| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73200 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88558-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43954 | |||
| BSA Free | NBP2-34495-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71256 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14835SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1925SS | |||
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0018.jpg "Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: p53 Antibody (5E2) [NBP2-00723] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunocytochemistry-Immunofluorescence-NBP2-00723-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0016.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-p53 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0012.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0014.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0015.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - Analysis of Hela cells, using anti-p53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0011.jpg)
![Simple Western: p53 Antibody (5E2) [NBP2-00723] - Simple Western lane view shows a specific band for p53 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Simple-Western-NBP2-00723-img0010.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining in human placenta and pancreas tissues using anti-BNIP3L antibody. Corresponding BNIP3L RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP1-88558-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining in human placenta and pancreas tissues using anti-BNIP3L antibody. Corresponding BNIP3L RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BNIP3L Antibody [NBP1-88558] - Staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88558-img0006.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP1-88558-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP1-88558] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP1-88558-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416113531.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416114791.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416114754.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-310202416121953.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody [NBP1-88558] - The role of PA in BNIP3 silenced UCB-hMSC survival in the mouse skin wound healing model. (A) Mouse skin wound surgery with UCB-hMSC transplantation was performed as described in Section 2. Representative gross wound images were acquired at post injection days 0, 4, 8, 12. Skin wound sizes at day 8 were compared with wound size at day 0. n = 5. (B) Tissue slide samples were stained with hematoxylin & eosin. Low & high magnified histological gross images are shown in the left & right panels, respectively. Scale bars, 260 µm (magnification, ×40) & 100 µm (magnification, ×100). n = 5. (C) Representative images of blood vessels in skin wounds on day 12 (top panel). Vessel density was analyzed by using ImageJ program (bottom panel). n = 5. (D-F) Histological tissue samples were immuno-stained with CD31, alpha -SMA, & HNA-specific antibodies & PI for counterstaining. alpha -SMA & HNA-positive cells were visualized by confocal microscopy. The number of CD31 & alpha -SMA-positive cells in high power field (HPF), & the percentage of HNA-positive cells in total cells were analyzed by using Metamorph software. Scale bars, 100 µm (magnification, ×100). n = 5. Data are presented as a mean ± S.E.M. $p < 0.05 versus vehicle group, *p < 0.05 versus UCB-hMSC group given NT siRNA, #p < 0.05 versus UCB-hMSC group given NT siRNA with hypoxia pretreatment, @p < 0.05 versus UCB-hMSC group given BNIP3 siRNA with hypoxia pretreatment. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88558_rabbit-polyclonal-bnip3l-antibody-31020241613647.jpg)
![Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0004.jpg "Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ATR Antibody [NB100-323] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATR antibody NB100-323 lot 3 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ATR was also immunoprecipitated by a previous lot (lot 2) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0002.jpg)
![Western Blot: ATR Antibody [NB100-323] - H1299 (human lung cancer cells) and HCC 44 (human liver cancer cells). Antibody at 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0003.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091472.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242010445.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091496.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-910202420134015.jpg)
![MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 ug Jurkat cell whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody (NBP2-17247)7.5 % SDS-PAGEThe immunoprecipitated MDM2 protein was detected by MDM2 antibody (NBP2-17247) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-immunoprecipitation-261120251826130.jpg)
![Western Blot: ATM Antibody [NB100-678] - Samples: A) Whole cell lysate (50 ug) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit ATM antibody [NB100-678] used at the indicated concentrations for WB and at 0.5 ug/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~370 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0003.jpg "Western Blot: ATM Antibody [NB100-678] - Samples: A) Whole cell lysate (50 ug) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit ATM antibody [NB100-678] used at the indicated concentrations for WB and at 0.5 ug/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~370 kDa. Theoretical molecular weight 351 kDa.")
![Western Blot: ATM Antibody [NB100-678] - MDA-MB-231 and MCF-7 whole cell lysates were loaded with 50 ug/lane. 8% SDS-PAGE. ATM Antibody (NB100-678) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0009.jpg)
![Immunohistochemistry: ATM Antibody [NB100-678] - FFPE section of human prostate-nodular hypertrophy. Affinity purified rabbit [NB100-678] used at a dilution of 1:1000 ( 1ug/mL). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/ATM-Antibody-Immunohistochemistry-NB100-678-img0007.jpg)
![Western Blot: ATM Antibody [NB100-678] - Analysis in whole cell lysates from human lung cancer lines with [NB100-678]. Theoretical molecular weight 351 kDa. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-678-img0008.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-2611202518354313.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-P53 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U2OS cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0014.jpg)
![Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining of human cell line A431 showing cell cycle dependent nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in MCF7 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U251 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells, but not in normal mucosa.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human lung cancer (squamous cell carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0018.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skin shows moderate to strong nuclear positivity in a subset of epidermal cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0019.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0020.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Simple Western lane view shows a specific band for P53 in 0.2 mg/ml of Hek293T lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0009.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Electropherogram image(s) of corresponding Simple Western lane view. p53 antibody was used at 1:50 dilution on Hek293T lysate(s).](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0011.jpg)
![Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Western-Blot-NBP1-48284-img0001.jpg "Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] Staining of COS7 cells transiently transfected by pCMV6-ENTRY JNK1.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48284-img0008.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human pancreas tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] - Immunofluorescent staining of HeLa cells using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48284-img0002.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0003.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human Kidney tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human liver tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0005.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human Ovary tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0006.jpg)
![Western Blot: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in human testes lysate.](http://images.novusbio.com/fullsize/LKB1-Antibody-Western-Blot-NBP2-14835-img0001.jpg "Western Blot: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in human testes lysate.")
![Immunocytochemistry/Immunofluorescence: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/LKB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14835-img0003.jpg)
![Immunohistochemistry-Paraffin: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in mouse stomach using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/LKB1-Antibody-Immunohistochemistry-Paraffin-NBP2-14835-img0002.jpg)
![Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.](http://images.novusbio.com/fullsize/JNK1-Antibody-Western-Blot-NB100-1925-img0004.jpg "Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.")
![Immunocytochemistry/Immunofluorescence: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249432824.jpg "Immunocytochemistry/Immunofluorescence: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249415956.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249413336.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-212024940822.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249432899.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249355569.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249374974.jpg)