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![Western Blot: WEHI-3 Whole Cell Lysate [NBP2-13176] - Western blot analysis using WEHI-3 whole cell lysate [NBP2-13176] (A) and HUVEC cell lysate (B). Primary antibody Beta actin (NB600-503) was used.](http://images.novusbio.com/fullsize/WEHI-3-Whole-Cell-Lysate-Western-Blot-NBP2-13176-img0004.jpg "Western Blot: WEHI-3 Whole Cell Lysate [NBP2-13176] - Western blot analysis using WEHI-3 whole cell lysate [NBP2-13176] (A) and HUVEC cell lysate (B). Primary antibody Beta actin (NB600-503) was used.")
![Western Blot: U-87 MG Whole Cell Lysate [NBP3-06996] - Western Blot Results of Rabbit Anti-SQSTM1/p62 Antibody using U-87 MG Whole Cell lysate. Western Blot of Rabbit Anti-SQSTM1/p62 Antibody.Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: U-87 MG whole cell lysate. Load: 35ug lysate per lane. Primary Antibody: Anti-SQSTM1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 60 mins at RT. Block: 5% BLOTTO/TBST. Predicted MW: ~48 kDa. Observed MW: ~45, 60kDa. Protein undergoes acetylation, Isopeptide bond, Phosphoprotein, and Ubl conjugation.](http://images.novusbio.com/fullsize/U-87-MG-Whole-Cell-Lysate-Western-Blot-NBP3-06996-img0002.jpg "Western Blot: U-87 MG Whole Cell Lysate [NBP3-06996] - Western Blot Results of Rabbit Anti-SQSTM1/p62 Antibody using U-87 MG Whole Cell lysate. Western Blot of Rabbit Anti-SQSTM1/p62 Antibody.Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: U-87 MG whole cell lysate. Load: 35ug lysate per lane. Primary Antibody: Anti-SQSTM1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 60 mins at RT. Block: 5% BLOTTO/TBST. Predicted MW: ~48 kDa. Observed MW: ~45, 60kDa. Protein undergoes acetylation, Isopeptide bond, Phosphoprotein, and Ubl conjugation.")
![SDS-Page: U-87 MG Whole Cell Lysate [NBP3-06996] - SDS-PAGE Results of U-87 MG Whole Cell Lysate. Lane 1: U-87 MG Whole Cell lysate(10ug) Reduced. Lane 2: Opal Prestained Molecular Weight. Lane 3: U-87 MG Whole Cell lysate(10ug) Non-Reduced. Results show wide range of molecular weight bands with no signs of degradation.](http://images.novusbio.com/fullsize/U-87-MG-Whole-Cell-Lysate-SDS-Page-NBP3-06996-img0001.jpg)
![Western Blot: CHO K1 Whole Cell Lysate [NB800-PC13] - Detection of alpha tubulin in lane 1. CHO/K1 Whole Cell Lysate (10 g) was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, primary antibody was used at 1:1000 overnight at 4C. Anti-Rabbit IgG (H&L) (GOAT) antibody IRDye800CW secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting and imaged on the LiCor Odyssey imaging system. Arrow indicates correct 50 kDa molecular weight position expected for alpha tubulin.](http://images.novusbio.com/fullsize/CHO-K1-Whole-Cell-Lysate-Western-Blot-NB800-PC13-img0007.jpg "Western Blot: CHO K1 Whole Cell Lysate [NB800-PC13] - Detection of alpha tubulin in lane 1. CHO/K1 Whole Cell Lysate (10 g) was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, primary antibody was used at 1:1000 overnight at 4C. Anti-Rabbit IgG (H&L) (GOAT) antibody IRDye800CW secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting and imaged on the LiCor Odyssey imaging system. Arrow indicates correct 50 kDa molecular weight position expected for alpha tubulin.")
![Western Blot: CHO K1 Whole Cell Lysate [NB800-PC13] - Coomassie stained SDS-PAGE of 35 ul of CHO Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under reducing conditions (lane 2). Molecular weight standards are shown in lane 1.](http://images.novusbio.com/fullsize/CHO-K1-Whole-Cell-Lysate-Western-Blot-NB800-PC13-img0004.jpg)
![SDS-Page: MDA-MB-231 Whole Cell Lysate [NBP3-18060] - SDS PAGE Results of MDA-MB-231 Whole Cell Lysate. Lane 1: MDA-MB-231 Whole Cell Lysate Reduced [10ug]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: MDA-MB-231 Whole Cell Lysate Non-Reduced [10ug]. 4-20% Gel, Coomassie Stained.](http://images.novusbio.com/fullsize/MDA-MB-231-Whole-Cell-Lysate-SDS-Page-NBP3-18060-img0001.jpg "SDS-Page: MDA-MB-231 Whole Cell Lysate [NBP3-18060] - SDS PAGE Results of MDA-MB-231 Whole Cell Lysate. Lane 1: MDA-MB-231 Whole Cell Lysate Reduced [10ug]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: MDA-MB-231 Whole Cell Lysate Non-Reduced [10ug]. 4-20% Gel, Coomassie Stained.")
![Western Blot: U-251 Whole Cell Lysate [NBP3-06998] - Western Blot results of Rabbit Anti-Beclin 1 Antibody using U-251 Whole Cell lysate.Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: U251-MG whole cell lysate (35ug) [+]. Primary Antibody: Anti-Beclin1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 60min at RT. Block: 5% BLOTTO/TBST.Expected MW: ~51kDa. Observed MW: ~53kDa.](http://images.novusbio.com/fullsize/U-251-Whole-Cell-Lysate-Western-Blot-NBP3-06998-img0002.jpg "Western Blot: U-251 Whole Cell Lysate [NBP3-06998] - Western Blot results of Rabbit Anti-Beclin 1 Antibody using U-251 Whole Cell lysate.Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: U251-MG whole cell lysate (35ug) [+]. Primary Antibody: Anti-Beclin1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 60min at RT. Block: 5% BLOTTO/TBST.Expected MW: ~51kDa. Observed MW: ~53kDa.")
![SDS-Page: U-251 Whole Cell Lysate [NBP3-06998] - SDS-PAGE Results of U-251 Whole Cell Lysate. Lane 1: U-251 Whole Cell lysate(10ug) Reduced. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: U-251 Whole Cell lysate(10ug) Non-Reduced. Results show wide range of molecular weight bands with no sign of degradation.](http://images.novusbio.com/fullsize/U-251-Whole-Cell-Lysate-SDS-Page-NBP3-06998-img0001.jpg)
![Western Blot: Raji Whole Cell Lysate [NB820-59466] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.](http://images.novusbio.com/fullsize/Raji-Whole-Cell-Lysate-Western-Blot-NB820-59466-img0002.jpg "Western Blot: Raji Whole Cell Lysate [NB820-59466] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.")
![SDS-Page: Raji Whole Cell Lysate [NB820-59466] - Protein lysate shows consistent pattern.](http://images.novusbio.com/fullsize/Raji-Whole-Cell-Lysate-SDS-Page-NB820-59466-img0001.jpg)
![SDS-Page: Hep2 Whole Cell Lysate [NB800-PC7] - Coomassie stained SDS-PAGE of 20 ul of Human Derived (Ready-to-Use) separated in a 4-20% gradient gel under reducing conditions (lane 2). Molecular weight standards are shown in lane 1.](http://images.novusbio.com/fullsize/Hep2-Whole-Cell-Lysate-Western-Blot-NB800-PC7-img0003.jpg "SDS-Page: Hep2 Whole Cell Lysate [NB800-PC7] - Coomassie stained SDS-PAGE of 20 ul of Human Derived (Ready-to-Use) separated in a 4-20% gradient gel under reducing conditions (lane 2). Molecular weight standards are shown in lane 1.")
![Western Blot: MEF Whole Cell Lysate [NB800-PC10] - Coomassie stain of 0.02 ml of NB 800-PC10 separated on a 4-20% gradient gel under non-reducing conditions.](http://images.novusbio.com/fullsize/MEF-Whole-Cell-Lysate-Western-Blot-NB800-PC10-img0002.jpg "Western Blot: MEF Whole Cell Lysate [NB800-PC10] - Coomassie stain of 0.02 ml of NB 800-PC10 separated on a 4-20% gradient gel under non-reducing conditions.")
![SDS-Page: U-138 MG Whole Cell Lysate [NBP3-06999] - SDS-PAGE Results of U-138 MG Whole Cell Lysate. Lane 1: U-138 MG Whole Cell lysate(10ug) Reduced. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: U-138 MG Whole Cell lysate(10ug) Non-Reduced. Results show wide range of molecular weight bands and no sign of degradation.](http://images.novusbio.com/fullsize/U-138-MG-Whole-Cell-Lysate-SDS-Page-NBP3-06999-img0001.jpg "SDS-Page: U-138 MG Whole Cell Lysate [NBP3-06999] - SDS-PAGE Results of U-138 MG Whole Cell Lysate. Lane 1: U-138 MG Whole Cell lysate(10ug) Reduced. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: U-138 MG Whole Cell lysate(10ug) Non-Reduced. Results show wide range of molecular weight bands and no sign of degradation.")
![SDS-Page: Daudi Whole Cell Lysate [NBP3-05285] - Lane 1: Daudi Whole Cell Lysate (10ug) Reduced. Lane 2: Opal Prestained Molecular Weight Markers. Lane 3: Daudi Whole Cell Lysate (10ug) Non-Reduced. Results show wide range of molecular weight bands with no signs of degradation.](http://images.novusbio.com/fullsize/Daudi-Whole-Cell-Lysate-SDS-Page-NBP3-05285-img0002.jpg "SDS-Page: Daudi Whole Cell Lysate [NBP3-05285] - Lane 1: Daudi Whole Cell Lysate (10ug) Reduced. Lane 2: Opal Prestained Molecular Weight Markers. Lane 3: Daudi Whole Cell Lysate (10ug) Non-Reduced. Results show wide range of molecular weight bands with no signs of degradation.")
![SDS-Page: PC-3 Whole Cell Lysate [NBP3-06995] - SDS-PAGE of PC-3 Whole Cell Lysate. Lane 1: PC-3 Whole Cell lysate(10ug) Reduced. Lane 2: Opal Prestained Molecular weight marker. Lane 3: PC-3 Whole Cell lysate(10ug) Non-Reduced. Results show wide range of molecular weight bands with no signs of degradation.](http://images.novusbio.com/fullsize/PC-3-Whole-Cell-Lysate-SDS-Page-NBP3-06995-img0001.jpg "SDS-Page: PC-3 Whole Cell Lysate [NBP3-06995] - SDS-PAGE of PC-3 Whole Cell Lysate. Lane 1: PC-3 Whole Cell lysate(10ug) Reduced. Lane 2: Opal Prestained Molecular weight marker. Lane 3: PC-3 Whole Cell lysate(10ug) Non-Reduced. Results show wide range of molecular weight bands with no signs of degradation.")