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![Western Blot: MCTS1 Antibody [NBP2-17242] - Non-transfected (-) and transfected (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MCTS1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MCTS1-Antibody-Western-Blot-NBP2-17242-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MCTS1 Antibody [NBP2-17242] - Non-transfected (-) and transfected (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MCTS1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: MCTS1 Antibody [NBP2-17242] - Rat spleen. MCTS1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MCTS1-Antibody-Immunohistochemistry-Paraffin-NBP2-17242-img0005.jpg)
![Western Blot: MCT1 Antibody [NBP2-17242] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/MCT1-Antibody-Western-Blot-NBP2-17242-img0001.jpg)
![Immunohistochemistry-Paraffin: MCTS1 Antibody [NBP2-17242] - Mouse lung. MCTS1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MCTS1-Antibody-Immunohistochemistry-Paraffin-NBP2-17242-img0004.jpg)
![SCAP antibody [HL2796] detects SCAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.SCAP stained by SCAP antibody [HL2796] (NBP3-48733) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48733_rabbit-scap-mab-hl2796-immunohistochemistry-paraffin-141120251843339.jpg "SCAP antibody [HL2796] detects SCAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.SCAP stained by SCAP antibody [HL2796] (NBP3-48733) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SCAP antibody [HL2796] (NBP3-48733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48733_rabbit-scap-mab-hl2796-western-blot-141120251837381.jpg)
![SCAP antibody [HL2796] detects SCAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.SCAP stained by SCAP antibody [HL2796] (NBP3-48733) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48733_rabbit-scap-mab-hl2796-immunohistochemistry-paraffin-141120251834590.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SCAP antibody [HL2796] (NBP3-48733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48733_rabbit-scap-mab-hl2796-western-blot-1411202518415218.jpg)
![Western Blot: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [HL2458] (NBP3-25500) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25500_rabbit-hemoglobin-epsilon-mab-hl2458-azide-and-bsa-free-2120249422614.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [HL2458] (NBP3-25500) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [HL2458] (NBP3-25500) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25500_rabbit-hemoglobin-epsilon-mab-hl2458-azide-and-bsa-free-2120249432877.jpg)
![Immunohistochemistry-Paraffin: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] - Hemoglobin epsilon antibody [HL2458] detects secreted Hemoglobin epsilon protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2458] (NBP3-25500) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25500_rabbit-hemoglobin-epsilon-mab-hl2458-azide-and-bsa-free-2120249374987.jpg)
![Immunohistochemistry-Paraffin: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] - Hemoglobin epsilon antibody [HL2458] detects secreted Hemoglobin epsilon protein by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2458] (NBP3-25500) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25500_rabbit-hemoglobin-epsilon-mab-hl2458-azide-and-bsa-free-212024941463.jpg)
![Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP3-12940] - Rat duodenum. DHCR7 antibody (NBP3-12940) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DHCR7-Antibody-Immunohistochemistry-Paraffin-NBP3-12940-img0003.jpg "Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP3-12940] - Rat duodenum. DHCR7 antibody (NBP3-12940) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP3-12940] - Mouse liver. DHCR7 antibody (NBP3-12940) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DHCR7-Antibody-Immunohistochemistry-Paraffin-NBP3-12940-img0002.jpg)
![Western Blot: DHCR7 Antibody [NBP3-12940] -MCF-7 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp3-12940_rabbit-dhcr7-pab-western-blot-7112024105637..png)
![Western Blot: SERCA2 ATPase Antibody [NBP1-59203] - (G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca2+ channels using rabbit anti-SERCA2 (Novus Biologicals; NBP1-59203) and rabbit anti-IP3R1 (Novus Biologicals; NB120-5908). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-Western-Blot-NBP1-59203-img0006.jpg "Western Blot: SERCA2 ATPase Antibody [NBP1-59203] - (G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca2+ channels using rabbit anti-SERCA2 (Novus Biologicals; NBP1-59203) and rabbit anti-IP3R1 (Novus Biologicals; NB120-5908). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.")
![Immunohistochemistry: SERCA2 ATPase Antibody [NBP1-59203] - Rhesus macaque spinal cord Primary Antibody Dilution: 1 : 300 Secondary Antibody: Donkey anti Rabbit 488 Secondary Antibody Dilution: 1 : 500Color/Signal Descriptions: Green: ATP2A2 Gene name: APLP2 Submitted by: Timur Mavlyutov, Ph. D. , Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-Immunohistochemistry-NBP1-59203-img0005.jpg)
![Western Blot: SERCA2 ATPase Antibody [NBP1-59203] - Human MCF-7. Titration: 0.2-1 ug/ml, Positive Control: MCF7 cell lysate.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-Western-Blot-NBP1-59203-img0004.jpg)
![Western Blot: NAB1 Antibody [NBP1-71838] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NAB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NAB1 was also immunoprecipitated by rabbit anti-NAB1 antibody NBP1-71837, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/NAB1-Antibody-Western-Blot-NBP1-71838-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NAB1 Antibody [NBP1-71838] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NAB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NAB1 was also immunoprecipitated by rabbit anti-NAB1 antibody NBP1-71837, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Simple Western: NAB1 Antibody [NBP1-71838] - Simple Western lane view shows a specific band for NAB1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NAB1-Antibody-Simple-Western-NBP1-71838-img0002.jpg)
![Western Blot: Periostin/OSF-2 Antibody (1A1) [NBP1-47922] - Periostin antibody ( 47 ) at 1:500 dilution + lysate from HeLa cells transfected with human Periostin gene expression vector.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-1A1-Western-Blot-NBP1-47922-img0004.jpg "Western Blot: Periostin/OSF-2 Antibody (1A1) [NBP1-47922] - Periostin antibody ( 47 ) at 1:500 dilution + lysate from HeLa cells transfected with human Periostin gene expression vector.")
![Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody (1A1) [NBP1-47922] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY POSTN.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-1A1-Immunocytochemistry-Immunofluorescence-NBP1-47922-img0003.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0004.jpg "Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0001.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0002.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0003.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0005.jpg)
![Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/APOJ-Clusterin-Antibody-CLI-9-Western-Blot-NBP1-97521-img0002.jpg "Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.")
![Western Blot: NPRB/NPR2 Antibody [NBP3-12239] - Western Blot of NBP3-12239. 1:500 antibody dilution in DiluObuffer. Apparent MW is 120-122 kDa.](http://images.novusbio.com/fullsize/NPRB-NPR2-Antibody-Western-Blot-NBP3-12239-img0002.jpg "Western Blot: NPRB/NPR2 Antibody [NBP3-12239] - Western Blot of NBP3-12239. 1:500 antibody dilution in DiluObuffer. Apparent MW is 120-122 kDa.")
![Immunohistochemistry-Paraffin: NPRB/NPR2 Antibody [NBP3-12239] - IF of NBP3-12239 with thin sections of mouse nose tissue. Green: Grueneberg ganglion neurons. Red: Antibody immunoreactivity. Blue: Nuclei. Straining is found on blood vessel walls.](http://images.novusbio.com/fullsize/NPRB-NPR2-Antibody-Immunohistochemistry-Paraffin-NBP3-12239-img0001.jpg)
![Immunohistochemistry-Paraffin: Gastrin Antibody [NBP3-12223] - Rat stomach-gastrin-1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 10X and 20X magnification on Leica DM4000.](http://images.novusbio.com/fullsize/Gastrin-Antibody-Immunohistochemistry-Paraffin-NBP3-12223-img0001.jpg "Immunohistochemistry-Paraffin: Gastrin Antibody [NBP3-12223] - Rat stomach-gastrin-1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 10X and 20X magnification on Leica DM4000.")
![Gastrin Antibody [NBP3-12223] - Rat stomach-gastrin-1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 10X and 20X magnification on Leica DM4000.](http://images.novusbio.com/fullsize/Gastrin-Antibody-NBP3-12223-img0002.jpg)
![ELISA: VEGF ELISA Kit [Biotin] [NBP1-92679] - Standard curve](http://images.novusbio.com/fullsize/VEGF-ELISA-Kit-[Biotin]-ELISA-NBP1-92679-img0001.jpg "ELISA: VEGF ELISA Kit [Biotin] [NBP1-92679] - Standard curve")