| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61894-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89196-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34206-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25161SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41249-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95096-0.02ml | |||
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0007.jpg "Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of HepG2 cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0005.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0006.jpg)
![Western Blot: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis using SYN1 mouse mAb against NIH/3T3 (1), U251 (2), C6 (3), A549 (4), and MCF-7 (5) cell lysate.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Western-Blot-NBP2-61894-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of Hela cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7B1D9) [NBP2-61894] - Analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-Immunocytochemistry-Immunofluorescence-NBP2-61894-img0004.jpg)
![ELISA: Synapsin I Antibody (7B1D9) [NBP2-61894] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7B1D9-ELISA-NBP2-61894-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89100-img0007.jpg "Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human endometrium shows no positivity as expected.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0012.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human pancreas shows strong nuclear positivity in islet cells.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0005.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in granular layer](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0009.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human stomach shows strong nuclear positivity in a subset glandular cells.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0011.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T](http://images.novusbio.com/fullsize/ARC-ARG3.1-Antibody-Western-Blot-NBP1-56929-img0010.jpg "Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T")
![Immunohistochemistry-Paraffin: ARC/ARG3.1 Antibody [NBP1-56929] - Human adrenal tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/ARC-ARG3.1-Antibody-Immunohistochemistry-Paraffin-NBP1-56929-img0004.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/ARC-ARG3.1-Antibody-Western-Blot-NBP1-56929-img0005.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Hela, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/ARC-ARG3.1-Antibody-Western-Blot-NBP1-56929-img0007.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/ARC-ARG3.1-Antibody-Western-Blot-NBP1-56929-img0008.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Sample Type: 721_B Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/ARC-ARG3.1-Antibody-Western-Blot-NBP1-56929-img0009.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0002.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human testis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0001.jpg)
![Western Blot: Vanilloid R1/TRPV1 Antibody [NB100-98897] - HPV status and EphrinB1 contribute to neurite outgrowth. Western blot analysis of whole tumor lysates from mice bearing SCC1 or SCC1 EphrinB1 tumors. Western blots repeated at least n = 4 times with technical replicates. Western blots have been cropped for clarity and conciseness. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-018-06640-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Western-Blot-NB100-98897-img0006.jpg "Western Blot: Vanilloid R1/TRPV1 Antibody [NB100-98897] - HPV status and EphrinB1 contribute to neurite outgrowth. Western blot analysis of whole tumor lysates from mice bearing SCC1 or SCC1 EphrinB1 tumors. Western blots repeated at least n = 4 times with technical replicates. Western blots have been cropped for clarity and conciseness. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-018-06640-0) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98897] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to 3d cytoplasmic loop TRPV1 (VR1, capsaicin receptor, vanilloid receptor 1): whole serum (NB100-98897) at 1: 300 dilution incubated overnight at 4C.](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-Paraffin-NB100-98897-img0005.jpg)
![Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NB100-98897] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to 3d cytoplasmic loop TRPV1 (VR1, capsaicin receptor, vanilloid receptor 1): whole serum (NB100-98897) at 1: 300 dilution incubated overnight at 4C.](http://images.novusbio.com/fullsize/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-Paraffin-NB100-98897-img0003.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Analysis in human cerebral cortex and pancreas tissues . Corresponding OPA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Analysis in human cerebral cortex and pancreas tissues . Corresponding OPA1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: OPA1 Antibody [NBP2-34206] - Staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34206-img0006.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0007.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0009.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0010.jpg)
![Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Western-Blot-NBP3-16840-img0005.jpg "Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of mouse eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0004.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Rat retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0001.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Mouse retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0002.jpg)
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of rat eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of paraffin-embedded rat eye using RPE65 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16840_rabbit-rpe65-mab-5x1f6-52202511361295.jpg)
![Western Blot: S-arrestin Antibody (S128) [NBP2-25161] - Blot of bovine retinal extracts. The antibody stains a band corresponing to S-arrestin at about 48 kDa.](http://images.novusbio.com/fullsize/S-arrestin-Antibody-S128-Western-Blot-NBP2-25161-img0004.jpg "Western Blot: S-arrestin Antibody (S128) [NBP2-25161] - Blot of bovine retinal extracts. The antibody stains a band corresponing to S-arrestin at about 48 kDa.")
![Immunocytochemistry/Immunofluorescence: S-arrestin Antibody (S128) [NBP2-25161] - Confocal image of a pig retina stained with NBP2-25161 (green). S-arrestin is most abundant in the outer segments (OS) and inner surface of the outer nuclear layer (ONL), and can be used to identify components of rod photoreceptor cells. (Cone photoreceptors have a different arrestin isotype). Other retinal layers are inner segments (IS), outer plexiform layer (OPL), inner nuclear layer (INL) and inner plexiform layer (IPL). The red stain is Fox2, an RNA binding nuclear protein related to Fox3/NeuN, which stains nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA was revealed with DAPI (blue).](http://images.novusbio.com/fullsize/S-arrestin-Antibody-S128-Immunocytochemistry-Immunofluorescence-NBP2-25161-img0003.jpg)
![Western Blot: Arrestin 3 Antibody [NBP2-41249] - Analysis of ARR3 in EL4 cell lysate with ARR3 antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/Arrestin-3-Antibody-Western-Blot-NBP2-41249-img0001.jpg "Western Blot: Arrestin 3 Antibody [NBP2-41249] - Analysis of ARR3 in EL4 cell lysate with ARR3 antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Arrestin 3 Antibody [NBP2-41249] - ARR3 in mouse spleen tissue with ARR3 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/Arrestin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41249-img0004.jpg)
![Immunohistochemistry: Arrestin 3 Antibody - BSA Free [NBP2-41249] - Immunohistochemistry of Arrestin 3 in mouse spleen tissue with Arrestin 3 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41249_rabbit-polyclonal-arrestin-3-antibody-271120241955307.gif)
![Western Blot: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Lane A: Marker; Lane B HepG2 Cell Lysate. Antibody titration 1.0ug/ml. Gel concentration 12%](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Western-Blot-NBP1-69270-img0005.jpg "Western Blot: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Lane A: Marker; Lane B HepG2 Cell Lysate. Antibody titration 1.0ug/ml. Gel concentration 12%")
![Immunohistochemistry: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Chicken embryos Primary Antibody: 1 : 1000 (NBP1-69270) anti -shh Secondary Antibody: 1 : 500 goat anti-rabbit HRP conjugated.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Immunohistochemistry-NBP1-69270-img0009.jpg)
![Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Human Intestine cells with positive label: Epithelial cells of intestinal villus (indicated with arrows). Concentration 4.0-8.0 ug/ml](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Immunohistochemistry-Paraffin-NBP1-69270-img0002.jpg)
![Immunohistochemistry: Sonic Hedgehog/Shh Antibody [NBP1-69270] - Human glioma cells. Primary Antibody Dilution :1:200 Secondary Antibody :Anti-rabbit-GFP. Secondary Antibody Dilution :1:500. Color/Signal Descriptions :1. SHH: Green 2. DAPI: Blue 3. Merge. Gene Name :SHH](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-Immunohistochemistry-NBP1-69270-img0007.jpg)
![Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.](http://images.novusbio.com/fullsize/RPE65-Antibody-Western-Blot-NBP2-95096-img0003.jpg "Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.")
![Immunohistochemistry: RPE65 Antibody [NBP2-95096] - Analysis of Mouse eye cells using RPE65 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-Immunohistochemistry-NBP2-95096-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519302965.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-5220251931373.jpg)
![Western Blot: RPE65 Antibody - BSA Free [NBP2-95096] - Western blot analysis of lysates from Mouse eye, using RPE65 Rabbit pAb (A9841) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519294553.jpg)
![Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-26820241525104.jpg "Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: SOX2 Antibody (PO00-28) [NBP3-32972] - Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-28820241249344.jpg)
![Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-298202414161844.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-29202410334048.jpg)