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![Western Blot: Gamma Adaptin Antibody [NB110-7877] - Analysis shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. System was used for signal detection (Roche) using a 60-sec exposure time.](http://images.novusbio.com/fullsize/Gamma Adaptin Antibody-Western Blot-NB110-7877-img0003.jpg "Western Blot: Gamma Adaptin Antibody [NB110-7877] - Analysis shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. System was used for signal detection (Roche) using a 60-sec exposure time.")
![Western Blot: Dynamin 3 Antibody [NBP2-42836] - Analysis of A. 50 ug Rat testis lysate/extract 7.5 % SDS-PAGE Dynamin3 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/Dynamin-3-Antibody-Western-Blot-NBP2-42836-img0001.jpg "Western Blot: Dynamin 3 Antibody [NBP2-42836] - Analysis of A. 50 ug Rat testis lysate/extract 7.5 % SDS-PAGE Dynamin3 antibody dilution: 1:1000.")
![Western Blot: Dynamin 3 Antibody [NBP2-42836] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 3 antibody (NBP2-42836) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-42836_rabbit-polyclonal-dynamin-3-antibody-91020242091443.jpg)
![Immunoprecipitation:Gamma Adaptin AntibodyNBP3-30665] -Detection of human gamma Adaptin/AP1G1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-gamma Adaptin/AP1G1 antibody NBP3-30665 (lot NBP3-30665-1) used for IP at 6 µg per reaction. gamma Adaptin/AP1G1 was also immunoprecipitated by rabbit anti-gamma Adaptin/AP1G1 antibody. For blotting immunoprecipitated gamma Adaptin/AP1G1, NBP3-30665 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30665_rabbit-gamma-adaptin-pab-301220241318264.jpg "Immunoprecipitation:Gamma Adaptin AntibodyNBP3-30665] -Detection of human gamma Adaptin/AP1G1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-gamma Adaptin/AP1G1 antibody NBP3-30665 (lot NBP3-30665-1) used for IP at 6 µg per reaction. gamma Adaptin/AP1G1 was also immunoprecipitated by rabbit anti-gamma Adaptin/AP1G1 antibody. For blotting immunoprecipitated gamma Adaptin/AP1G1, NBP3-30665 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot:[NBP3-29102]-Detection of human and mouse CCT2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T , Jurkat , HeLa and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CCT2 antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. CCT2 was also immunoprecipitated by rabbit anti-CCT2 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds and 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29102_rabbit-tcp1-beta-pab-23120251252865.jpg "Western Blot:[NBP3-29102]-Detection of human and mouse CCT2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T , Jurkat , HeLa and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CCT2 antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. CCT2 was also immunoprecipitated by rabbit anti-CCT2 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds and 3 seconds.")
![Western Blot:[NBP3-30202]-Detection of human and mouse PDIA4 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDIA4 antibody used for WB at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30202_rabbit-erp72-pab-231202512101684.jpg "Western Blot:[NBP3-30202]-Detection of human and mouse PDIA4 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDIA4 antibody used for WB at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot:[NBP3-30675]-Detection of human and mouse DNAJA1 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNAJA1 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30675_rabbit-hdj2-pab-23120251252812.jpg "Western Blot:[NBP3-30675]-Detection of human and mouse DNAJA1 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNAJA1 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot:[NBP3-29192]-Detection of human and mouse CDC37 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CDC37 antibody used for WB at 0. 4 µg/ml and 1 µg/ml and used for IP at 3 µg/mg lysate. CDC37 was also immunoprecipitated by rabbit anti-CDC37 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29192_rabbit-cdc37-pab-231202512101677.jpg "Western Blot:[NBP3-29192]-Detection of human and mouse CDC37 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CDC37 antibody used for WB at 0. 4 µg/ml and 1 µg/ml and used for IP at 3 µg/mg lysate. CDC37 was also immunoprecipitated by rabbit anti-CDC37 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.")
![Western Blot:[NBP3-30233]-Detection of human and mouse PDIA4 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDIA4 antibody used for WB at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30233_rabbit-erp72-pab-23120251225657.jpg "Western Blot:[NBP3-30233]-Detection of human and mouse PDIA4 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDIA4 antibody used for WB at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:[NBP3-30732]-Detection of human CHC by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHC antibody used for IP at 6 µg per reaction. CHC was also immunoprecipitated by rabbit anti-CHC antibody. For blotting immunoprecipitated CHC, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30732_rabbit-clathrin-heavy-chain-1-chc17-pab-2312025128856.jpg "Immunoprecipitation:[NBP3-30732]-Detection of human CHC by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHC antibody used for IP at 6 µg per reaction. CHC was also immunoprecipitated by rabbit anti-CHC antibody. For blotting immunoprecipitated CHC, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot:[NBP3-30362]-Detection of human and mouse STX12 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-STX12 antibody used for WB at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30362_rabbit-syntaxin-12-pab-23120251159508.jpg "Western Blot:[NBP3-30362]-Detection of human and mouse STX12 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-STX12 antibody used for WB at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: GGA2 Antibody (1D2) [NBP2-45853] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GGA2.](http://images.novusbio.com/fullsize/GGA2-Antibody-1D2-Western-Blot-NBP2-45853-img0001.jpg "Western Blot: GGA2 Antibody (1D2) [NBP2-45853] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GGA2.")
![Western Blot: APBA1 Antibody [NBP2-41085] - Western blot analysis of UROP11 in mouse spleen tissue lysate with UROP11 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/APBA1-Antibody-Western-Blot-NBP2-41085-img0001.jpg "Western Blot: APBA1 Antibody [NBP2-41085] - Western blot analysis of UROP11 in mouse spleen tissue lysate with UROP11 antibody at (A) 1 and (B) 2 ug/mL.")