Growth-and-Development reagents: Novus Biologicals

NBP1-48289

Notch-1 Antibody (OTI3E12)

(2 Reviews)

(4 Publications)

0.1 ml

NBP1-48289

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded thyroid using anti-Notch1 mouse monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded breast using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of lung using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded colon using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded endometrium using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded kidney using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded pancreas using anti-Notch1 mouse monoclonal antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73042AF350
Alexa Fluor 405	NBP2-73042AF405
Alexa Fluor 488	NBP2-73042AF488
Alexa Fluor 532	NBP2-73042AF532
Alexa Fluor 594	NBP2-73042AF594
Alexa Fluor 647	NBP2-73042AF647
Alexa Fluor 700	NBP2-73042AF700
Alexa Fluor 750	NBP2-73042AF750
Alexa Fluor Plus 405	NBP2-73042AFP405
Alexa Fluor Plus 488	NBP2-73042AFP488
Alexa Fluor Plus 555	NBP2-73042AFP555
Alexa Fluor Plus 594	NBP2-73042AFP594
Alexa Fluor Plus 647	NBP2-73042AFP647
Alexa Fluor Plus 680	NBP2-73042AFP680
Allophycocyanin	NBP2-73042APC
Biotin	NBP2-73042B
CoraFluor 1	NBP2-73042CL1
DyLight 350	NBP2-73042UV
DyLight 405	NBP2-73042V
DyLight 488	NBP2-73042G
DyLight 550	NBP2-73042R
DyLight 594	NBP2-73042DL594
DyLight 650	NBP2-73042C
DyLight 680	NBP2-73042FR
DyLight 755	NBP2-73042IR
FITC	NBP2-73042F
HRP	NBP2-73042H
Janelia Fluor 525	NBP2-73042JF525
Janelia Fluor 549	NBP2-73042JF549
Janelia Fluor 646	NBP2-73042JF646
Janelia Fluor 669	NBP2-73042JF669
PE	NBP2-73042PE
PerCP	NBP2-73042PCP
mFluor Violet 450 SE	NBP2-73042MFV450
mFluor Violet 500 SE	NBP2-73042MFV500
mFluor Violet 610 SE	NBP2-73042MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73042

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NBP2-43560

Vimentin Antibody (812) - Azide and BSA Free

(5 Publications)

0.1 ml

NBP2-43560

Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Analysis of Frozen section of embryonic mouse brain (mE18.5). Red: Vimentin antibody [812] diluted at 1:250. Blue: DAPI.

Western Blot: Vimentin Antibody (812) [NBP2-43560] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract 10 % SDS-PAGE Vimentin antibody [812] dilution: 1:1000

Sandwich ELISA: Vimentin Antibody (812) [NBP2-43560] - Sandwich ELISA detection of recombinant full-length Vimentin protein using Vimentin Antibody (812) as capture antibody at concentration of 5 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.

Western Blot: Vimentin Antibody (812) [NBP2-43560] - Non-transfected (-) and transfected (+) 293T whole cell extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

ELISA: Vimentin Antibody (812) [NBP2-43560] - Sandwich ELISA detection of recombinant full-length Vimentin protein using NBP2-43560 as capture antibody at concentration of 5 ug/mL and detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.

Western Blot: Vimentin Antibody (812) [NBP2-43560] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP2-43560) diluted at 1:500000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:3000.

<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:5000.

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:75000.

Reactivity:

Hu, Mu, Rt, Fe, Bv, Ca, Ch, Pm, Gp

Species Glossary

Reactivity: Hu, Mu, Rt, Fe, Bv, Ca, Ch, Pm, Gp

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB500-178

Numb Antibody - BSA Free

(7 Publications)

0.1 ml

NB500-178

Immunocytochemistry/Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Numb Antibody (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Characterisation of LNX1 interacting proteins.(A) Schematic diagram of the domain structure of LNX1p80 and LNX2 showing the RING and four PDZ domains. N represents the NUMB-binding NPAY/NPAF motif. (B) The ability of the indicated proteins to interact with transfected GFP-tagged LNX constructs was assessed in HEK 293 cells. For each interacting protein, top panels show western blots of cell lysates (Lys), while the bottom panels show the output of a GFP “pull down

Reactivity:

Hu, Mu, Ch, Rt

Species Glossary

Reactivity: Hu, Mu, Ch, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-178AF350
Alexa Fluor 405	NB500-178AF405
Alexa Fluor 488	NB500-178AF488
Alexa Fluor 532	NB500-178AF532
Alexa Fluor 594	NB500-178AF594
Alexa Fluor 647	NB500-178AF647
Alexa Fluor 700	NB500-178AF700
Alexa Fluor 750	NB500-178AF750
Alexa Fluor Plus 405	NB500-178AFP405
Alexa Fluor Plus 488	NB500-178AFP488
Alexa Fluor Plus 555	NB500-178AFP555
Alexa Fluor Plus 594	NB500-178AFP594
Alexa Fluor Plus 647	NB500-178AFP647
Alexa Fluor Plus 680	NB500-178AFP680
Allophycocyanin	NB500-178APC
Biotin	NB500-178B
CoraFluor 1	NB500-178CL1
DyLight 350	NB500-178UV
DyLight 405	NB500-178V
DyLight 488	NB500-178G
DyLight 550	NB500-178R
DyLight 594	NB500-178DL594
DyLight 650	NB500-178C
DyLight 680	NB500-178FR
DyLight 755	NB500-178IR
FITC	NB500-178F
HRP	NB500-178H
Janelia Fluor 525	NB500-178JF525
Janelia Fluor 549	NB500-178JF549
Janelia Fluor 646	NB500-178JF646
Janelia Fluor 669	NB500-178JF669
PE	NB500-178PE
PerCP	NB500-178PCP
mFluor Violet 450 SE	NB500-178MFV450
mFluor Violet 500 SE	NB500-178MFV500
mFluor Violet 610 SE	NB500-178MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB500-178SS

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NBP1-97670

Vimentin Antibody (V9) - BSA Free

(5 Publications)

0.1 mg

NBP1-97670

Flow Cytometry: Vimentin Antibody (V9) [NBP1-97670] - An intracellular stain was performed on Jurkat cells with Vimentin antibody (V9) NBP1-97670PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of glomeruli and connetive tissue in human kidney with in a 1:200 dilution. No reactivity in epithelial cells.

Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of connective tissue cells in swine colon with in a 1:200 dilution. No reactivity in epithelial cells.

Flow Cytometry: Vimentin Antibody (V9) [NBP1-97670] - Analysis of PE conjugate of NBP1-97670. An intracellular stain was performed on THP-1 cells with Vimentin antibody (V9) NBP1-97670PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% s

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in human tonsil. NBP1-97670 was diluted to 0.5ug/mL and was left on sections for 30m at room temperature. Secondary antibody was Horse-Anti Mouse HRP Veterinary Reagent. Image from verified customer review.

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in horse intestine. NBP1-97670 was used at a concentration of 0.5ug/mL and was left on slides for 30m at room temperature. Secondary antibody was Horse Anti-Mouse HRP Veterinary Reagent. Image from verified customer review.

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in feline intestine. NBP1-97670 was used at a concentration of 0.5ug/mL. Secondary antibody was Horse Anti-Mouse HRP Veterinary Reagent. Image from verified customer review.

Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in canine intestine. NBP1-97670 was used at a concentration of 0.5ug/mL and was left on tissue sections for 30m at room temperature. Secondary was Horse Anti-Mouse Veterinary Reagent. Image from verified customer review.

Immunohistochemistry-Paraffin: Mouse Monoclonal Vimentin Antibody (V9) [NBP1-97670] - Vimentin was stained in human FFPE tonsil tissue. HIER antigen retrieval at pH 9 for 20min was performed. Concentration used: 1:100. AF750 conjugated version of the antibody was used (Catalog # NBP1-97670AF750). Image from a verified customer review.

The effect of N-BLR knockdown on invasion by specific siRNAs. a N-BLR abundance is decreased in stably silenced clones. b Invasion assays at 36 h show significant reduction of stably silenced N-BLR invading cells. c Migration assay at 24 h identified also significant reduction in migration of stably silenced N-BLR clones. d The 12 most significantly differentially expressed genes for both upregulated and downregulated genes. The data originated from 44 K Agilent microarray where HCT116 stable shRNA N-BLR clones #3-1 and #4-7 were compared with HCT116 empty vector control clone. The probes recognizing E-cadherin and vimentin are in red and blue, respectively. e Confirmation of microarray data by real time PCR shows that E-cadherin is increased and vimentin is markedly decreased in stably silenced clones (#3-1 and #4-7). f E-cadherin, vimentin, and ZEB1 were identified in vitro by immunofluorescence with specific antibodies. Immunofluorescence signal of E-cadherin (green color) was markedly increased in both clones. The ZEB1 signal was present in cells with empty vector (green color) but not in clones #3-1 and #4-7. Blue color indicate nuclei. Single green, blue, and merged channel images of ZEB1 are reported in Additional file 3: Figure S9B. g ZEB1 mRNA downregulation in HCT116 stable shRNA N-BLR clones #3-1 and #4-7 compared with control HCT116 empty vector clone. h Western blotting for E-cadherin and ZEB1 measured in the same clones; vinculin was used as loading control. (Student’s t-test; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28535802), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Rt, Bv, Ca, Ch, Eq, Fe, Sh

Species Glossary

Reactivity: Hu, Rt, Bv, Ca, Ch, Eq, Fe, Sh

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-97670AF350
Alexa Fluor 405	NBP1-97670AF405
Alexa Fluor 488	NBP1-97670AF488
Alexa Fluor 532	NBP1-97670AF532
Alexa Fluor 594	NBP1-97670AF594
Alexa Fluor 647	NBP1-97670AF647
Alexa Fluor 700	NBP1-97670AF700
Alexa Fluor 750	NBP1-97670AF750
Alexa Fluor Plus 405	NBP1-97670AFP405
Alexa Fluor Plus 488	NBP1-97670AFP488
Alexa Fluor Plus 555	NBP1-97670AFP555
Alexa Fluor Plus 594	NBP1-97670AFP594
Alexa Fluor Plus 647	NBP1-97670AFP647
Alexa Fluor Plus 680	NBP1-97670AFP680
Allophycocyanin	NBP1-97670APC
Biotin	NBP1-97670B
CoraFluor 1	NBP1-97670CL1
DyLight 350	NBP1-97670UV
DyLight 405	NBP1-97670V
DyLight 488	NBP1-97670G
DyLight 550	NBP1-97670R
DyLight 594	NBP1-97670DL594
DyLight 650	NBP1-97670C
DyLight 680	NBP1-97670FR
DyLight 755	NBP1-97670IR
FITC	NBP1-97670F
HRP	NBP1-97670H
Janelia Fluor 525	NBP1-97670JF525
Janelia Fluor 549	NBP1-97670JF549
Janelia Fluor 646	NBP1-97670JF646
Janelia Fluor 669	NBP1-97670JF669
PE	NBP1-97670PE
PerCP	NBP1-97670PCP
mFluor Violet 450 SE	NBP1-97670MFV450
mFluor Violet 500 SE	NBP1-97670MFV500
mFluor Violet 610 SE	NBP1-97670MFV610

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NBP1-97671

Vimentin Antibody (RV203) - BSA Free

(4 Publications)

0.1 mg

NBP1-97671

Flow Cytometry: Vimentin Antibody (RV203) [NBP1-97671] - An intracellular stain was performed on HeLa cells with NBP1-97671PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) [NBP1-97671] - Paraffin section of human colon

Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Figure 3. Immunohistochemistry with NBP1-97671 (RV203) on a frozen section of human kidney showing positive staining in blood vessels, glomeruli and connective tissue cells and no reactivity in epithelial cells.

Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.A: Ki67, 20X, insert- 5X digital (LCAS-R-12 weeks post-injection) B: Oct3/4, 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) C: Nestin 20x, insert- 5X digital (LCAS-R-12 weeks post-injection) D: Nestin 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) E: Sox2 40X, insert- 5X digital, (LC26-R-12 weeks post-injection) F: Sox2 40X, insert- 5X digital (LCAS-R-12 weeks post-injection) G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - Analysis of RG, TCL & the TCL derived tumors(A1, A2) LCAS-R 2nd nsphrs/LCAS-RTL(138) 2nd nsphr, (A3) Tubb3 40x LCAS-RTL(138) 2nd nsphr, (A4) GFAP 40x LCAS-RTL(138) 2nd nsphr; (B1, B2) HE 40x LCAS-R/LCAS-RTL(138) - images show undifferentiated small round blue cells with scarce cytoplasm, (B3) HE 40x (LCAS-RTL(138) SVZ 15 weeks post-injection) - extensive proliferation of undifferentiated embryonal tumor cells infiltrating the adjacent brain parenchyma, (B4) Ki67 40x (LCAS-RTL(138) SVZ 15 weeks post-injection) - high proliferative activity of tumor cells is reflected by over 75% of cells expressing Ki-67, (C1) OCT3/4 40x (LC26-RTL(170) SVZ 12 weeks post-injection) - extensive proliferation of tumor cells invading adjacent brain parenchyma show high expression of OCT3/4 (over 80% of cells), (C2) Sox2 40x (LC26-RTL(170) SVZ 12 weeks post-injection) - extensively expressed in the tumor cells, (C3) PRAME 40x (LCAS-R 12 weeks post-injection) - extensively expressed in the tumor cells, (C4) Nestin 40x (LCAS-RTL(138) 15 weeks post-injection) - extensively expressed in the tumor cells, (D1) Vimentin 40x (LCAS-RTL(138) 15 weeks post-injection) - extensively expressed in the tumor cells within the main tumor mass & in the invading areas, (D2) BLBP 40x (LC26-RTL(170) 16 weeks post-injection) - extensively expressed in the tumor cells, (D3, D4) OTX2 20x, 40x (LC26-RTL(170) 16 weeks post-injection) - extensive expression (over 80% of cells) in tumor cells. TU-tumor; P-parenchyma; PI- perivascular invasion. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.24460), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Ca, Gt, Ha

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca, Gt, Ha

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-97671AF350
Alexa Fluor 405	NBP1-97671AF405
Alexa Fluor 488	NBP1-97671AF488
Alexa Fluor 532	NBP1-97671AF532
Alexa Fluor 594	NBP1-97671AF594
Alexa Fluor 647	NBP1-97671AF647
Alexa Fluor 700	NBP1-97671AF700
Alexa Fluor 750	NBP1-97671AF750
Alexa Fluor Plus 405	NBP1-97671AFP405
Alexa Fluor Plus 488	NBP1-97671AFP488
Alexa Fluor Plus 555	NBP1-97671AFP555
Alexa Fluor Plus 594	NBP1-97671AFP594
Alexa Fluor Plus 647	NBP1-97671AFP647
Alexa Fluor Plus 680	NBP1-97671AFP680
Allophycocyanin	NBP1-97671APC
Biotin	NBP1-97671B
CoraFluor 1	NBP1-97671CL1
DyLight 350	NBP1-97671UV
DyLight 405	NBP1-97671V
DyLight 488	NBP1-97671G
DyLight 550	NBP1-97671R
DyLight 594	NBP1-97671DL594
DyLight 650	NBP1-97671C
DyLight 680	NBP1-97671FR
DyLight 755	NBP1-97671IR
FITC	NBP1-97671F
HRP	NBP1-97671H
Janelia Fluor 525	NBP1-97671JF525
Janelia Fluor 549	NBP1-97671JF549
Janelia Fluor 646	NBP1-97671JF646
Janelia Fluor 669	NBP1-97671JF669
PE	NBP1-97671PE
PerCP	NBP1-97671PCP
mFluor Violet 450 SE	NBP1-97671MFV450
mFluor Violet 500 SE	NBP1-97671MFV500
mFluor Violet 610 SE	NBP1-97671MFV610

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NLS1380

CXCR4 Antibody - BSA Free

(1 Review)

(5 Publications)

0.05 ml

NLS1380

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human colon, carcinoma.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Human Lymph Node, Non-Hodgkins Lymphoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Frozen: CXCR4 Antibody [NLS1380] - Rat brain tissue sections. Rat Nucleus Accumbens treated with MDPV. Images taken with fluorescent microscope. Primary antibody was diluted to 1:200. Secondary antibody used was donkey anti-Rabbit Alexa Fluor 594 at 1:500. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Lung, small cell carcinoma

Reactivity:

Hu, Mu, Rt, Ch, Ha, Pm, Pm, Rb, Pm, Ma-Op, Pm-Or, Tr

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Ha, Pm, Pm, Rb, Pm, Ma-Op, Pm-Or, Tr

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NB400-149

PDZK1 Antibody - BSA Free

(5 Publications)

0.1 ml

NB400-149

Immunohistochemistry: PDZK1 Antibody [NB400-149] - IHC analysis of PDZK1 in mouse kidney using DAB with hematoxylin counterstain.

Simple Western: PDZK1 Antibody [NB400-149] - Simple Western lane view shows a specific band for PDZK1 in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

Reactivity:

Hu, Mu, Bv, Rt(-)

Species Glossary

Reactivity: Hu, Mu, Bv, Rt(-)

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB400-149SS

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NBP1-77067

CXCR4 Antibody - BSA Free

(5 Publications)

0.1 mg

NBP1-77067

<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).

Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.

Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.

Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.

Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.

Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.

Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.

Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-125-1

Alt Catalog# IMG-125-1

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77067AF350
Alexa Fluor 405	NBP1-77067AF405
Alexa Fluor 488	NBP1-77067AF488
Alexa Fluor 532	NBP1-77067AF532
Alexa Fluor 594	NBP1-77067AF594
Alexa Fluor 647	NBP1-77067AF647
Alexa Fluor 700	NBP1-77067AF700
Alexa Fluor 750	NBP1-77067AF750
Alexa Fluor Plus 405	NBP1-77067AFP405
Alexa Fluor Plus 488	NBP1-77067AFP488
Alexa Fluor Plus 555	NBP1-77067AFP555
Alexa Fluor Plus 594	NBP1-77067AFP594
Alexa Fluor Plus 647	NBP1-77067AFP647
Alexa Fluor Plus 680	NBP1-77067AFP680
Allophycocyanin	NBP1-77067APC
Biotin	NBP1-77067B
CoraFluor 1	NBP1-77067CL1
DyLight 350	NBP1-77067UV
DyLight 405	NBP1-77067V
DyLight 488	NBP1-77067G
DyLight 550	NBP1-77067R
DyLight 594	NBP1-77067DL594
DyLight 650	NBP1-77067C
DyLight 680	NBP1-77067FR
DyLight 755	NBP1-77067IR
FITC	NBP1-77067F
HRP	NBP1-77067H
Janelia Fluor 525	NBP1-77067JF525
Janelia Fluor 549	NBP1-77067JF549
Janelia Fluor 646	NBP1-77067JF646
Janelia Fluor 669	NBP1-77067JF669
PE	NBP1-77067PE
PerCP	NBP1-77067PCP
mFluor Violet 450 SE	NBP1-77067MFV450
mFluor Violet 500 SE	NBP1-77067MFV500
mFluor Violet 610 SE	NBP1-77067MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77067SS

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NBP2-27202

14-3-3 Antibody - BSA Free

(1 Review)

0.1 mg

NBP2-27202

Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.

Reactivity:

Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, Ze

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6796A

Alt Catalog# IMG-6796A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-27202SS

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NB100-56168

Survivin Antibody - BSA Free

(4 Publications)

0.05 ml

NB100-56168

Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168] - Analysis of Human Prostate Cancer array at 1:2000 using Survivin Antibody [NB100-56168]. A1 is a high magnification of A. Differential expression of Survivin is seen in tissue cores from four different prostate cancer patients. Hematoxylin-eosin counterstain.

Survivin was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-Survivin Whole Serum (Catalog # NB100-56168) at a 1:500 dilution overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Fe, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Fe, Pm

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5755

Alt Catalog# IMG-5755

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56168SS

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H00000860-M01

RUNX2/CBFA1 Antibody (1D8) - Azide and BSA Free

(6 Publications)

0.1 mg

H00000860-M01

Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in SJCRH30 ( Cat # L027V1 ).

Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in K-562 ( Cat # L009V1 ).

Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - RUNX2 monoclonal antibody (M01), clone 1D8. Analysis of RUNX2 expression in Jurkat.

Sandwich ELISA: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Detection limit for recombinant GST tagged RUNX2 is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NB300-186

IGSF4A/SynCAM1/CADM1 Antibody - BSA Free

(2 Publications)

0.1 ml

NB300-186

$Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Rat brain and HEK cell lysates showing different glycosylation patterns. Brain fractions give banding patterns of a core glycosylation population around 50kDa and a slightly higher glycosylated population around 65 kDa. The heterologous HEK cell lysates give a single, fully glycosylated, band around 107kDa.$

Simple Western: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Simple Western lane view shows a specific band for SynCAM in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND-75 male (top) and female (bottom) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group (SynCAM: 1 outlier in CN-CTRL males). Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND35 male (top) and female (middle) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group. Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05, **p<0.01, ***p<0.001 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND-75 male (top) and female (bottom) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group (SynCAM: 1 outlier in CN-CTRL males). Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-186SS

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Notch-1 Antibody (OTI3E12)

Vimentin Antibody (812) - Azide and BSA Free

Numb Antibody - BSA Free

Vimentin Antibody (V9) - BSA Free

Vimentin Antibody (RV203) - BSA Free

CXCR4 Antibody - BSA Free

PDZK1 Antibody - BSA Free

CXCR4 Antibody - BSA Free

14-3-3 Antibody - BSA Free

Survivin Antibody - BSA Free

RUNX2/CBFA1 Antibody (1D8) - Azide and BSA Free

IGSF4A/SynCAM1/CADM1 Antibody - BSA Free