| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85302-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38622-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44496 | |||
| BSA Free | NBP2-52887-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38632-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24583SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92714SS | |||
![Western Blot: NUMB Antibody [NBP2-27181] - Analysis of mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NUMB antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution were used for this test](http://images.novusbio.com/fullsize/NUMB-Antibody-Western-Blot-NBP2-27181-img0004.jpg "Western Blot: NUMB Antibody [NBP2-27181] - Analysis of mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NUMB antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution were used for this test")
![Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human adrenal tissue stained with NUMB antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunohistochemistry-Paraffin-NBP2-27181-img0006.jpg)
![Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human spleen stained with NUMB antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunohistochemistry-Paraffin-NBP2-27181-img0005.jpg)
![Flow Cytometry: CD45 Antibody [NBP2-15811] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP2-15811-img0006.jpg "Flow Cytometry: CD45 Antibody [NBP2-15811] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50.")
![Flow Cytometry: CD45 Antibody [NBP2-15811] - Mouse splenocytes cell fixed in 4% paraformaldehyde at 4 C for 15 min. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488- Conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP2-15811-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (5C16) [NB110-93609] - Correlation of MAP17 expression with inflammation (CD45) in human samples of tumors of different originMAP17 and CD45 staining of serial sections of human samples of tumors of different origin. MAP17 staining in all samples showed high expression levels, correlated with the inflammatory marker CD45. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/21651) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-5C16-Immunohistochemistry-Paraffin-NB110-93609-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (5C16) [NB110-93609] - Correlation of MAP17 expression with inflammation (CD45) in human samples of tumors of different originMAP17 and CD45 staining of serial sections of human samples of tumors of different origin. MAP17 staining in all samples showed high expression levels, correlated with the inflammatory marker CD45. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/21651) licensed under a CC-BY license.")
![Immunohistochemistry: CD45 Antibody (5C16) [NB110-93609] - CD45 antibody (5C16) [NB110-93609] immunohistochemistry staining of paraffin sections of mouse kidney and liver tissues from LPS exposed animals.](http://images.novusbio.com/fullsize/CD45-Antibody-5C16-Immunohistochemistry-NB110-93609-img0001.jpg)
![Immunohistochemistry: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609] - Correlation of MAP17 expression with inflammation in human samples of Crohn's disease & psoriasisMAP17, CD45 & CD68 staining of histological slides of human samples of psoriasis & Crohn's disease. MAP17 staining in both inflammatory diseases showed high expression levels, correlated with the inflammatory markers CD45 & CD68. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29228712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-93609_rat-monoclonal-cd45-antibody-5c16-310202415171311.jpg)
![Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524] - Aggrecan on sheep intervertebral disc. Image from verified customer review.](http://images.novusbio.com/fullsize/Aggrecan-Antibody-6-B-4-Immunohistochemistry-Paraffin-NB110-6524-img0001.jpg "Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524] - Aggrecan on sheep intervertebral disc. Image from verified customer review.")
![Western Blot: INTS6 Antibody [NBP1-85302] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431](http://images.novusbio.com/fullsize/INTS6-Antibody-Western-Blot-NBP1-85302-img0005.jpg "Western Blot: INTS6 Antibody [NBP1-85302] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431")
![Immunocytochemistry/Immunofluorescence: INTS6 Antibody [NBP1-85302] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/INTS6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85302-img0007.jpg)
![Immunohistochemistry-Paraffin: INTS6 Antibody [NBP1-85302] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/INTS6-Antibody-Immunohistochemistry-Paraffin-NBP1-85302-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: INTS6 Antibody [NBP1-85302] - Analysis in human testis and skeletal muscle tissues. Corresponding INTS6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/INTS6-Antibody-Immunohistochemistry-Paraffin-NBP1-85302-img0008.jpg)
![Immunohistochemistry-Paraffin: INTS6 Antibody [NBP1-85302] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/INTS6-Antibody-Immunohistochemistry-Paraffin-NBP1-85302-img0009.jpg)
![Immunohistochemistry-Paraffin: INTS6 Antibody [NBP1-85302] - Staining of human prostate shows weak nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/INTS6-Antibody-Immunohistochemistry-Paraffin-NBP1-85302-img0010.jpg)
![Immunohistochemistry-Paraffin: INTS6 Antibody [NBP1-85302] - Staining of human skeletal muscle shows low positivity in myocytes as expected.](http://images.novusbio.com/fullsize/INTS6-Antibody-Immunohistochemistry-Paraffin-NBP1-85302-img0011.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP2-38622 (A) shows similar protein distribution across tissues to independent antibody NBP1-84687 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0012.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP2-38622-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0014.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0015.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human pancreas shows weak to moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0018.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RUNX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Western-Blot-NBP2-52887-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RUNX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of RH-30 cells using the Anti-RUNX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunocytochemistry-Immunofluorescence-NBP2-52887-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells outside the reaction centrum.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0004.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Western-Blot-NBP2-52887-img0005.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human rectum shows nuclear positivty in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0001.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human small intestine shows nuclear immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0002.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human testis shows absence of staining (negative control).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0003.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human endometrium, kidney, liver and placenta using Anti-SFRP4 antibody (A) shows similar protein distribution across tissues to independent antibody (B).](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0017.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human endometrium, kidney, liver and placenta using Anti-SFRP4 antibody (A) shows similar protein distribution across tissues to independent antibody (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: sFRP-4 Antibody [NBP2-38632] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Western-Blot-NBP2-38632-img0002.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human endometrium shows moderate secreted positivity in cells in endometrial stroma.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0012.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0013.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0014.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0015.jpg)
![Western Blot: CDX2 Antibody [NBP2-24583] - Total protein from human HeLa, HepG2, CaCo2 and HCT116, mouse 3T3 and Neuro2A and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CDX2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/CDX2-Antibody-Western-Blot-NBP2-24583-img0003.jpg "Western Blot: CDX2 Antibody [NBP2-24583] - Total protein from human HeLa, HepG2, CaCo2 and HCT116, mouse 3T3 and Neuro2A and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CDX2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: CDX2 Antibody [NBP2-24583] - Formalin-fixed, paraffin-embedded human spleen probed with CDX2 / CDX3 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/CDX2-Antibody-Immunohistochemistry-Paraffin-NBP2-24583-img0002.jpg)
![Western Blot: CDX2 Antibody [NBP2-24583] - Analysis of CDX3/CDX2 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/CDX2-Antibody-Western-Blot-NBP2-24583-img0001.jpg)
![Western Blot: GAP-43 Antibody [NBP1-92714] - Western blot analysis of different tissue and cell lysates using chicken pAb to GAP43, NBP1-92714, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at the 43kDa mark corresponds to GAP43. The GAP43 protein only is detected in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43.](http://images.novusbio.com/fullsize/GAP-43-Antibody-NBP1-92714-img0002.jpg "Western Blot: GAP-43 Antibody [NBP1-92714] - Western blot analysis of different tissue and cell lysates using chicken pAb to GAP43, NBP1-92714, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at the 43kDa mark corresponds to GAP43. The GAP43 protein only is detected in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP1-92714] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to GAP43, NBP1-92714, dilution 1:2,000 in green, and costained with rabbit pAb to alpha-II spectrin, dilution 1:1,000 in red. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane and synapses of neuronal cells, while the spectrin antibody stains the submembraneous cytoskeleton of axons and dendrites.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunofluorescence-NBP1-92714-img0001.jpg)
![Western Blot: SPRY2 Antibody (1e10) [H00010253-M01] - SPRY2 monoclonal antibody (M01), clone 1E10 Analysis of SPRY2 expression in C32.](http://images.novusbio.com/fullsize/SPRY2-Antibody-1e10-Western-Blot-H00010253-M01-img0018.jpg "Western Blot: SPRY2 Antibody (1e10) [H00010253-M01] - SPRY2 monoclonal antibody (M01), clone 1E10 Analysis of SPRY2 expression in C32.")
![Immunocytochemistry/Immunofluorescence: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of monoclonal antibody to SPRY2 on HeLa cell. Antibody concentration 25 ug/ml.](http://images.novusbio.com/fullsize/SPRY2-Antibody-1e10-Immunocytochemistry-Immunofluorescence-H00010253-M01-img0014.jpg)
![Immunohistochemistry-Paraffin: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SPRY2-Antibody-1e10-Immunohistochemistry-Paraffin-H00010253-M01-img0016.jpg)
![Western Blot: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody (M01), clone 1E10.Lane 1: SPRY2 transfected lysate(34.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SPRY2-Antibody-1e10-Western-Blot-H00010253-M01-img0019.jpg)
![ELISA: SPRY2 Antibody (1e10) [H00010253-M01] - Detection limit for recombinant GST tagged SPRY2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SPRY2-Antibody-1e10-ELISA-H00010253-M01-img0020.jpg)
![Western Blot: Jagged 1 Antibody (1E12.) [H00000182-M01A] - Western Blot detection against Immunogen (35.64 KDa) .](http://images.novusbio.com/fullsize/Jagged-1-Antibody-1E12-Western-Blot-H00000182-M01A-img0001.jpg "Western Blot: Jagged 1 Antibody (1E12.) [H00000182-M01A] - Western Blot detection against Immunogen (35.64 KDa) .")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged 1 regulates learning-dependent Notch induction and is enriched presynaptically. Fluorescent double-labeling shows the expression of Notch1 (red) following spatial exploration in CTLs and Jagged 1cKO. Jagged 1 labeling (green) is used to validate the absence of Jagged 1 in the Jagged 1cKO (Jag1cKOs). Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/article/10.3389/fncel.2017.00220/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-1E12-Knockout-Validated-H00000182-M01A-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Targeted loss of Jagged1 in adult mouse neurons causes a spatial memory deficit. A schematic representation of the Jagged1 floxed allele used to generate the mice with TAM-inducible loss of Jagged1 gene (A). Representative immunofluorescence images of single confocal z-plane showing a near complete loss of Jagged1 protein (green) from hippocampal pyramidal neurons labeled by NeuN (red) of Jagged1cKO mice (B). Representative images from immunoblots showing the expression of Jagged1, DNER in Jagged1cKO mice & control mice (C). GAPDH is used as a loading control. Bar graph showing the quantitation of optical densities of Jagged1 & DNER bands in CTLs & Jagged1cKO mice (D). A graphic showing the behavioral arena for Y-maze spontaneous alternation test (E). Jagged1cKO mice show a significant deficit in hippocampus dependent working memory in the Y-maze spontaneous alternation test (F). A diagram showing the experimental arena for the hidden arm version of the Y-maze (G). Jagged1cKO mice show a significant reduction in the time spent in the hidden arm, suggesting a spatial memory defect (H). A schematic showing the experimental setup for the Novel Object Displacement test (I). Jagged1cKO mice exhibit a statistical significant reduction in discrimination index, a measure of spatial memory defect (J). Scale bar in (B) is 50 μm. Graphs are represented as mean ± SEM, *p < 0.05, **p < 0.01, & ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415485115.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged1 regulates learning-dependent Notch induction & is enriched presynaptically. Fluorescent double-labeling shows the expression of Notch1 (red) following spatial exploration in CTLs & Jagged1cKO (A). Jagged1 labeling (green) is used to validate the absence of Jagged1 in the Jagged1cKO (Jag1cKOs) (A,C). Diagram summarizing the Notch1 fluorescence intensities distribution in randomly picked 69 & 71 CA1 neurons in CTLs & Jagged1cKOs, respectively (p < 0.001) (B). Double immunofluorescence of the Notch1 transcriptional target, Hes5, in CTLs & JaggedcKOs following spatial exploration (C). Diagram summarizing the Hes5 fluorescence intensity distribution in randomly picked 64 & 70 CA1 neurons in CTL & Jagged1cKO, respectively (p < 0.001) (D). Representatives gold Immuno-electron microscopy panels from three WT mice show that Jagged1 particles are localized in presynaptic terminals, bound to presynaptic vesicles (Pre) (E–E‴). Immunogold particles are also apparent at the Postsynaptic density (Post) (E,E″,E‴). Box Plot summarizing the particle counts in presynaptic & postsynaptic terminal areas drawn on Immunoelectromicroscopy micrograph for Jagged1 indicates that the presynapse is enriched with Jag1 particles as compared to the postsynaptic terminal (p < 0.001) (F). Scale bars are: 20 μm in (A), 10 μm in (C), 400 nm in (E–E‴). AU, arbitrary units; Pre, presynaptic; Post, postsynaptic; & Ax, axon. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415483644.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged1 expression in brains & CSF of AD patients. Representative double fluorescent immunolabelings for Jagged1 (green) & Notch1 (red) counterstained with Thioflavin-T or DAPI (blue) on postmortem brain sections comprising the hippocampal CA fields from healthy age-matched controls & AD patients (A–C). in healthy controls, Jagged1 is localized to somata of neurons where also Notch1 is expressed. As expected, Thioflavin-T labeling is negligible (A,A′). AD sections show fibrillary aggregates (B,B′) & (C,C′) core plaques double positive for Notch1 & Thioflavin-T. Jag1 expression is scattered in parenchyma & low in, degenerated neurons (white arrows) (B,B′). Jag1 overlays in small double positive aggregates for Notch1 & Thioflavin-T (100x magnification) in radiating plaques with a visible reduction in Jagged1 cellular expression (B–C′). Box plots summarizing the quantification of fluorescence intensities of Jagged1 immunolabeled neurons shows a significant reduction in Jagged1 expression in AD patients (p < 0.001). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415485139.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged1 expression in brains & CSF of AD patients. Representative double fluorescent immunolabelings for Jagged1 (green) & Notch1 (red) counterstained with Thioflavin-T or DAPI (blue) on postmortem brain sections comprising the hippocampal CA fields from healthy age-matched controls & AD patients (A–C). in healthy controls, Jagged1 is localized to somata of neurons where also Notch1 is expressed. As expected, Thioflavin-T labeling is negligible (A,A′). AD sections show fibrillary aggregates (B,B′) & (C,C′) core plaques double positive for Notch1 & Thioflavin-T. Jag1 expression is scattered in parenchyma & low in, degenerated neurons (white arrows) (B,B′). Jag1 overlays in small double positive aggregates for Notch1 & Thioflavin-T (100x magnification) in radiating plaques with a visible reduction in Jagged1 cellular expression (B–C′). Box plots summarizing the quantification of fluorescence intensities of Jagged1 immunolabeled neurons shows a significant reduction in Jagged1 expression in AD patients (p < 0.001). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415482294.jpg)