| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76792-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73030 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86337-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38325-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38774-25ul | |||
![Western Blot: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.](http://images.novusbio.com/fullsize/Wnt-1-Antibody-10C8-Western-Blot-NBP1-51575-img0008.jpg "Western Blot: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Wnt-1 Antibody (10C8) [NBP1-51575] - Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Wnt-1-Antibody-10C8-Immunocytochemistry-Immunofluorescence-NBP1-51575-img0006.jpg)
![Immunohistochemistry-Paraffin: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis of human LAdrenal tissues using WNT1 mouse mAb.](http://images.novusbio.com/fullsize/Wnt-1-Antibody-10C8-Immunohistochemistry-Paraffin-NBP1-51575-img0007.jpg)
![Flow Cytometry: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis of Hela cells using WNT1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Wnt-1-Antibody-10C8-Flow-Cytometry-NBP1-51575-img0009.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249432892.jpg "Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249422641.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - Wild-type (WT) and CREBBP knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-212024942543.jpg)
![Western Blot: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249393587.jpg)
![Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249432876.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-western-blot-261120251830385.jpg)
![Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25753_rabbit-wnt-3a-mab-hl1911-azide-and-bsa-free-2120249425414.jpg "Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25753_rabbit-wnt-3a-mab-hl1911-azide-and-bsa-free-2120249393516.jpg)
![Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25753_rabbit-wnt-3a-mab-hl1911-azide-and-bsa-free-212024941499.jpg)
![ICC/IF Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: Wnt3a stained by Wnt3a antibody [HL1911] diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10μm.](http://images.novusbio.com/fullsize/nbp3-25753_rabbit-wnt-3a-mab-hl1911-azide-and-bsa-free-immunocytochemistry-immunofluorescence-272202584122..png)
![Immunohistochemistry-Paraffin Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Rat tissues.Wnt3a stained by Wnt3a antibody [HL1911] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25753_rabbit-wnt-3a-mab-hl1911-azide-and-bsa-free-immunohistochemistry-paraffin-27220258435..png)
![Immunohistochemistry-Paraffin Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Human prostate cancer.Wnt3a stained by Wnt3a antibody [HL1911] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-25753_rabbit-wnt-3a-mab-hl1911-azide-and-bsa-free-immunohistochemistry-paraffin-272202584453..png)
![Western Blot: Presenilin-1 Antibody [NBP1-76792] - Human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-Western-Blot-NBP1-76792-img0002.jpg "Western Blot: Presenilin-1 Antibody [NBP1-76792] - Human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody [NBP1-76792] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) Presenilin-1. Overlay images indicate the possible co-localization of limited co-localization of VDR/ Presenilin-1 on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76792-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Presenilin-1 Antibody - BSA Free [NBP1-76792] - Immunofluorescence of Presenilin-1 in Human Brain cells with Presenilin-1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76792_rabbit-polyclonal-presenilin-1-antibody-2711202419201530.jpg)
![Immunohistochemistry: Presenilin-1 Antibody - BSA Free [NBP1-76792] - Immunohistochemistry of Presenilin-1 in human brain tissue with Presenilin-1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76792_rabbit-polyclonal-presenilin-1-antibody-271120242092064.jpg)
![Western Blot: Noggin Antibody (OTI1H8) [NBP1-47881] - Noggin Antibody (1H8) [NBP1-47881] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Noggin monoclonal antibody.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Western-Blot-NBP1-47881-img0012.jpg "Western Blot: Noggin Antibody (OTI1H8) [NBP1-47881] - Noggin Antibody (1H8) [NBP1-47881] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Noggin monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Noggin Antibody (OTI1H8) [NBP1-47881] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Noggin.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Immunocytochemistry-NBP1-47881-img0007.jpg)
![Immunohistochemistry-Paraffin: Noggin Antibody (1H8) [NBP1-47881] - Staining of paraffin-embedded Human Ovary tissue using anti-Noggin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Immunohistochemistry-Paraffin-NBP1-47881-img0009.jpg)
![Western Blot: Noggin Antibody (1H8) [NBP1-47881] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Noggin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Noggin.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Western-Blot-NBP1-47881-img0010.jpg)
![Western Blot: Noggin Antibody (1H8) [NBP1-47881] - Noggingin antibody (1H8) at 1:40000 + Recombinant human Noggingin.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Western-Blot-NBP1-47881-img0011.jpg)
![Immunohistochemistry-Paraffin: Noggin Antibody (1H8) [NBP1-47881] - Staining of paraffin-embedded Human lung tissue using anti-Noggin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Immunohistochemistry-Paraffin-NBP1-47881-img0008.jpg)
![Western Blot: GLI-1 Antibody (JF09-08) [NBP2-66867] - Western blot analysis of GLI-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: SH-SY5Y cell lysate](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Western-Blot-NBP2-66867-img0007.jpg "Western Blot: GLI-1 Antibody (JF09-08) [NBP2-66867] - Western blot analysis of GLI-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: SH-SY5Y cell lysate")
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867] - Staining Gli1 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Immunocytochemistry-Immunofluorescence-NBP2-66867-img0003.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody (JF09-08) [NBP2-66867] - Analysis of paraffin-embedded rat testis tissue using anti-Gli1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Immunohistochemistry-Paraffin-NBP2-66867-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867] - Staining Gli1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Immunocytochemistry-Immunofluorescence-NBP2-66867-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867] - Staining Gli1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Immunocytochemistry-Immunofluorescence-NBP2-66867-img0002.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody (JF09-08) [NBP2-66867] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-Gli1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Immunohistochemistry-Paraffin-NBP2-66867-img0005.jpg)
![Western Blot: CBP/KAT3A Antibody [NB100-382] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Western-Blot-NB100-382-img0008.jpg "Western Blot: CBP/KAT3A Antibody [NB100-382] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry: CBP/KAT3A Antibody [NB100-382] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBP used at a dilution of 1:500 (2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-NB100-382-img0007.jpg)
![Immunohistochemistry: CBP/KAT3A Antibody [NB100-382] - FFPE section of human prostate adenocarcinoma. Antibody used at a dilution of 1:250. Detection: DAB.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-NB100-382-img0003.jpg)
![Immunoprecipitation: CBP/KAT3A Antibody [NB100-382] - Samples: Nuclear (NE) or cytoplasmic (S100) extract (10 mg) from HeLa cells. Antibody: Affinity purified rabbit anti-CBP used at 20 ug/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is CBP.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunoprecipitation-NB100-382-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CBP/KAT3A Antibody [NB100-382] - Detection of human CBP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for IP at 6 ug/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody NB100-381. For blotting immunoprecipitated CBP, NB100-382 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunoprecipitation-NB100-382-img0009.jpg)
![Western Blot: Jagged 1 Antibody [NB600-1161] - Analysis of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at 134 kDa in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kDa.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Western-Blot-NB600-1161-img0004.jpg "Western Blot: Jagged 1 Antibody [NB600-1161] - Analysis of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at 134 kDa in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kDa.")
![Immunocytochemistry/Immunofluorescence: Jagged 1 Antibody [NB600-1161]](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1161-img0005.jpg)
![Immunohistochemistry: Jagged 1 Antibody [NB600-1161] - Human cervical cancer tissue (40X magnification) using NB600-1161 Protein A purified anti- Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-NB600-1161-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Jagged 1 Antibody [NB600-1161] - Human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue).](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1161-img0002.jpg)
![Immunohistochemistry-Paraffin: Jagged 2 Antibody [NBP1-86337] - Staining of human cervix, uterine shows moderate membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Jagged-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86337-img0008.jpg "Immunohistochemistry-Paraffin: Jagged 2 Antibody [NBP1-86337] - Staining of human cervix, uterine shows moderate membranous positivity in squamous epithelial cells.")
![Immunohistochemistry-Paraffin: Jagged 2 Antibody [NBP1-86337] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Jagged-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86337-img0006.jpg)
![Immunohistochemistry-Paraffin: Jagged 2 Antibody [NBP1-86337] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Jagged-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86337-img0007.jpg)
![Immunohistochemistry-Paraffin: Jagged 2 Antibody [NBP1-86337] - Staining of human skeletal muscle shows no positivity as expected.](http://images.novusbio.com/fullsize/Jagged-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86337-img0009.jpg)
![Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] - Non-transfected (-) and transfected (+) RMS13 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GLI-1-Antibody-HL247-Western-Blot-NBP3-13667-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] - Non-transfected (-) and transfected (+) RMS13 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GLI-1 Antibody (HL247) [NBP3-13667] - GLI-1 antibody [HL247] detects GLI-1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GLI-1 stained by GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GLI-1-Antibody-HL247-Immunohistochemistry-Paraffin-NBP3-13667-img0002.jpg)
![Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GLI-1-Antibody-HL247-Western-Blot-NBP3-13667-img0003.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody (HL247) [NBP3-13667] - GLI-1 antibody [HL27] detects GLI-1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.GLI-1 stained by GLI-1 antibody [HL27] (NBP3-13667) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13667_rabbit-gli-1-mab-hl247-9102024205649.jpg)
![RMS 13 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (NBP3-13667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13667_rabbit-gli-1-mab-hl247-western-blot-26112025197181.jpg)
![Gli1 antibody [HL247] detects Gli1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Gli1 stained by Gli1 antibody [HL247] (NBP3-13667) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13667_rabbit-gli-1-mab-hl247-immunocytochemistry-immunofluorescence-26112025193393.jpg)
![Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL27] (NBP3-13667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13667_rabbit-gli-1-mab-hl247-western-blot-2611202518185021.jpg)
![Western Blot: Nucleostemin Antibody [NBP2-38325] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NBP2-38325-img0003.jpg "Western Blot: Nucleostemin Antibody [NBP2-38325] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human skin.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38325-img0007.jpg)
![Immunohistochemistry: Nucleostemin Antibody [NBP2-38325] - Staining of human skin shows strong nucleolar positivity in epidermal cells.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NBP2-38325-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human kidney, liver, skin and testis using Anti-GNL3 antibody NBP2-38325 (A) shows similar protein distribution across tissues to independent antibody NBP2-38326 (B).](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38325-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human testis.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38325-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human liver.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38325-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human kidney.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38325-img0008.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Analysis in human endometrium and liver tissues. Corresponding CREBBP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Analysis in human endometrium and liver tissues. Corresponding CREBBP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody [NBP2-38774] - Staining of human cell line MCF7 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38774-img0001.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human liver shows very weak nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0002.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human endometrium shows strong nuclear positivity in glandular cells and stromal cells.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0003.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0004.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0005.jpg)