| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40877SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-302SS | |||
| Azide and BSA Free | NBP2-80675 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-171-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30141SS | |||
| Azide and BSA Free | NBP2-80769 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-336SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25274 | |||
| BSA Free | NBP2-27332SS | |||
| Azide and BSA Free | NBP2-25274SS | |||
| Azide and BSA Free | NBP2-80982 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56340SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-108SS | |||
| Azide and BSA Free | NBP2-80674 | |||
![Western Blot: FOXL2 Antibody [NB100-1277] - (1ug/ml) staining of Mouse Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/antibody/nb100-1277_goat-polyclonal-foxl2-antibody-imgenex-img-3228-197202382424.jpg "Western Blot: FOXL2 Antibody [NB100-1277] - (1ug/ml) staining of Mouse Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: FOXL2 Antibody [NB100-1277] - Analysis of FOXL2 in PFA-fixed, E14.5 mouse ovary section using anti-FOXL2 antibody (magenta), germ cell marker (green) and hoechst (grey). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunofluorescence-NB100-1277-img0020.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - (8ug/ml) staining of paraffin embedded Human Ovary. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/antibody/nb100-1277_goat-polyclonal-foxl2-antibody-imgenex-img-3228-immunohistochemistry-paraffin-197202382651..jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - Negative Control showing staining of paraffin embedded Human Ovary, with no primary antibody.](http://images.novusbio.com/fullsize/antibody/nb100-1277_goat-polyclonal-foxl2-antibody-imgenex-img-3228-immunohistochemistry-paraffin-19720238287..jpg)
![Western Blot: Perilipin-2/ADFP Antibody - BSA Free [NB110-40877] - Western blot images (A) & quantifications (B–I) of muscle samples from WT & UCHL1 smKO mice for lipase ATGL (B), HSL (C), & MAGL (D), the fatty acid transport protein CD36 (E), the key lipid synthesis enzyme DGAT2 (F), perilipin 3 (G), perilipin 2 (H), & UCHL1 (I). n = 4-5 per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35464088), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40877_rabbit-polyclonal-perilipin-2-adfp-antibody-310202415171328.jpg)
![Western Blot: Perilipin-2/ADFP Antibody - BSA Free [NB110-40877] - Western blot images (A) & quantifications (B–G) of cell lysates from C2C12 myotubes with control or UCHL1 siRNA knockdown for perilipin 2 (B), perilipin 5 (C), CD36 (D), HSL (E), MAGL (F), & SDHB (G). n = 4 per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35464088), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40877_rabbit-polyclonal-perilipin-2-adfp-antibody-310202415384159.jpg)
![Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody - BSA Free [NB110-40877] - Lack of MT4-MMP in patrolling monocytes leads to the accumulation of Mafb+AIM+ macrophages in incipient atherosclerotic plaques. a Representative images of transverse sections of aortic sinus from Ldlr–/– mice transplanted with MT4-MMP+/+ (MT4+/+) or MT4-MMP–/– (MT4–/–) BM cells & fed a HFD for 1 week; sections were labeled for Mac3 (green), Mafb (red), & AIM (white), & with Hoechst (blue; nuclei); scale bar, 10 µm. b Number of Mac3+ cells (left), Mac3+Mafb+ cells (middle), & Mac3+Mafb+AIM+ cells (right) in the plaques of BM-transplanted Ldlr–/– mice fed a HFD for 1 week. c Representative images of transverse sections of aortic sinus from BM-transplanted Ldlr–/– mice fed a HFD for 1 week; sections were labeled for Mac3 (green), Mafb (red), & adipophilin (white), & with Hoechst (blue; nuclei); scale bar, 10 µm. d Relative % of adipophilin-positive & adipophilin-negative cells within the Mac3+Mafb+ population of BM-transplanted Ldlr–/– mice (1 week on HFD); n = 7 mice per genotype in two independent experiments. Data were tested by two-tailed Student’s t-test in b & by Fisher’s exact test in d. Results are expressed as mean ± SEM. *p < 0.05 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03351-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40877_rabbit-polyclonal-perilipin-2-adfp-antibody-310202416235522.jpg)
![Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-Western-Blot-NB600-302-img0022.jpg "Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, clone 9E10) at 1:50 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-Immunohistochemistry-Paraffin-NB600-302-img0009.jpg)
![Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-Simple-Western-NB600-302-img0006.jpg)
![Western Blot: c-Myc Antibody (9E10) - BSA Free [NB600-302] - Western blotting analysis of unsorted (W) and sorted ROSl degrees w(L) and ROShigh(H) cells. Data shown are representative of at least two independent experiments.DyLight 650 conjugated version of this antibody used (NB600-302C). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25857523/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-BSA-Free-Western-Blot-NB600-302-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc (9E10) at 10 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-Immunocytochemistry-Immunofluorescence-NB600-302-img0011.jpg)
![Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.. Using the PE format of this antibody.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-Flow-Intracellular-NB600-302-img0024.jpg)
![Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-Western-Blot-NB600-302-img0005.jpg)
![Western Blot: c-Myc Antibody (9E10) [NB600-302] - Flow cytometric analysis of ROS and the mitochondrial status. Western blotting analysis of unsorted (W) and sorted P1 and P2 cells. Data shown are representative of at least two independent experiments. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/ncomms7750) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-Western-Blot-NB600-302-img0025.jpg)
![Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-Flow-Cytometry-NB600-302-img0014.jpg)
![Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-Flow-Cytometry-NB600-302-img0019.jpg)
![Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.. Using the FITC format of this antibody.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-Flow-Intracellular-NB600-302-img0023.jpg)
![Western Blot: c-Myc Antibody (9E10) - BSA Free [NB600-302] - NLRP2 interacts with SCMC components TLE6, OOEP, FILIA & NLRP5.(A) NLRP2 was overexpressed with myc-tagged OOEP, TLE6, NLRP5 & FILIA in HEK293T cells for 48 hours, immunoprecipitated with anti-NLRP2 or IgG as negative control & immunoblotted with anti-myc. Top panel shows specificity of anti-NLRP2 IP & bottom panel shows that NLRP2 binds to OOEP, TLE6, FILIA & NLRP5. Uncropped, full length western blots have been provided in Supplementary Figure 5. (B) Whole-mount immunofluorescence co-staining with anti-NLRP2 (green) & DAPI (blue) for nuclear staining on Nlrp2+/+ oocytes & embryos at 2-, 4-,16-cell & morula stages revealed a predominantly SCMC-like localization for NLRP2. Scale bars represent 200 μm. (C) Co-staining with anti-TLE6 (green) & DAPI (blue) of paraffin-embedded oocyte sections reveals a typical cortical stain of TLE6 in oocytes of Nlrp2+/+ dams but a more intense & diffuse stain in oocytes of Nlrp2tm1a/tm1a dams. Scale bars represent 10 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep44667), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-302_mouse-monoclonal-c-myc-antibody-9e10-310202416163779.jpg)
![Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-335-img0006.jpg "Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the Alexa Fluor 488 format of this antibody.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Immunocytochemistry-Immunofluorescence-NB600-335-img0007.jpg)
![Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.4 ug/mL (1:25,000).](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-335-img0005.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) staining for Histone H2AX [p Ser139] in skin specimens from p73+/+ and p73-/- mice 10 days after wounding. All scale bars represent 50 um. Regions of the skin are labeled as: IFE, HF, epidermal wound edge (WE), and newly-formed epidermis of the wound (W); and the dotted line indicates the border between the WE and W. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0218458), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0022.jpg "Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) staining for Histone H2AX [p Ser139] in skin specimens from p73+/+ and p73-/- mice 10 days after wounding. All scale bars represent 50 um. Regions of the skin are labeled as: IFE, HF, epidermal wound edge (WE), and newly-formed epidermis of the wound (W); and the dotted line indicates the border between the WE and W. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0218458), licensed under a CC-BY license.")
![Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-2280-img0008.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Telomere dysfunctional induced foci (TIF) in HBV-related multistep hepatocarcinogenesis and the correlations thereof with stathmin and elongation factor 1alpha (EF1alpha) expression. A. Representative features of colocalization of Histone H2AX [p Ser139] and telomeric DNA in defined lesions of human multistep hepatocarcinogenesis. TIF are indicated by colored arrowheads: blue, DAPI; green, gamma H2AX; red, telomeres; yellow, TIF. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-12-154) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0021.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of human Histone H2AX [p Ser139] antibody by immunohistochemistry. Sample: FFPE section of human seminoma. Antibodies: Affinity purified rabbit Histone H2AX [p Ser139] antibody used at a dilution of 1:500. Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-Paraffin-NB100-2280-img0023.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of Mouse Histone H2AX [p Ser139] by Immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibodies: Affinity purified rabbit Histone H2AX [p Ser139] antibody. Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0018.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody was used at 1:20 dilution on Jurkat lysate(s).](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-2280-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Treatment with anti-thyroid drug protected RPE & photoreceptors from oxidative damage induced by NaIO3.RPE & retinal oxidative damage were evaluated by immunofluorescence labeling of p-gamma H2AX & 8-OHdG on the RPE whole mounts & retinal sections at 3 days post-NaIO3 injection. a Shown are representative images of p-gamma H2AX immunofluorescence labeling on the RPE whole mounts. b Shown are representative images of p-gamma H2AX & 8-OHdG immunofluorescence labeling on the retinal sections, & corresponding quantitative analysis for p-gamma H2AX labeling. ONL, outer nuclear layer; INL, inner nuclear layer. Data represented the mean ± SEM for 5 mice per group (*p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932580), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415175243.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Treatment with anti-thyroid drug protected RPE & photoreceptors from oxidative damage induced by NaIO3.RPE & retinal oxidative damage were evaluated by immunofluorescence labeling of p-gamma H2AX & 8-OHdG on the RPE whole mounts & retinal sections at 3 days post-NaIO3 injection. a Shown are representative images of p-gamma H2AX immunofluorescence labeling on the RPE whole mounts. b Shown are representative images of p-gamma H2AX & 8-OHdG immunofluorescence labeling on the retinal sections, & corresponding quantitative analysis for p-gamma H2AX labeling. ONL, outer nuclear layer; INL, inner nuclear layer. Data represented the mean ± SEM for 5 mice per group (*p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932580), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415171325.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - RES rescued DXR-induced apoptosis through DNA-damage-P63-Caspase3 pathway in mouse oocytes. (A) Representative immunofluorescence images showing the expression of gamma -H2AX in mouse oocytes. Green, gamma -H2AX, Blue, DNA, Bar = 20 μm. (B) The relative immunofluorescence intensity of gamma -H2AX was measured in control, DXR-treated & RES-supplemented oocytes. Experiments were repeated at least 3 times with more than 30 oocytes examined for each group. Data were presented as means ± S.E.M of three independent experiments. **means P < 0.01, *** means P < 0.001. (C) Protein levels of gamma -H2AX, P63 & Active-Caspase3 were examined by Western blotting in control, DXR-treated & RES-supplemented oocytes. GAPDH was used as a loading control. The clean backgrounds for the active-Caspase-3, gamma -H2AX & GAPDH is due to the exposure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32352929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-3102024165725.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Hepatic activation of FOXO3 induces oxidative damage & Akt activation. a Expression of Bcl2l11 (Bim), Sesn2 & Sesn3 by qPCR in livers from 9-week-old control & FOXO3CAHep transgenic mice (n = 4). b Representative IHC staining for 8-OHdG (bar = 100 μm) & quantification of 8-OHdG-positive cells as a percentage of total hepatocyte cells in livers of patients in area of small cells (SC) & large cells (LC). c Representative IHC staining for phospho-gamma H2AX (bar = 100 μm) & quantification of phospho-gamma H2AX-positive cells as a percentage of total hepatocyte cells in livers of patients in area of small cells & large cells. d Expression of Msh2 by qPCR (upper panel), Western blot analysis for MSH2 (middle panel) & densitometric quantification (lower panel) in livers from 9-week-old control & FOXO3CAHep transgenic mice (n = 4). e Western blot analysis in livers from 9-week-old control & FOXO3CAHep mice for serine-473 phospho-Akt, with Akt & GAPDH as loading control (left panel), as well as densitometric quantification (right panel). f Representative IHC staining for phospho-Akt in livers from 9-week-old control & FOXO3CAHep mice (bar = 100 μm). g Western blot analysis in livers from 9-week-old control & FOXO3CAHep mice for Rictor & GAPDH as loading control (left panel), as well as densitometric quantification (right panel) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31488102), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415541940.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-2280] - RES rescued DXR-induced apoptosis through DNA-damage-P63-Caspase3 pathway in mouse oocytes. (A) Representative immunofluorescence images showing the expression of gamma -H2AX in mouse oocytes. Green, gamma -H2AX, Blue, DNA, Bar = 20 μm. (B) The relative immunofluorescence intensity of gamma -H2AX was measured in control, DXR-treated & RES-supplemented oocytes. Experiments were repeated at least 3 times with more than 30 oocytes examined for each group. Data were presented as means ± S.E.M of three independent experiments. **means P < 0.01, *** means P < 0.001. (C) Protein levels of gamma -H2AX, P63 & Active-Caspase3 were examined by Western blotting in control, DXR-treated & RES-supplemented oocytes. GAPDH was used as a loading control. The clean backgrounds for the active-Caspase-3, gamma -H2AX & GAPDH is due to the exposure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32352929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241554196.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Oocyte-specific deletion of Gsk-3 beta disrupted early folliculogenesis in mice. aGsk-3 beta efficiently & specifically deleted in the oocytes in cKO mouse ovary. Immunofluorescence staining for GSK-3 beta (green) & DDX4 (red) on 1 dpp ovary. The nucleus stained by Hoechst (blue). b Control & cKO ovaries at the indicated developmental stages. Oocytes stained w/ DDX4 (green). The nucleus stained by Hoechst (blue). c Statistical analysis showed that the total number of primordial follicle decreased significantly in 7 dpp cKO ovaries (Additional file 8: Individual data values). d Apoptotic cells increased in 1 dpp cKO ovaries compared w/ the control ovaries. TUNEL signals (green) marked apoptotic cells. The nucleus stained by Hoechst (blue). e DSBs persisted in the oocytes of 1 dpp cKO ovaries. The sections stained w/ gamma -H2AX (green) & DDX4 (red). The nucleus stained by Hoechst (blue). f Ectopic RAD51 expression in the oocytes of 1 dpp cKO ovaries. The sections stained w/ RAD51 (green) & DDX4 (red). The nucleus stained by Hoechst (blue). g, h beta -catenin accumulated in the cytoplasm & translocated into nucleus of the oocytes in cKO mice. g The sections stained w/ beta -catenin (green) & DDX4 (red). The nucleus stained by Hoechst (blue). Arrowheads indicated oocytes showing nuclear beta -catenin accumulation. h The statistic analysis demonstrated that the percentage of oocytes showing beta -catenin accumulation per section increased significantly in cKO mice (Additional file 8: Individual data values). The data are presented as mean ± s.d. The asterisk (*) denotes a statistically significant difference between the control & treatment groups. *P < 0.05, **P < 0.01, & ***P < 0.001 (t test). Scale bars, 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866939), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-3102024165746.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - IDO1 inhibitor enhances the efficacy of radiotherapy in vivo. 1 × 105 of SiHa tumorsphere cells were subcutaneously injected to nude mice for tumor growth. After the tumor volume reached 50 mm3, the mice were divided into four groups of non-treated (Ctrl), INCB-024360 treated (INCB), radiotherapy (Rx), or INCB-024360 plus radiotherapy (INCB + Rx). For the INCB or INCB + Rx group, mice were injected once with 50 mg/kg INCB-024360 intraperitoneally before radiotherapy. For the Rx or INCB + Rx group, mice received 2 Gy radiation per day for total 10 Gy. Mice were sacrificed at day 30 after the last radiation treatment & the xenografted tumors were taken out for picturing (A) & weighting (B). The expression of Ki-67 or p-gamma H2AXser139 was determined by paraffin section followed by immunohistochemical staining (C). The inserted bars indicated 50 μM. The quantification results were performed by TissueFAX software (D). * p < 0.05; ** p < 0.01. The experiments were repeated two times & data from one experiment were presented. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32545442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241612641.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Premature upregulation of TAp63 in GSK-3 beta -inhibited ovary. a Expression pattern of gamma -H2AX in fetal & neonatal mouse ovary in vivo. Mouse ovaries from 13.5 dpc, 15.5 dpc, 17.5 dpc, & 1 dpp immunostained for gamma -H2AX (green) & DDX4 (red). Nucleus stained by Hoechst (blue). gamma -H2AX displayed intensive expression in germ cell nucleus from 15.5 to 17.5 dpc. b Expression pattern of TAp63 in fetal & neonatal mouse ovary in vivo. Mouse ovaries from 13.5 dpc, 15.5 dpc, 17.5 dpc, 18.5 dpc, & 1 dpp immunostained for TAp63 (green) & DDX4 (red). Nucleus stained by Hoechst (blue). TAp63 protein located w/in somatic cells in fetal ovary & began to express in germ cell nucleus from 18.5 dpc afterward. c–e TAp63 expression upregulated in fetal ovary & displayed premature localization w/in oocyte nucleus following GSK-3 beta inhibition. Before examination, ovaries of 14.5 dpc cultured in vitro w/ DMSO or BIO for 3 days. c qRT-PCR analysis of TAp63 mRNA level following GSK-3 beta inhibition (normalized to beta -actin) (Additional file 8: Individual data values). d WB analysis of TAp63 protein level following GSK-3 beta inhibition. GAPDH used as internal control. e Immunofluorescence staining for TAp63 (green) & DDX4 (red). The nucleus stained by Hoechst (blue). TAp63 showed premature expression w/in oocyte nucleus following GSK-3 beta inhibition (arrowhead). f qRT-PCR results showed that relative mRNA expression level of p21, Bad, & Noxa increased significantly in GSK-3 beta -inhibiting ovaries (Additional file 8: Individual data values). data are presented as mean ± s.d. The asterisk (*) denotes a statistically significant difference between the control & treatment groups. *P < 0.05, **P < 0.01, & ***P < 0.001 (t test). Scale bars, 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866939), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241612651.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0013.jpg "Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 Antibody [NB500-171] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NB500-171 and 25 uL of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody (No Ab) was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive, and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Chromatin-Immunoprecipitation-NB500-171-img0016.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse testis.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0017.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - MDA-MB-231 cells were treated with vehicle (V) or paclitaxel (P). Cytosolic and nuclear lysates were prepared, and immunoblot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0020.jpg)
![Immunohistochemistry: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-NB500-171-img0012.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, and in vivo from aged donors. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a and b) and adult donors. Anti- used at 1:20,000 and Anti-Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] (NBP2-16848) used at 1:10,000. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1038/s41598-020-59163-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-171 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-171 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - Immunofluorescence staining to detect histone H3 in human blood. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0023.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 antibody on Mouse pancreas tissue. Histone H3 antibody dilution 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0025.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - (1) HeLa, (2) S. cerevisiae whole cell lysates, (3) Histones purified from HeLa cells. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0014.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Total histone H3 levels in MCF-7 and HCT-116 cells. Observed molecular weight is ~17 kDa. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0019.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat pancreas.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0009.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat colon.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse brain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0007.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - p53 but not ATM is required for chronic gamma -radiation-induced histone reductions. (a) Western blots of histone proteins in wild-type, TP53−/− & ATM−/− RPE-1 cells following exposures to 20 mGy/h chronic radiation for 7 days. (b) ATM activation, as shown by auto-phosphorylation on S1981, in response to chronic radiation in primary fibroblasts. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-31020241539715.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma -radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-31020241534368.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, & in vivo from aged donors. (a) Phase-contrast images of primary fibroblasts induced into senescence by chronic gamma -radiation, oncogene over-expression or exhaustive replication (replicative senescence), & DNA damage from a single acute 4 Gy X-ray dose at an early time-point (1 hour) as a control for DNA damage without senescence. Scale bars 200 µm. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a & b) & adult donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-310202415291940.jpg)
![Western Blot: Histone H3 Antibody - BSA Free [NB500-171] - Chronic gamma -radiation reduces histone levels. (a) Western blot analyses of histone H2AX & gamma H2AX in primary fibroblasts exposed to various dose-rates of chronic gamma -radiation for 7 days. Cells irradiated with a single acute dose of 4 Gy X-ray were included as control. (b) Effect of chronic gamma -irradiation on H2AX levels in three different isogenic primary cell types from a different donor to that used in (a) at the same dose rates as in (a). (c) Immunoblots of other histones in chronically irradiated primary fibroblasts. (d) All significant histone level changes detected by SILAC LC-MS/MS protein analyses of samples from primary fibroblasts exposed or mock-exposed to chronic gamma -radiation for 7 days. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32042076), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-171_rabbit-polyclonal-histone-h3-antibody-310202415392548.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0012.jpg "Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.")
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunohistochemistry-Paraffin-NBP1-30141-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0011.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 (NBP1-30141) using HeLa nuclear lysate [NB800-PC9]. Observed molecular weight ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of A549 cells (fixed with methanol) using Histone H3 [Trimethyl Lys9] antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0014.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Used to perform ChIP on HeLa cells.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Chromatin-Immunoprecipitation-NBP1-30141-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - H3K9Me3 analysis of MCF7 and HCT116. Theorectical molecular weight is ~15 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0008.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0013.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0004.jpg)
![Histone H3 [Trimethyl Lys9] (6F12-H4) was detected in immersion fixed MCF7 human breast cancer cell line using Mouse anti- Histone H3 [Trimethyl Lys9] (6F12-H4) Protein G Purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-30141R) (red) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/antibody/nbp1-30141_mouse-monoclonal-histone-h3-trimethyl-lys9-antibody-6f12-h4-immunocytochemistry-immunofluorescence-2382024134224..jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-336] - Staining of K-562, U266, Daudi and normal T cells with c-Myc antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Immunocytochemistry-Immunofluorescence-NB600-336-img0010.jpg "Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-336] - Staining of K-562, U266, Daudi and normal T cells with c-Myc antibody. ICC/IF image submitted by a verified customer review.")
![Western Blot: c-Myc Antibody [NB600-336] - Detection of c-Myc in HtrA2-Myc Transfectant lysates .](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0012.jpg)
![Western Blot: c-Myc Antibody [NB600-336] - Analysis of c-Myc on Jurkat whole cell extract using NB600-336.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0004.jpg)
![Western Blot: c-Myc Antibody [NB600-336] - Lane 1: control HEK293 cell lysate. Lane 2: CMYC over-expression in HEK293 cell lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0014.jpg)
![Immunohistochemistry: c-Myc Antibody [NB600-336] - Analysis of c-Myc in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Immunohistochemistry-NB600-336-img0002.jpg)
![Western Blot: c-Myc Antibody [NB600-336] - c-Myc in MOLT-4 cells. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0006.jpg)
![Western Blot: c-Myc Antibody [NB600-336] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-cMyc antibody (NB600-336) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of c-Myc antibody bound to the blotted protein. This c-Myc antibody detected the c-Myc specific band at its expected position (48-50kDa). The signal below 10 kDa in this blot is potentially the degraded protein and we have not characterized this band.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0013.jpg)
![Western Blot: c-Myc Antibody [NB600-336] - Analysis using the HRP conjugate of NB600-336. Detection of c-Myc in NIH/3T3 cell lysates (30ug and 45ug per lane) using anti-c-Myc antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-336] - Detection of c-myc Tagged Plakoglobin by Immunofluorescence. Samples: Human microvascular endothelial cells expressing c-myc tagged plakoglobin following transient transfection.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Immunocytochemistry-Immunofluorescence-NB600-336-img0005.jpg)
![Simple Western: c-Myc Antibody [NB600-336] - Lane view shows a specific band for c-Myc in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Simple-Western-NB600-336-img0007.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Simple-Western-NB100-74435-img0012.jpg "Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining of Phospho-H2AX in A549 cells. Cells were treated with 50 uM EPE for 1 hour (left panel) or with vehicle (right control: 0.1% DMSO in media).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining using NB100-74435, treatment with paraquat and iron induces MnSOD and Phosphorylation of H2AX in RAW 264.7 macrophages. Cells were treated for 20 hours with paraquat (500 uM) and iron (200 ug/ml) and stained with anti-Phospho-H2AX antibody.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Western blot analysis of Jurkat cells (Lane 1) and Jurkat cells treated with staurosporine (Lane 2) blotted with Histone H2AX [p Ser139] antibody (3F2). Bands indicate an observed molecular weight of ~16 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Western-Blot-NB100-74435-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - STED image of MDA-MB-231 cells after 2 Gy irradiation. Blue channel: DAPI, window level [0 255]. Green channel: secondary antibody Alexa Fluor 488, window level [140 255]. Scale bar: 20 um. Image from verified customer review.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Immunofluorescence analysis using Histone H2AX [p Ser139] antibody (3F2).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - IF image of phosphorylated H2AX in gamma irradiated Hela cells.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0015.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody (3F2) was used at 10 ug/ml dilution on Jurkat lysate(s).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Simple-Western-NB100-74435-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Evidence of DNA damage & oxidative stress in islets from HFD treated mice. (a) Representative immunofluorescent staining of gamma H2AX, insulin, & DAPI for islets from each treatment group. (b) Representative immunofluorescent staining of 4-hydroxynonenal (4-HNE), insulin, & DAPI for islets from each treatment group. (c) Representative immunofluorescent staining of nitrotyrosine, insulin, & DAPI for islets from each treatment group. (d–g) qRT-PCR analysis of total islet RNA for antioxidant genes, including Gpx1, SOD1, Nrf2, & Ppargc1 alpha . (n = 3) *p < 0.05, **p < 0.01. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-03869-5), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74435_mouse-monoclonal-histone-h2ax-p-ser139-antibody-3f2-31020241534396.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - THP-1 cells were stained with TLR7 (4G) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0008.jpg "Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - THP-1 cells were stained with TLR7 (4G) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.")
![Flow (Intracellular): TLR7 Antibody (4G6) [NBP2-27332] - Analysis using the PE conjugate of NBP2-27332. Staining of TLR7 in 10^6 human BDCM cells (red) and 0.5 ug of mouse IgG1 isotype control (green). this antibody was used for this test.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Intracellular-NBP2-27332-img0009.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis using Azide Free version of NBP2-27332. THP-1 cells were stained with TLR7 (4G) NBP2-25274 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0012.jpg)
![Western Blot: TLR7 Antibody (4G6) - BSA Free [NBP2-27332] - The protein of TLR7 were pull down by miR-138-biotin / miR-mut-138-biotin with Streptavidin agarose beads in BV2 cells. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC7710131/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-BSA-Free-Western-Blot-NBP2-27332-img0017.jpg)
![Immunohistochemistry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of TLR7 [4G6] antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Immunohistochemistry-NBP2-27332-img0016.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Ramos cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0015.jpg)
![Western Blot: TLR7 Antibody (4G6) [NBP2-27332] - TLR7 antibodies tested at 2 ug/ml on recombinant partial hTLR7 protein (amino acids 562-839). NB100-56682 and NBP2-24767 immunogen sequences are not present in recombinant protein; *NB100-56588 is not recommended for use in western blot.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Western-Blot-NBP2-27332-img0005.jpg)
![Western Blot: TLR7 Antibody (4G6) [NBP2-27332] - Analysis using Azide Free version of NBP2-27332. Human TLR7 antibody in Ramos and transfected 293T lysate using TLR7 monoclonal antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Western-Blot-NBP2-27332-img0011.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis of TLR7 in human monocytes using 2 ug of TLR7 monoclonal antibody (red) and 2 ug of mouse IgG1 isotype control antibody (green). TLR intracellular flow kit was used for this test, and an anti-mouse IgG PE conjugated secondary antibody .](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0002.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Daudi cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0013.jpg)
![Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Raji cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR7-Antibody-4G6-Flow-Cytometry-NBP2-27332-img0014.jpg)
![Western Blot: TLR7 Antibody (4G6) - BSA Free [NBP2-27332] - ADEV miR‐138 interacts with murine TLR7 in the endosomes. (A) Representative fluorescence images of mouse primary microglial cells incubated with Exo‐Fect+ADEVs+Cy5‐miR138 or Exo‐Fect+Cy5‐miR138 (without ADEVs) or Exo‐Fect+ADEVs+miR138 (unstained) for 30 min followed by immunostaining of (A) an early endosome marker (EEA1, Green) & (B) TLR7 (Green). Exo‐fect+ADEVs+Cy5‐miR138, Cy5‐miR138 were loaded into ADEVs using by Exo‐Fect transfection kit; Exo‐fect+Cy5‐miR138, Cy5‐miR138 were loaded using by Exo‐Fect transfection kit (without ADEVs); Exo‐fect+ADEVs+miR138, unstained miR138 were loaded into ADEVs using Exo‐Fect transfection kit; Bars, 50 μm (n = 3). (C) TLR7 was immunoprecipitated from BV2 cells by IgG / TLR7 / LAMP1 antibody, followed by assessment of miR138 / U6 expression by real‐time PCR. One‐way ANOVA followed by Bonferroni's post hoc test was used to determine the statistical significance among multiple groups (n = 3). (D) TLR7 was immunoprecipitated from HEK‐Null / HEK‐TLR7 cells by IgG/TLR7 antibody, followed by assessment of miR138 expression by real‐time PCR. One‐way ANOVA followed by Bonferroni's post hoc test was used to determine the statistical significance among multiple groups (n = 3). (E) The protein of TLR7 were pull down by miR‐138‐biotin / miR‐mut‐138‐biotin with Streptavidin agarose beads in BV2 cells. All data are presented as mean ± SD or SEM of three individual experiments. *,P < 0.05; **,P < 0.01; ***,P < 0.001 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33304479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27332_mouse-monoclonal-tlr7-antibody-4g6-310202416114724.jpg)
![Western Blot: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Knockout-Validated-NB100-56340-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NB100-56340-img0002.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NB100-56340-img0005.jpg)
![Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Simple-Western-NB100-56340-img0003.jpg)
![Western Blot: c-Myc Antibody (9E11) [NB200-108] - Expression of cancer proliferation markers and in high SVCT-2 expression cell lines. Western blot analysis of c-Myc and cyclin D1 in Sw620 and Sw480 cell lines after co-treatment with L-ascorbic acid and phloretin. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-29386-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Western-Blot-NB200-108-img0011.jpg "Western Blot: c-Myc Antibody (9E11) [NB200-108] - Expression of cancer proliferation markers and in high SVCT-2 expression cell lines. Western blot analysis of c-Myc and cyclin D1 in Sw620 and Sw480 cell lines after co-treatment with L-ascorbic acid and phloretin. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-29386-7), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: c-Myc Antibody (9E11) [NB200-108] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB200-108, clone 9E11) at 1:600 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Immunohistochemistry-Paraffin-NB200-108-img0007.jpg)
![Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U937 cells with c-Myc [9E11] Antibody NB200-108AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Flow-Cytometry-NB200-108-img0014.jpg)
![Western Blot: c-Myc Antibody (9E11) [NB200-108] - Whole cell protein from PC3 (lane 1), U-2 OS (lane 2) and mouse testis (lane 3) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-c-Myc in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Western-Blot-NB200-108-img0006.jpg)
![Western Blot: c-Myc Antibody (9E11) [NB200-108] - Expression of cancer proliferation markers in low SVCT-2 expressing cell lines. Expression of c-Myc and cyclin D1 was analyzed by western blotting in HCT15 and DLD-1 cell lines after L-ascorbic acid treatment. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-29386-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Western-Blot-NB200-108-img0012.jpg)
![Western Blot: c-Myc Antibody (9E11) [NB200-108] - Expression of cancer proliferation markers and in high SVCT-2 expression cell lines. Western blot analysis of c-Myc and cyclin D1 in Sw620 and Sw480 cell lines after co-treatment with L-ascorbic acid and phloretin. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-29386-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Western-Blot-NB200-108-img0013.jpg)
![Flow (Intracellular): c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E11) NB200-108AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Flow-Intracellular-NB200-108-img0008.jpg)
![Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E11) NB200-108PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Flow-Cytometry-NB200-108-img0009.jpg)
![Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U-937 cells with c-Myc Antibody [9E11] NB200-108 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Flow-Cytometry-NB200-108-img0010.jpg)