| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-209SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56126SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76801SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56114SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56564SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27328SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33245 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90125-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-206SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56529SS | |||
| Azide and BSA Free | NBP2-80615 | |||
![Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - Western blot analysis of Jurkat (A) and HeLa (B) cell lysate using Caspase 9 (NB500-209) antibody at 1:500.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-LAP6-96-2-22-Western-Blot-NB500-209-img0006.jpg "Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - Western blot analysis of Jurkat (A) and HeLa (B) cell lysate using Caspase 9 (NB500-209) antibody at 1:500.")
![Immunocytochemistry/Immunofluorescence: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - The Caspase 9 antibody was tested in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/Caspase-9-Antibody-LAP6-96-2-22-Immunocytochemistry-Immunofluorescence-NB500-209-img0003.jpg)
![Immunohistochemistry-Paraffin: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - IHC analysis of paraffin-embedded human breast carcinomausing Caspase 9 antibody at 1:25. Image from verified customer review.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-LAP6-96-2-22-Immunohistochemistry-Paraffin-NB500-209-img0008.jpg)
![Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-LAP6-96-2-22-Western-Blot-NB500-209-img0002.jpg)
![Simple Western: Caspase-9 Antibody (LAP6 96-2-22) [NB500-209] - Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Staurosporine (STS) for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for Caspase 9 at approximately 53 kDa (as indicated) using 20 ug/mL of Mouse Anti-Caspase 9 Monoclonal Antibody (Catalog # NB500-209). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-LAP6-96-2-22-Simple-Western-NB500-209-img0010.jpg)
![Western Blot: Caspase-14 Antibody [NB100-56126] - Analysis of Caspase-14. Tissue lysates (50 ug/lane) and recombinant human Caspase-14 were (Hu C14, 15 ng) were western blotted with Caspase-14 antibody at 1:2000. The antibody detected both the proform of caspase-14, and the large and small subunits of active/cleaved caspase-14.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-Western-Blot-NB100-56126-img0006.jpg "Western Blot: Caspase-14 Antibody [NB100-56126] - Analysis of Caspase-14. Tissue lysates (50 ug/lane) and recombinant human Caspase-14 were (Hu C14, 15 ng) were western blotted with Caspase-14 antibody at 1:2000. The antibody detected both the proform of caspase-14, and the large and small subunits of active/cleaved caspase-14.")
![Immunohistochemistry: Caspase-14 Antibody [NB100-56126] - Tissue sections of mouse skin at E17 stained using this antibody at 1:500.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-Immunohistochemistry-NB100-56126-img0007.jpg)
![Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126] - Human ovarian cancer tissue microarray stained for Caspase-14 expression using this antibody at 1:2000. Low (A) and high (B) stage ovarian tumor tissue cores. High magnification from areas of the tissue cores (A1 and B1). Decreased Caspase-14 expression was seen in the high grade, compared to the low grade tumor. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-Immunohistochemistry-Paraffin-NB100-56126-img0004.jpg)
![Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126] - Tissue sections of human cervix stained using this antibody at 1:2000. A. Normal cervix (squamous epithelium). B. CIN1 (low-grade squamous intraepithelial lesion, mild dysplasia). C. CIN2 (high-grade squamous intraepithelial lesion, moderate dysplasia. D. CIN3 (high-grade squamous intraepithelial lesion; severe dysplasia-carcinoma in situ. In normal cervi, caspase-14 staining was found most in the midzone layer, but was absent from the basal/parabasal cell layer where mitotically active cells are known to reside. This suggests induction of caspase-14 expression with differentiation. Caspase-14 expression declined progressively during malignant transformation as the histologic severity of the cervical atypia advanced from CIN1 to CIN3. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-Immunohistochemistry-Paraffin-NB100-56126-img0005.jpg)
![Western Blot: Caspase-12 Antibody [NBP1-76801] - Western blot analysis of Caspase-12 in (A) human spleen and (B) mouse spleen tissue lysate with Caspase-12 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/Caspase-12-Antibody-Western-Blot-NBP1-76801-img0005.jpg "Western Blot: Caspase-12 Antibody [NBP1-76801] - Western blot analysis of Caspase-12 in (A) human spleen and (B) mouse spleen tissue lysate with Caspase-12 antibody at 1 ug/mL.")
![Immunohistochemistry: Caspase-12 Antibody - BSA Free [NBP1-76801] - Immunohistochemical staining of mouse liver tissue using caspase-12 antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-76801_rabbit-polyclonal-caspase-12-antibody-2711202419183820.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-12 Antibody - BSA Free [NBP1-76801] - Immunofluorescence of Caspase-12 in Mouse Liver cells with Caspase-12 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76801_rabbit-polyclonal-caspase-12-antibody-2711202419432274.jpg)
![Western Blot: Caspase-6 Antibody [NB100-56114] - Analysis of Caspase-6 using this antibody. Recombinant catalytically active Caspase-6 was western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected both the large and small subunits of Caspase-6.](http://images.novusbio.com/fullsize/Caspase-6-Antibody-Western-Blot-NB100-56114-img0006.jpg "Western Blot: Caspase-6 Antibody [NB100-56114] - Analysis of Caspase-6 using this antibody. Recombinant catalytically active Caspase-6 was western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected both the large and small subunits of Caspase-6.")
![Immunohistochemistry-Paraffin: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue sections of human gliomas stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1:2000. Gemistocytoma (low grade tumor) at A) low and B) high magnification. Two examples of anaplastic, high grade (III) infiltrating gliomas (C and D). Increased active/cleaved Caspase-6 may be seen in high grade, compared to low grade tumors. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Caspase-6-Antibody-Immunohistochemistry-Paraffin-NB100-56114-img0008.jpg)
![Immunohistochemistry: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Caspase-6 expression at 1:2000. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Caspase-6-Antibody-Immunohistochemistry-NB100-56114-img0007.jpg)
![Western Blot: Caspase-1 Antibody [NB100-56564] - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # NB100-56564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Knockout-Validated-NB100-56564-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Caspase-1 Antibody [NB100-56564] - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # NB100-56564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Caspase-1 Antibody [NB100-56564] - Analysis of Caspase-1 using a polyclonal antibody. Human HeLa lysate probed with Caspase-1 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Western-Blot-NB100-56564-img0005.jpg)
![Immunohistochemistry-Paraffin: Caspase-1 Antibody [NB100-56564] - Human reactive lymph node stained for Caspase-1 expression (NB100-56564) using a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.](http://images.novusbio.com/fullsize/Caspase-1-Antibody-Immunohistochemistry-Paraffin-NB100-56564-img0004.jpg)
![Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Western-Blot-NBP2-67362-img0008.jpg "Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - ICC staining of Caspase-9 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primar](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0007.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of paraffin-embedded mouse spleen tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunohistochemistry-Paraffin-NBP2-67362-img0005.jpg)
![Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of Caspase-9 on different lysates using anti-Caspase-9 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: C2C12](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Western-Blot-NBP2-67362-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0003.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of paraffin-embedded human tonsil tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunohistochemistry-Paraffin-NBP2-67362-img0004.jpg)
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP2-15722-img0008.jpg "Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP2-15722-img0012.jpg)
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase-8 antibody (NBP2-15722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242015729.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242001016.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: Caspase-8 protein stained by Caspase-8 antibody (NBP2-15722) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242063388.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:100.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242001090.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242021975.jpg)
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase-8 antibody (NBP2-15722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-910202420122084.jpg)
![Western Blot: NOD2 Antibody [NBP2-27328] - Analysis of NOD2 using this antibody at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.](http://images.novusbio.com/fullsize/NOD2-Antibody-Western-Blot-NBP2-27328-img0001.jpg "Western Blot: NOD2 Antibody [NBP2-27328] - Analysis of NOD2 using this antibody at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.")
![Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-27328] - Formalin-fixed, paraffin-embedded normal human colon stained for NOD2 expression using this antibody at 1:2000. A, tissue core from a colon tissue microarray. A1, high magnification of A. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/NOD2-Antibody-Immunohistochemistry-Paraffin-NBP2-27328-img0002.jpg)
![Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis of Caspase 14 in mouse spleen lysate using Caspase 14 antibody at 1:1000. Novus's goat anti-mouse Ig HRP secondary antibody (Novus, 20101) and PicoTect ECL substrate solution (Novus, 10087K) were used for this test.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-70A1426-Western-Blot-NB100-56718-img0009.jpg "Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis of Caspase 14 in mouse spleen lysate using Caspase 14 antibody at 1:1000. Novus's goat anti-mouse Ig HRP secondary antibody (Novus, 20101) and PicoTect ECL substrate solution (Novus, 10087K) were used for this test.")
![Flow Cytometry: Caspase-14 Antibody (70A1426) [NB100-56718] - Intracellular analysis of Caspase-14 in 10^6 Jurkat cells probed with 1:500 dilution of Caspase-14 antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; red represents Caspase-14 antibody. This antibody was used for this test, and an anti-mouse IgG FITC conjugated secondary antibody.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-70A1426-Flow-Cytometry-NB100-56718-img0007.jpg)
![Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis of human breast cancer tissue using Caspase 14 antibody at a concentration of 0.1 ug/ml.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-70A1426-Western-Blot-NB100-56718-img0006.jpg)
![Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis for Caspase-14 using NB100-56718 at 2 ug/ml dilution against 10 ug of mouse spleen cell lysate.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-70A1426-Western-Blot-NB100-56718-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody [NBP1-90125] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90125-img0015.jpg "Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody [NBP1-90125] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.")
![Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human Caspase-7 Monoclonal Antibody (Catalog # NB500-106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-7 at approximately 40 kDa (as indicated) in the parental A549 cell line, but is not detectable in the knockout A549 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Knockdown-Validated-NB500-206-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human Caspase-7 Monoclonal Antibody (Catalog # NB500-106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-7 at approximately 40 kDa (as indicated) in the parental A549 cell line, but is not detectable in the knockout A549 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Western-Blot-NB500-206-img0002.jpg)
![Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Simple Western lane view shows a specific band for Caspase 7 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Simple-Western-NB500-206-img0003.jpg)
![Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-25B881-1-Western-Blot-NB100-56529-img0008.jpg "Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.")
![<b>Biological Strategies Validation. </b>Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-25B881-1-Western-Blot-NB100-56529-img0009.jpg)
![Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) [NB100-56529] - Human tonsil stained with Caspase-7 antibody at 5 ug/ml. Novus' human tonsil tissue slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-25B881-1-Immunohistochemistry-Paraffin-NB100-56529-img0007.jpg)