsil were probed for Pan Cytokeratin mRNA (ACD RNAScope probe, catalog # 310221; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for IHC using mouse monoclonal antibody (Novus catalog # NBP2-29429) at 0.3 ug/mL for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Novus Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - MCF-7 cells stained with Biotin conjugated version of pan Cytokeratin antibody and SAv-A488. A) SAv-A488 only at a dilution of 1:500, B) pan CK at a dilution of 1:200, C) pan CK at a dilution of 1:400, D) pan CK at a dilution of 1:800. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Immunocytochemistry-Immunofluorescence-NBP2-29429-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Pan Cytokeratin was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 1.7 ug/mL of mouse anti-pan Cytokeratin monoclonal antibody (NBP2-29429, Novus Biologicals) for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer (Novus catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Immunohistochemistry-Paraffin-NBP2-29429-img0009.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Flow-(Intracellular)-NBP2-29429-img0014.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Immunohistochemistry-Paraffin-NBP2-29429-img0008.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Human Pan-Cytokeratins on HeLa Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Pan-Cytokeratin Monoclonal Antibody (AE-1/AE-3)](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Flow-Cytometry-NBP2-29429-img0004.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Pan-Cytokeratin Monoclonal Antibody (AE-1/AE-3).](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Flow-Cytometry-NBP2-29429-img0005.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Using the PE direct conjugate, an intracellular stain was performed on HT-29 cells with pan Cytokeratin (AE1 + AE3) antibody NBP2-33200PE (blue) and a matched isotype control NBP1-97005PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Flow-Cytometry-NBP2-29429-img0011.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image from the Alexa Fluor 647 version of this antibody.](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Flow-(Intracellular)-NBP2-29429-img0012.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Flow-(Intracellular)-NBP2-29429-img0013.jpg)
![CyTOF-reported: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Human fibroblasts. Blue: fibroblasts. Red: fibroblast + cytokeratin transfection. CyTOF image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-CyTOF-reported-NBP2-29429-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with rRNA Antibody [Y10b] conjugated to Biotin (NB100-662B) at 5ug/ml overnight at 4C and detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Immunocytochemistry-Immunofluorescence-NB100-662-img0015.jpg "Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with rRNA Antibody [Y10b] conjugated to Biotin (NB100-662B) at 5ug/ml overnight at 4C and detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow Cytometry: rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on HeLa cells with rRNA Antibody [Y10b] NB100-662B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Flow-Cytometry-NB100-662-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - rRNA antibody was tested in Ntera-2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Immunocytochemistry-Immunofluorescence-NB100-662-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - Staining of rRNA in vero cells.](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Immunocytochemistry-Immunofluorescence-NB100-662-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-rRNA (Y10b) conjugated to DyLight 488 [NB100-662G] at 5ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Immunocytochemistry-Immunofluorescence-NB100-662-img0009.jpg)
![Immunocytochemistry: rRNA Antibody (Y10b) [NB100-662] - Immunohistochemical staining of mouse neuronal progenitor culture using rRNA antibody (green). Image from verified customer review.](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Immunocytochemistry-NB100-662-img0005.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA antibody (Y10b) NB100-662 (blue) and an isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Flow-(Intracellular)-NB100-662-img0006.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis of Alexa Fluor (R) 647 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA (Y10b) antibody NB100-662AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1%](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Flow-(Intracellular)-NB100-662-img0007.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on RAW 246.7 cells with rRNA Antibody (Y10b) NB100-662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Flow-(Intracellular)-NB100-662-img0008.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on A549 cells with NB100-662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Flow-(Intracellular)-NB100-662-img0011.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on SH-SY5Y cells with rRNA (Y10b) antibody NB100-662AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-Flow-(Intracellular)-NB100-662-img0012.jpg)
![CyTOF-reported: rRNA Antibody (Y10b) [NB100-662] - Human bone marrow after RBC lysis, stained with anti-CD16, fixed, permeabilized with methanol, stained with anti-lactoferrin 1C6 NB120-10109 and anti-rRNA Y10b NB100-662, and gated on myeloid cells per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/rRNA-Antibody-(Y10b)-CyTOF-reported-NB100-662-img0014.jpg)
