| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15131-25ul | |||
| Azide and BSA Free | NBP3-44013 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03444-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24771SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04982-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43874 | |||
![Immunohistochemistry-Paraffin: Furin Antibody [NBP2-24687] - analysis of Furin in formalin-fixed, paraffin-embedded human kidney tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/Furin-Antibody-Immunohistochemistry-Paraffin-NBP2-24687-img0001.jpg "Immunohistochemistry-Paraffin: Furin Antibody [NBP2-24687] - analysis of Furin in formalin-fixed, paraffin-embedded human kidney tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.")
![Western Blot: Nodal Antibody [NBP1-71686] - Analysis of NODAL in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/Nodal-Antibody-Western-Blot-NBP1-71686-img0006.jpg "Western Blot: Nodal Antibody [NBP1-71686] - Analysis of NODAL in HeLa whole cell extracts.")
![Immunocytochemistry/Immunofluorescence: Nodal Antibody [NBP1-71686] - ICC staining of NODAL in HeLa cells.](http://images.novusbio.com/fullsize/Nodal-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71686-img0005.jpg)
![Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NB600-879-img0005.jpg "Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.")
![Immunohistochemistry-Paraffin: Notch-2 Antibody [NB600-879] - Diluted 1:500 to detect Notch-2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NB600-879-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (CL12191) [NBP3-15131] -Staining of RH-30 cells using the Anti-GLI1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/antibody/nbp3-15131_mouse-gli-1-mab-cl12191-immunocytochemistry-immunofluorescence-41020238465..jpg "Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (CL12191) [NBP3-15131] -Staining of RH-30 cells using the Anti-GLI1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![Western Blot: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Lane A: Marker; Lane B HepG2 Cell Lysate. Antibody titration 1.0ug/ml. Gel concentration 12%](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Western-Blot-NBP1-69270-img0005.jpg "Western Blot: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Lane A: Marker; Lane B HepG2 Cell Lysate. Antibody titration 1.0ug/ml. Gel concentration 12%")
![Immunohistochemistry: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Chicken embryos Primary Antibody: 1 : 1000 (NBP1-69270) anti -shh Secondary Antibody: 1 : 500 goat anti-rabbit HRP conjugated.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Immunohistochemistry-NBP1-69270-img0009.jpg)
![Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Human Intestine cells with positive label: Epithelial cells of intestinal villus (indicated with arrows). Concentration 4.0-8.0 ug/ml](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Immunohistochemistry-Paraffin-NBP1-69270-img0002.jpg)
![Immunohistochemistry: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Human glioma cells. Primary Antibody Dilution :1:200 Secondary Antibody :Anti-rabbit-GFP. Secondary Antibody Dilution :1:500. Color/Signal Descriptions :1. SHH: Green 2. DAPI: Blue 3. Merge. Gene Name :SHH](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Immunohistochemistry-NBP1-69270-img0007.jpg)
![Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of Rat lung, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Western-Blot-NBP3-15671-img0004.jpg "Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of Rat lung, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (2E3D8) [NBP3-15671] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad2 Rabbit mAb (NBP3-15671) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Immunocytochemistry-Immunofluorescence-NBP3-15671-img0002.jpg)
![Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of various cell lines, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Western-Blot-NBP3-15671-img0003.jpg)
![Immunoprecipitation: Smad2 Antibody (2E3D8) [NBP3-15671] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Smad2 antibody (NBP3-15671). Western blot was performed from the immunoprecipitate using Smad2 antibody (NBP3-15671) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Immunoprecipitation-NBP3-15671-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Smad2 Antibody (2E3D8) [Smad2] - Western blot analysis of lysates from wild type (WT) and Smad2 knockdown (KD) HeLa cells using [KD Validated] Smad2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15671_rabbit-smad2-mab-2e3d8-52202517304995.jpg)
![Immunocytochemistry/ Immunofluorescence: Smad2 Antibody (2E3D8) [Smad2] - Confocal imaging of HeLa cells using [KD Validated] Smad2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15671_rabbit-smad2-mab-2e3d8-522025173455.jpg)
![Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1: 293T cell lysateLane 2: Wnt3a Transfected 293T cell lysate](http://images.novusbio.com/fullsize/Wnt-3a-Antibody-3A6-Western-Blot-NBP1-19050-img0007.jpg "Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1: 293T cell lysateLane 2: Wnt3a Transfected 293T cell lysate")
![Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) [NBP1-19050] - Human breast cancer tissue were incubated with anti-human Wn-t3a for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).](http://images.novusbio.com/fullsize/Wnt-3a-Antibody-3A6-Immunohistochemistry-Paraffin-NBP1-19050-img0004.jpg)
![Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Wnt-3a-Antibody-3A6-Western-Blot-NBP1-19050-img0006.jpg)
![Western Blot: JMJD6/PSR Antibody [NBP3-03444] - Western blot analysis of extracts of 293T cells, using JMJD6/PSR antibody (NBP3-03444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/JMJD6-PSR Antibody-Western Blot-NBP3-03444-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: JMJD6/PSR Antibody [NBP3-03444] - Western blot analysis of extracts of 293T cells, using JMJD6/PSR antibody (NBP3-03444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: JMJD6/PSR Antibody [NBP3-03444] - Immunohistochemistry of paraffin-embedded mouse spinal cord using JMJD6/PSR Rabbit pAb (NBP3-03444) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/JMJD6-PSR-Antibody-Immunohistochemistry-Paraffin-NBP3-03444-img0003.jpg)
![Immunohistochemistry-Paraffin: JMJD6/PSR Antibody [NBP3-03444] - Immunohistochemistry of paraffin-embedded rat brain using JMJD6/PSR Rabbit pAb (NBP3-03444) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/JMJD6-PSR-Antibody-Immunohistochemistry-Paraffin-NBP3-03444-img0001.jpg)
![Immunohistochemistry-Paraffin: JMJD6/PSR Antibody [NBP3-03444] - Immunohistochemistry of paraffin-embedded human lung using JMJD6/PSR Rabbit pAb (NBP3-03444) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/JMJD6-PSR-Antibody-Immunohistochemistry-Paraffin-NBP3-03444-img0002.jpg)
![Western Blot: Notch-1 Antibody [NBP2-24771] - Analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5 mM EDTA for 15 minutes, using this antibody at 3 ug/mL. The EDTA treatment increases the detected level of Notch1-cv.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP2-24771-img0002.jpg "Western Blot: Notch-1 Antibody [NBP2-24771] - Analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5 mM EDTA for 15 minutes, using this antibody at 3 ug/mL. The EDTA treatment increases the detected level of Notch1-cv.")
![Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extracts from Hela cells untreated or treeated with PMAA using Smadd2 (Phospho-Thhr220) Antibody .](http://images.novusbio.com/fullsize/Smad2-[p-Thr220]-Antibody-Western-Blot-NBP1-04982-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extracts from Hela cells untreated or treeated with PMAA using Smadd2 (Phospho-Thhr220) Antibody .")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Smad2 [p Thr220] Antibody [NBP1-04982] - Immunohistochemical analysis of Smad2 staining in human brain formalin fixed paraffin embedded tissue section.](http://images.novusbio.com/fullsize/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-234202415232511.jpg)
![Immunocytochemistry/ Immunofluorescence: Smad2 [p Thr220] Antibody [NBP1-04982] - Immunofluorescent analysis of Smad2 staining in HeLa cells.](http://images.novusbio.com/fullsize/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-234202414462213.jpg)
![Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Western blot analysis of Smad2 [p Thr220] expression in rat heart (A) whole cell lysates.](http://images.novusbio.com/fullsize/nbp1-04982_rabbit-polyclonal-smad2-p-thr220-antibody-23420241452107.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)](http://images.novusbio.com/fullsize/nbp1-88030_rabbit-polyclonal-cerberus-1-antibody-6520251619368.jpg)
![Western Blot: Smad4 Antibody (CL9774) [NBP3-05507] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Western-Blot-NBP3-05507-img0006.jpg "Western Blot: Smad4 Antibody (CL9774) [NBP3-05507] - Analysis in human cell line SCLC-21H.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human placenta shows moderate nuclear and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human prostate shows moderate nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human stomach cancer shows strong cytoplasmic and nuclear positivity.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0004.jpg)