| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89989-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70509 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54540-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77044-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52424-0.025mg | |||
![Western Blot: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysateLane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Western-Blot-NBP2-67179-img0006.jpg "Western Blot: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysateLane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0005.jpg)
![Flow Cytometry: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of Hela cells with ADAM17 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Flow-Cytometry-NBP2-67179-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0003.jpg)
![Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Rat hippocampus tissue with Rabbit anti-ADAM17 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-paraffin-2272024112433..jpg)
![Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] -Mouse hippocampus tissue with Rabbit anti-ADAM17 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-paraffin-2272024112623..jpg)
![IHC-Frozen: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] -Analysis of frozen mouse cerebral cortex tissue labeling ADAM17 with Rabbit anti-ADAM17 antibody.The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-frozen-2272024112828..jpg)
![IHC-Frozen: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] -Analysis of frozen mouse hippocampus tissue labeling ADAM17 with Rabbit anti-ADAM17 antibody.The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-frozen-2272024112950..jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-89989] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89989-img0011.jpg "Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-89989] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining in human cervix, uterine and skeletal muscle tissues . Corresponding CTNNB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0025.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human cervix, uterine shows moderate membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0020.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human skeletal muscle shows weak positivity in myocytes.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0024.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0023.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human duodenum shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0021.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89989-img0022.jpg)
![Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Western-Blot-NB300-749-img0007.jpg "Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749] - Mouse fibroblasts.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunocytochemistry-Immunofluorescence-NB300-749-img0004.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0006.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0005.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP2-15281-img0005.jpg "Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.")
![Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP2-15281] - Human breast carcinoma dilution: 1:250. ADAM17 antibody [C2C3], C-term dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Immunohistochemistry-Paraffin-NBP2-15281-img0006.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - 30 ug K562 whole cell lysate/extract, 7.5% SDS-PAGE gel, dilution 1:500.](http://images.novusbio.com/fullsize/ADAM17-Antibody-Western-Blot-NBP2-15281-img0001.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP2-15281-img0004.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TACE/ADAM17 antibody [C2C3], C-term (NBP2-15281) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15281_rabbit-polyclonal-tace-adam17-antibody-91020242014683.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Wild-type (WT) and ADAM17 knockout (KO) HeLa (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15281_rabbit-polyclonal-tace-adam17-antibody-1610202419414541.jpg)
![Western Blot: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1.](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Western-Blot-NBP2-45872-img0011.jpg "Western Blot: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1.")
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human placenta tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0010.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0001.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0002.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0003.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0004.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0005.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0006.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0007.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0008.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human testicular cancer tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0009.jpg)
![Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of extracts (35ug) from 9 different cell lines by using anti-beta Catenin monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Western-Blot-NBP2-03615-img0024.jpg "Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of extracts (35ug) from 9 different cell lines by using anti-beta Catenin monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY beta Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunocytochemistry-Immunofluorescence-NBP2-03615-img0015.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0022.jpg)
![Flow Cytometry: beta-Catenin Antibody (12H7) [NBP2-03615] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-beta Catenin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Flow-Cytometry-NBP2-03615-img0014.jpg)
![Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta Catenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-beta Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Western-Blot-NBP2-03615-img0023.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0016.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0017.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0018.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0019.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0020.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Immunohistochemistry-Paraffin-NBP2-03615-img0021.jpg)
![Flow Cytometry: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of Hela cells, using anti-beta Catenin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12H7-Flow-Cytometry-NBP2-03615-img0013.jpg)
![Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NB110-40660-img0002.jpg "Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB110-40660] - Staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Antibody at 5 ug/mL. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40660-img0001.jpg)
![Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Western-Blot-NBP3-15842-img0008.jpg "Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Western-Blot-NBP3-15842-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts from normal (control) and beta-Catenin knockout (KO) 293T cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Knockout-Validated-NBP3-15842-img0001.jpg)
![Immunoprecipitation: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug beta-Catenin antibody (NBP3-15842). Western blot was performed from the immunoprecipitate using beta-Catenin (NBP3-15842) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Immunoprecipitation-NBP3-15842-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517284937.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517184515.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517162378.jpg)
![Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from NIH/3T3 cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517223995.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from wild type(WT) and beta-Catenin knockout (KO) 293T(KO) cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517202968.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-522025171893.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517223986.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human solitary fibrous tumor tissue using [KO Validated] beta-Catenin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517172874.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human prostate cancer tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-5220251718979.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517273553.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517284993.jpg)
![Western Blot: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Breast cancer cell lysates were probed with Mouse anti-Human Beta-Catenin antibody (#NBP2-53366-20ug) at 0.5 ug/mL, followed by 1:5,000 anti-Mouse secondary antibody. Image is courtesy of customer review.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Western-Blot-NBP2-53366-img0005.jpg "Western Blot: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Breast cancer cell lysates were probed with Mouse anti-Human Beta-Catenin antibody (#NBP2-53366-20ug) at 0.5 ug/mL, followed by 1:5,000 anti-Mouse secondary antibody. Image is courtesy of customer review.")
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded Rat Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Immunohistochemistry-Paraffin-NBP2-53366-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded human Pancreas stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Immunohistochemistry-Paraffin-NBP2-53366-img0001.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Immunohistochemistry-Paraffin-NBP2-53366-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded Mouse Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Immunohistochemistry-Paraffin-NBP2-53366-img0003.jpg)
![Western Blot: JMJD6/PSR Antibody [NBP1-71693] - Analysis of JMJD6 in A431 cell lysate.](http://images.novusbio.com/fullsize/JMJD6-PSR-Antibody-Western-Blot-NBP1-71693-img0008.jpg "Western Blot: JMJD6/PSR Antibody [NBP1-71693] - Analysis of JMJD6 in A431 cell lysate.")
![Immunocytochemistry/Immunofluorescence: JMJD6/PSR Antibody [NBP1-71693] - JMJD6 antibody was tested at 10 ug/ml in A431 cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/JMJD6-PSR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71693-img0007.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO celllysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO celllysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - HeLa whole cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0003.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - Analysis of TACE in HeLa (A, D), Jurkat (B, E) and Raji (C,F) cell lysate in (A-C) the absence or (D-F) the presence of blocking peptide with TACE antibody at 0.5 ug/mL.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - IAV via Protein Expression Profile in Cells Lines. Antibodies: TACE and GAPDH. Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044] - Figure 5 Immunocytochemistry Validation of TACE/ADAM17 in HeLa CellsImmunohistochemical analysis of HeLa cells using anti-TACE/ADAM17 antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77044_rabbit-polyclonal-tace-adam17-antibody-2711202419201577.jpg)
![Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044] - Figure 4 Immunofluorescence Validation of TACE/ADAM17 in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE/ADAM17 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).](http://images.novusbio.com/fullsize/nbp1-77044_rabbit-polyclonal-tace-adam17-antibody-271120242045673.jpg)
![Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mouse mAb against A549 (1), HepG2 (2), and C6 (3) cell lysate.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Western-Blot-NBP2-52424-img0009.jpg "Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mouse mAb against A549 (1), HepG2 (2), and C6 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis of Hela cells using BMP4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Immunocytochemistry-Immunofluorescence-NBP2-52424-img0006.jpg)
![Flow Cytometry: BMP-4 Antibody (3C11C7) [NBP2-52424] - Flow cytometric analysis of Hela cells using BMP4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Flow-Cytometry-NBP2-52424-img0010.jpg)
![Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Western-Blot-NBP2-52424-img0004.jpg)
![Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against human BMP4 recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Western-Blot-NBP2-52424-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Immunofluorescence analysis of Hela cells using BMP4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-Immunocytochemistry-Immunofluorescence-NBP2-52424-img0005.jpg)
![ELISA: BMP-4 Antibody (3C11C7) [NBP2-52424] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11C7-ELISA-NBP2-52424-img0007.jpg)